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Article: Human metapneumovirus detection in patients with severe acute respiratory syndrome

TitleHuman metapneumovirus detection in patients with severe acute respiratory syndrome
Authors
Chan, PKSTam, JSLam, CWChan, EWu, ALi, CKBuckley, TANg, KCJoynt, GMCheng, FWTTo, KFLee, NHui, DSCCheung, JLKChu, ILiu, EChung, SSCSung, JJY
Issue Date2003
PublisherUS Department of Health and Human Services, Centers for Disease Control and Prevention. The Journal's web site is located at http://www.cdc.gov/ncidod/EID/index.htm
Citation
Emerging Infectious Diseases, 2003, v. 9 n. 9, p. 1058-1063 How to Cite?
AbstractWe used a combination approach of conventional virus isolation and molecular techniques to detect human metapneumovirus (HMPV) in patients with severe acute respiratory syndrome (SARS). Of the 48 study patients, 25 (52.1%) were infected with HMPV; 6 of these 25 patients were also infected with coronavirus, and another 5 patients (10.4%) were infected with coronavirus alone. Using this combination approach, we found that human laryngeal carcinoma (HEp-2) cells were superior to rhesus monkey kidney (LLC-MK2) cells commonly used in previous studies for isolation of HMPV. These widely available HEp-2 cells should be included in conjunction with a molecular method for cell culture followup to detect HMPV, particularly in patients with SARS.
Persistent Identifierhttp://hdl.handle.net/10722/148352
ISSN
1080-6040
2021 Impact Factor: 16.126
2020 SCImago Journal Rankings: 2.540
ISI Accession Number ID
WOS:000185100900005
References
References in Scopus

 

DC FieldValueLanguage
dc.contributor.authorChan, PKSen_US
dc.contributor.authorTam, JSen_US
dc.contributor.authorLam, CWen_US
dc.contributor.authorChan, Een_US
dc.contributor.authorWu, Aen_US
dc.contributor.authorLi, CKen_US
dc.contributor.authorBuckley, TAen_US
dc.contributor.authorNg, KCen_US
dc.contributor.authorJoynt, GMen_US
dc.contributor.authorCheng, FWTen_US
dc.contributor.authorTo, KFen_US
dc.contributor.authorLee, Nen_US
dc.contributor.authorHui, DSCen_US
dc.contributor.authorCheung, JLKen_US
dc.contributor.authorChu, Ien_US
dc.contributor.authorLiu, Een_US
dc.contributor.authorChung, SSCen_US
dc.contributor.authorSung, JJYen_US
dc.date.accessioned2012-05-29T06:12:25Z-
dc.date.available2012-05-29T06:12:25Z-
dc.date.issued2003en_US
dc.identifier.citationEmerging Infectious Diseases, 2003, v. 9 n. 9, p. 1058-1063en_US
dc.identifier.issn1080-6040en_US
dc.identifier.urihttp://hdl.handle.net/10722/148352-
dc.description.abstractWe used a combination approach of conventional virus isolation and molecular techniques to detect human metapneumovirus (HMPV) in patients with severe acute respiratory syndrome (SARS). Of the 48 study patients, 25 (52.1%) were infected with HMPV; 6 of these 25 patients were also infected with coronavirus, and another 5 patients (10.4%) were infected with coronavirus alone. Using this combination approach, we found that human laryngeal carcinoma (HEp-2) cells were superior to rhesus monkey kidney (LLC-MK2) cells commonly used in previous studies for isolation of HMPV. These widely available HEp-2 cells should be included in conjunction with a molecular method for cell culture followup to detect HMPV, particularly in patients with SARS.en_US
dc.languageengen_US
dc.publisherUS Department of Health and Human Services, Centers for Disease Control and Prevention. The Journal's web site is located at http://www.cdc.gov/ncidod/EID/index.htmen_US
dc.relation.ispartofEmerging Infectious Diseasesen_US
dc.subject.meshAdulten_US
dc.subject.meshAgeden_US
dc.subject.meshAnimalsen_US
dc.subject.meshAntibodies, Viral - Isolation & Purificationen_US
dc.subject.meshCells, Cultureden_US
dc.subject.meshCommunicable Diseases, Emerging - Epidemiology - Physiopathology - Virologyen_US
dc.subject.meshCoronavirus - Isolation & Purificationen_US
dc.subject.meshDisease Outbreaksen_US
dc.subject.meshFemaleen_US
dc.subject.meshHong Kong - Epidemiologyen_US
dc.subject.meshHumansen_US
dc.subject.meshMacaca Mulattaen_US
dc.subject.meshMaleen_US
dc.subject.meshMetapneumovirus - Immunology - Isolation & Purificationen_US
dc.subject.meshMiddle Ageden_US
dc.subject.meshReverse Transcriptase Polymerase Chain Reactionen_US
dc.subject.meshSevere Acute Respiratory Syndrome - Epidemiology - Physiopathology - Virologyen_US
dc.titleHuman metapneumovirus detection in patients with severe acute respiratory syndromeen_US
dc.typeArticleen_US
dc.identifier.emailLam, CW:ching-wanlam@pathology.hku.hken_US
dc.identifier.authorityLam, CW=rp00260en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid14519240-
dc.identifier.scopuseid_2-s2.0-0042818007en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0042818007&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume9en_US
dc.identifier.issue9en_US
dc.identifier.spage1058en_US
dc.identifier.epage1063en_US
dc.identifier.isiWOS:000185100900005-
dc.publisher.placeUnited Statesen_US
dc.identifier.issnl1080-6040-

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