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- Publisher Website: 10.1016/j.jsb.2010.12.007
- Scopus: eid_2-s2.0-79952450056
- PMID: 21195767
- WOS: WOS:000288640100019
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Article: Structural characterization and anti-HIV-1 activities of arginine/glutamate-rich polypeptide Luffin P1 from the seeds of sponge gourd (Luffa cylindrica)
| Title | Structural characterization and anti-HIV-1 activities of arginine/glutamate-rich polypeptide Luffin P1 from the seeds of sponge gourd (Luffa cylindrica) | ||||||||||||||
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| Authors | |||||||||||||||
| Keywords | Anti-HIV-1 Luffin P1 Nuclear magnetic resonance Ribosome-inactivating peptide | ||||||||||||||
| Issue Date | 2011 | ||||||||||||||
| Publisher | Academic Press. The Journal's web site is located at http://www.elsevier.com/locate/yjsbi | ||||||||||||||
| Citation | Journal Of Structural Biology, 2011, v. 174 n. 1, p. 164-172 How to Cite? | ||||||||||||||
| Abstract | Luffin P1, the smallest ribosome-inactivating peptide from the seeds of Luffa cylindrica was found to have anti-HIV-1 activity in HIV-1 infected C8166 T-cell lines and be able to bind with HIV Rev Response Element. Nuclear magnetic resonance spectroscopy revealed that the Luffin P1 comprises a helix-loop-helix motif, with the two alpha helices tightly associated by two disulfide bonds. Based on our findings, we conclude that unlike the well-studied ribosome-inactivating proteins, which exert their action through N-glycosidase activities, Luffin P1 demonstrates a novel inactivation mechanism probably through the charge complementation with viral or cellular proteins. Our work also provides a new scaffold for the design of novel inhibitors from a simple helical motif. © 2010 Elsevier Inc. | ||||||||||||||
| Persistent Identifier | http://hdl.handle.net/10722/133607 | ||||||||||||||
| ISSN | 2021 Impact Factor: 3.234 2020 SCImago Journal Rankings: 2.564 | ||||||||||||||
| ISI Accession Number ID |
Funding Information: The work in Hong Kong was supported by grants from the Research Grants Council General Research Grant (HKU 7533/06M and 7755/08M for KH Sze and CUHK 4606/06M for PC Shaw). Yinghua Yang was supported in part by a postdoctoral fellowship from the Chinese University of Hong Kong. The purchase of the 700 MHz nuclear magnetic spectrometer was by a One-Off Special Equipment Grant (SEG CUHKO8 and CUHKO9) of the University Grants Council of Hong Kong SAR. The work in China was supported in part by grants from the National Basic Research Program of China (2009CB5223006), Eleventh Five-Year Key Scientific and Technological Program of China (2009ZX09501-029; 2008ZX10001-002, 2008ZX10001-015), Chinese Academy of Sciences (KSCX2-YW-R-185). | ||||||||||||||
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| DC Field | Value | Language |
|---|---|---|
| dc.contributor.author | Ng, YM | en_HK |
| dc.contributor.author | Yang, Y | en_HK |
| dc.contributor.author | Sze, KH | en_HK |
| dc.contributor.author | Zhang, X | en_HK |
| dc.contributor.author | Zheng, YT | en_HK |
| dc.contributor.author | Shaw, PC | en_HK |
| dc.date.accessioned | 2011-05-24T02:11:41Z | - |
| dc.date.available | 2011-05-24T02:11:41Z | - |
| dc.date.issued | 2011 | en_HK |
| dc.identifier.citation | Journal Of Structural Biology, 2011, v. 174 n. 1, p. 164-172 | en_HK |
| dc.identifier.issn | 1047-8477 | en_HK |
| dc.identifier.uri | http://hdl.handle.net/10722/133607 | - |
