2015-05-11

Background The overall prognosis of colorectal cancer (CRC) patients is unsatisfactory due to cancer metastasis after operation. This study aims to investigate the clinical significance of plasma osteopontin (OPN) levels as minimally invasive, predictive, and surrogate biomarkers for prognosis of CRC patients. Methods This randomized study design consists of pre-operative and post-operative plasma samples from a total of 79 patients. We determined plasma levels of OPN by ELISA and examined their correlation with the clinicopathological parameters of CRC patients. The effects of endogenous and exogenous OPN on CRC metastasis were investigated by examination of the effect on regulators of epithelial to messenchymal transition and migration assay. Results Our findings demonstrated for the first time the clinical correlation of plasma OPN with metastasis of CRC patients. High post-operative plasma OPN level (>153.02 ng/ml) associated with development of metastasis after curative resection (p<0.001). Moreover, post-operative plasma OPN level correlated with disease-free survival of CRC patients (p=0.009) and was an independent factor for predicting development of metastasis in CRC patients after curative resection (p=0.036). Our in vitro model showed that OPN ectopic expression induced DLD1 cell migration through Snail and Twist1 overexpression and E-cadherin repression, and secretory OPN level enhanced cell migration. Conclusions The results of the current study suggest that post-operative plasma OPN correlated with post-operative metastasis, suggesting that it is a potential non-invasive biomarker for the development of future metastasis in CRC patients. In addition, OPN was shown to be involved in the metastatic process and thus inhibition of OPN is a potential therapeutic approach to treat CRC patients.

Ng, L, Wan, TMH, Lam, CSC, Chow, AKM, Wong, SKM, Man, JHW, Li, HS, Cheng, NSM, Pak, RCH, Cheung, AHK, Yau, TCC, Lo, OSH, Foo, DCC, Poon, JTC, Poon, RTP, Pang, RWC, Law, WL. (2015). Data from: Post-Operative Plasma Osteopontin Predicts Distant Metastasis in Human Colorectal Cancer. [Data File]. All relevant data are within the paper and its Supporting Information files. Click on “Linked Publications” to access the publication and access supporting information on figshare at https://figshare.com/articles/_Post_Operative_Plasma_Osteopontin_Predicts_Distant_Metastasis_in_Human_Colorectal_Cancer_/1410072

migration assay.ResultsOur findings

plasma OPN level

Human Colorectal Cancer BackgroundThe

plasma OPN

CRC patients

CRC patients.MethodsThis randomized study design

OPN ectopic expression

secretory OPN level

DLD 1 cell migration

elisa

Twist 1 overexpression

metastasi

cell migration.ConclusionsThe results