The effect of oral commensal bacteria on candidal adhesion to denture acrylic surfaces. An in vitro study

Abstract

The effect of four different species of oral bacteria (Streptococci (x2); Escherichia; Porphyromonas) on the adhesion of Candida albicans and Candida krusei to denture acrylic surfaces was studied using a previously described in vitro adhesion assay. Clear acrylic strips immersed in known concentrations of bacterial suspensions and incubated for 45 min to 1 h, at 37°C, were transferred to yeast suspensions of known concentrations and incubated for a similar period, and the adherent yeasts were quantified using an image analysis system. Pre-exposure to different bacterial concentrations resulted in suppression of candidal adhesion to varying degrees. Of three selected concentrations, exposure to the highest bacterial concentration (106 organisms/ml) resulted in a consistent reduction in candidal adhesion, except for E. coli-C. albicans and S. salivarius-C. albicans combinations. Candidal adhesion was significantly reduced on pre-exposure to S. sanguis and P. gingivalis, but not E. coli. There were also significant differences in relative adhesion between C. albicans and C. krusei. Taken together, these results indicate that the adhesion of yeasts to acrylic strips is modulated both by the quantity and the quality of pre-existing bacterial flora on acrylic surfaces.