| dc.description.abstract | Luffin P1, the smallest ribosome-inactivating peptide from the seeds of Luffa cylindrica was found to have anti-HIV-1 activity in HIV-1 infected C8166 T-cell lines and be able to bind with HIV Rev Response Element. Nuclear magnetic resonance spectroscopy revealed that the Luffin P1 comprises a helix-loop-helix motif, with the two alpha helices tightly associated by two disulfide bonds. Based on our findings, we conclude that unlike the well-studied ribosome-inactivating proteins, which exert their action through N-glycosidase activities, Luffin P1 demonstrates a novel inactivation mechanism probably through the charge complementation with viral or cellular proteins. Our work also provides a new scaffold for the design of novel inhibitors from a simple helical motif. © 2010 Elsevier Inc. | en_HK |
| dc.language | eng | en_US |
| dc.publisher | Academic Press. The Journal's web site is located at http://www.elsevier.com/locate/yjsbi | en_HK |
| dc.relation.ispartof | Journal of Structural Biology | en_HK |
| dc.rights | Appropriate Bibliographic Citation:Authors posting Accepted Author Manuscript online should later add a citation for the Published Journal Article indicating that the Article was subsequently published, and may mention the journal title provided that they add the following text at the beginning of the document: “NOTICE: this is the author’s version of a work that was accepted for publication in <Journal title>. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in PUBLICATION, [VOL#, ISSUE#, (DATE)] DOI#” | - |
| dc.subject | Anti-HIV-1 | en_HK |
| dc.subject | Luffin P1 | en_HK |
| dc.subject | Nuclear magnetic resonance | en_HK |
| dc.subject | Ribosome-inactivating peptide | en_HK |
| dc.subject.mesh | Anti-HIV Agents - chemistry - pharmacology | - |
| dc.subject.mesh | Circular Dichroism | - |
| dc.subject.mesh | Luffa - chemistry | - |
| dc.subject.mesh | Peptides - chemistry - pharmacology | - |
| dc.subject.mesh | Seeds - chemistry | - |
| dc.title | Structural characterization and anti-HIV-1 activities of arginine/glutamate-rich polypeptide Luffin P1 from the seeds of sponge gourd (Luffa cylindrica) | en_HK |
| dc.type | Article | en_HK |
| dc.identifier.openurl | http://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1047-8477&volume=174&issue=1&spage=164&epage=172&date=2011&atitle=Structural+characterization+and+anti-HIV-1+activities+of+arginine/glutamate-rich+polypeptide+Luffin+P1+from+the+seeds+of+sponge+gourd+(Luffa+cylindrica) | - |
| dc.identifier.email | Sze, KH:khsze@hku.hk | en_HK |
| dc.identifier.authority | Sze, KH=rp00785 | en_HK |
| dc.description.nature | link_to_subscribed_fulltext | - |
| dc.identifier.doi | 10.1016/j.jsb.2010.12.007 | en_HK |
| dc.identifier.pmid | 21195767 | - |
| dc.identifier.scopus | eid_2-s2.0-79952450056 | en_HK |
| dc.identifier.hkuros | 185371 | en_US |
| dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-79952450056&selection=ref&src=s&origin=recordpage | en_HK |
| dc.identifier.volume | 174 | en_HK |
| dc.identifier.issue | 1 | en_HK |
| dc.identifier.spage | 164 | en_HK |
| dc.identifier.epage | 172 | en_HK |
| dc.identifier.isi | WOS:000288640100019 | - |
| dc.publisher.place | United States | en_HK |
| dc.relation.project | Structural and functional studies of the SWIRM domain in Lysine-specific Demethylase 1 (LSD1) | - |
| dc.identifier.scopusauthorid | Ng, YM=42462197300 | en_HK |
| dc.identifier.scopusauthorid | Yang, Y=35750932800 | en_HK |
| dc.identifier.scopusauthorid | Sze, KH=7006735061 | en_HK |
| dc.identifier.scopusauthorid | Zhang, X=36062136800 | en_HK |
| dc.identifier.scopusauthorid | Zheng, YT=35228297300 | en_HK |
| dc.identifier.scopusauthorid | Shaw, PC=35599523600 | en_HK |
| dc.identifier.citeulike | 8625089 | - |
| dc.identifier.issnl | 1047-8477 | - |