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- Publisher Website: 10.1128/MCB.00234-09
- Scopus: eid_2-s2.0-68849111429
- PMID: 19564417
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Article: PU.1 can recruit BCL6 to DNA to repress gene expression in germinal center B cells
Title | PU.1 can recruit BCL6 to DNA to repress gene expression in germinal center B cells | ||||||
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Authors | |||||||
Issue Date | 2009 | ||||||
Citation | Molecular And Cellular Biology, 2009, v. 29 n. 17, p. 4612-4622 How to Cite? | ||||||
Abstract | BCL6 is a transcriptional repressor crucial for germinal center formation. BCL6 represses transcription by a variety of mechanisms by binding to specific DNA sequences or by recruitment to DNA by protein interactions. We found that BCL6 can inhibit activities of the immunoglobulin kappa (Igκ) intron and 3′ enhancers. At the Igκ 3′ enhancer, BCL6 repressed enhancer activity through the PU.1 binding site. We found that BCL6 physically interacted with PU.1 in vivo and in vitro, and the results of sequential chromatin immunoprecipitation assays and transient-expression assays suggested that BCL6 recruitment to the Igκ and Igλ 3′ enhancers occurred via PU.1 interaction. By computational studies, we identified genes that are repressed in germinal center cells and whose promoters contain conserved PU.1 binding sites in mouse and human. We found that many of these promoters bound to both PU.1 and BCL6 in vivo. In addition, BCL6 knockdown resulted in increased expression of a subset of these genes, demonstrating that BCL6 is involved in their repression. The recruitment of BCL6 to promoter regions by PU.1 represents a new regulatory mechanism that expands the number of genes regulated by this important transcriptional repressor. Copyright © 2009, American Society for Microbiology. All Rights Reserved. | ||||||
Persistent Identifier | http://hdl.handle.net/10722/147607 | ||||||
ISSN | 2021 Impact Factor: 5.069 2020 SCImago Journal Rankings: 2.140 | ||||||
PubMed Central ID | |||||||
ISI Accession Number ID |
Funding Information: This work was supported by NIH grant GM071830 (M.L.A.) and by grant A.E./M-04/04 from the University Grants Committee of Hong Kong (J.W.). | ||||||
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Wei, F | en_US |
dc.contributor.author | Zaprazna, K | en_US |
dc.contributor.author | Wang, J | en_US |
dc.contributor.author | Atchison, ML | en_US |
dc.date.accessioned | 2012-05-29T06:04:56Z | - |
dc.date.available | 2012-05-29T06:04:56Z | - |
dc.date.issued | 2009 | en_US |
dc.identifier.citation | Molecular And Cellular Biology, 2009, v. 29 n. 17, p. 4612-4622 | en_US |
dc.identifier.issn | 0270-7306 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/147607 | - |
dc.description.abstract | BCL6 is a transcriptional repressor crucial for germinal center formation. BCL6 represses transcription by a variety of mechanisms by binding to specific DNA sequences or by recruitment to DNA by protein interactions. We found that BCL6 can inhibit activities of the immunoglobulin kappa (Igκ) intron and 3′ enhancers. At the Igκ 3′ enhancer, BCL6 repressed enhancer activity through the PU.1 binding site. We found that BCL6 physically interacted with PU.1 in vivo and in vitro, and the results of sequential chromatin immunoprecipitation assays and transient-expression assays suggested that BCL6 recruitment to the Igκ and Igλ 3′ enhancers occurred via PU.1 interaction. By computational studies, we identified genes that are repressed in germinal center cells and whose promoters contain conserved PU.1 binding sites in mouse and human. We found that many of these promoters bound to both PU.1 and BCL6 in vivo. In addition, BCL6 knockdown resulted in increased expression of a subset of these genes, demonstrating that BCL6 is involved in their repression. The recruitment of BCL6 to promoter regions by PU.1 represents a new regulatory mechanism that expands the number of genes regulated by this important transcriptional repressor. Copyright © 2009, American Society for Microbiology. All Rights Reserved. | en_US |
dc.language | eng | en_US |
dc.relation.ispartof | Molecular and Cellular Biology | en_US |
dc.subject.mesh | Animals | en_US |
dc.subject.mesh | B-Lymphocytes - Cytology - Physiology | en_US |
dc.subject.mesh | Dna - Genetics - Metabolism | en_US |
dc.subject.mesh | Enhancer Elements, Genetic | en_US |
dc.subject.mesh | Gene Expression Regulation | en_US |
dc.subject.mesh | Germinal Center - Cytology | en_US |
dc.subject.mesh | Humans | en_US |
dc.subject.mesh | Immunoglobulin Lambda-Chains - Genetics | en_US |
dc.subject.mesh | Immunoglobulins - Genetics | en_US |
dc.subject.mesh | Introns | en_US |
dc.subject.mesh | Mice | en_US |
dc.subject.mesh | Promoter Regions, Genetic | en_US |
dc.subject.mesh | Proto-Oncogene Proteins - Genetics - Metabolism | en_US |
dc.subject.mesh | Proto-Oncogene Proteins C-Bcl-6 - Genetics - Metabolism | en_US |
dc.subject.mesh | Trans-Activators - Genetics - Metabolism | en_US |
dc.title | PU.1 can recruit BCL6 to DNA to repress gene expression in germinal center B cells | en_US |
dc.type | Article | en_US |
dc.identifier.email | Wang, J:junwen@hkucc.hku.hk | en_US |
dc.identifier.authority | Wang, J=rp00280 | en_US |
dc.description.nature | link_to_OA_fulltext | en_US |
dc.identifier.doi | 10.1128/MCB.00234-09 | en_US |
dc.identifier.pmid | 19564417 | - |
dc.identifier.pmcid | PMC2725722 | - |
dc.identifier.scopus | eid_2-s2.0-68849111429 | en_US |
dc.identifier.hkuros | 158846 | - |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-68849111429&selection=ref&src=s&origin=recordpage | en_US |
dc.identifier.volume | 29 | en_US |
dc.identifier.issue | 17 | en_US |
dc.identifier.spage | 4612 | en_US |
dc.identifier.epage | 4622 | en_US |
dc.identifier.isi | WOS:000268813100003 | - |
dc.publisher.place | United States | en_US |
dc.identifier.scopusauthorid | Wei, F=55186434600 | en_US |
dc.identifier.scopusauthorid | Zaprazna, K=32868288900 | en_US |
dc.identifier.scopusauthorid | Wang, J=8950599500 | en_US |
dc.identifier.scopusauthorid | Atchison, ML=7006495633 | en_US |
dc.identifier.issnl | 0270-7306 | - |