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- Scopus: eid_2-s2.0-0020696101
- PMID: 6825676
- WOS: WOS:A1983PY02200017
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Article: Purification of 5-aminolaevulinate synthase from liver mitochondria of chick embryo.
Title | Purification of 5-aminolaevulinate synthase from liver mitochondria of chick embryo. |
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Authors | |
Issue Date | 1983 |
Publisher | Blackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journals/EJB |
Citation | European Journal Of Biochemistry, 1983, v. 129 n. 3, p. 615-620 How to Cite? |
Abstract | 5-Aminolaevulinate synthase from chick-embryo liver mitochondria has, for the first time, been purified to homogeneity in its native non-degraded form by molecular sieve chromatography, chromatofocusing and affinity chromatography. The enzyme has a minimum molecular weight of 68000 as determined by sodium dodecylsulphate/polyacrylamide gel electrophoresis and a specific activity of 35000 units/mg of protein. This result conflicts with the previous report of Whiting, M.J. and Granick, G. [(1976) J. Biol. Chem. 251, 1340-1346] that the chick embryo enzyme has a molecular weight of 49000. We show here that the purified form can be degraded proteolytically to a smaller form of molecular weight around 50000 while retaining full enzymatic activity. It seem evident, therefore, that the enzyme isolated by Whiting & Granick (1976) was degraded. We have further established by pulse-labelling studies and immunoprecipitation that the enzyme isolated by our new and rapid procedure has the same minimum molecular weight as that which exists in vivo. |
Persistent Identifier | http://hdl.handle.net/10722/147734 |
ISSN | |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Borthwick, IA | en_US |
dc.contributor.author | Srivastava, G | en_US |
dc.contributor.author | Brooker, JD | en_US |
dc.contributor.author | May, BK | en_US |
dc.contributor.author | Elliott, WH | en_US |
dc.date.accessioned | 2012-05-29T06:08:58Z | - |
dc.date.available | 2012-05-29T06:08:58Z | - |
dc.date.issued | 1983 | en_US |
dc.identifier.citation | European Journal Of Biochemistry, 1983, v. 129 n. 3, p. 615-620 | en_US |
dc.identifier.issn | 0014-2956 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/147734 | - |
dc.description.abstract | 5-Aminolaevulinate synthase from chick-embryo liver mitochondria has, for the first time, been purified to homogeneity in its native non-degraded form by molecular sieve chromatography, chromatofocusing and affinity chromatography. The enzyme has a minimum molecular weight of 68000 as determined by sodium dodecylsulphate/polyacrylamide gel electrophoresis and a specific activity of 35000 units/mg of protein. This result conflicts with the previous report of Whiting, M.J. and Granick, G. [(1976) J. Biol. Chem. 251, 1340-1346] that the chick embryo enzyme has a molecular weight of 49000. We show here that the purified form can be degraded proteolytically to a smaller form of molecular weight around 50000 while retaining full enzymatic activity. It seem evident, therefore, that the enzyme isolated by Whiting & Granick (1976) was degraded. We have further established by pulse-labelling studies and immunoprecipitation that the enzyme isolated by our new and rapid procedure has the same minimum molecular weight as that which exists in vivo. | en_US |
dc.language | eng | en_US |
dc.publisher | Blackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journals/EJB | en_US |
dc.relation.ispartof | European Journal of Biochemistry | en_US |
dc.subject.mesh | 5-Aminolevulinate Synthetase - Isolation & Purification | en_US |
dc.subject.mesh | Animals | en_US |
dc.subject.mesh | Chick Embryo | en_US |
dc.subject.mesh | Chromatography - Methods | en_US |
dc.subject.mesh | Electrophoresis, Polyacrylamide Gel | en_US |
dc.subject.mesh | Mitochondria, Liver - Enzymology | en_US |
dc.subject.mesh | Molecular Weight | en_US |
dc.title | Purification of 5-aminolaevulinate synthase from liver mitochondria of chick embryo. | en_US |
dc.type | Article | en_US |
dc.identifier.email | Srivastava, G:gopesh@pathology.hku.hk | en_US |
dc.identifier.authority | Srivastava, G=rp00365 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.pmid | 6825676 | en_US |
dc.identifier.scopus | eid_2-s2.0-0020696101 | en_US |
dc.identifier.volume | 129 | en_US |
dc.identifier.issue | 3 | en_US |
dc.identifier.spage | 615 | en_US |
dc.identifier.epage | 620 | en_US |
dc.identifier.isi | WOS:A1983PY02200017 | - |
dc.publisher.place | United Kingdom | en_US |
dc.identifier.issnl | 0014-2956 | - |