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Article: Simple and convenient G-quadruplex-based turn-on fluorescence assay for 3′ → 5′ exonuclease activity

TitleSimple and convenient G-quadruplex-based turn-on fluorescence assay for 3′ → 5′ exonuclease activity
Authors
Leung, CHChan, DSHMan, BYWWang, CJLam, WCheng, YCFong, WFHsiao, WLWMa, DL
Issue Date2011
PublisherAmerican Chemical Society. The Journal's web site is located at http://pubs.acs.org/ac
Citation
Analytical Chemistry, 2011, v. 83 n. 2, p. 463-466 How to Cite?
DOI: http://dx.doi.org/10.1021/ac1025896
AbstractA selective, oligonucleotide-based, label-free, turn-on fluorescence detection method for 3′ → 5′ exonuclease activity has been developed using crystal violet as a G-quadruplex-binding probe. The assay is highly simple and rapid, does not require the use of gel-based equipment or radioisotopic labeling, and is amenable to high-throughput and real-time detection. A proof-of-concept of this assay has been demonstrated for prokaryotic Exonuclease III (ExoIII) and human TREX1. © 2010 American Chemical Society.
Persistent Identifierhttp://hdl.handle.net/10722/168503
ISSN
0003-2700
2021 Impact Factor: 8.008
2020 SCImago Journal Rankings: 2.117
ISI Accession Number ID
WOS:000286129800001
References
References in Scopus

 

DC FieldValueLanguage
dc.contributor.authorLeung, CHen_US
dc.contributor.authorChan, DSHen_US
dc.contributor.authorMan, BYWen_US
dc.contributor.authorWang, CJen_US
dc.contributor.authorLam, Wen_US
dc.contributor.authorCheng, YCen_US
dc.contributor.authorFong, WFen_US
dc.contributor.authorHsiao, WLWen_US
dc.contributor.authorMa, DLen_US
dc.date.accessioned2012-10-08T03:19:43Z-
dc.date.available2012-10-08T03:19:43Z-
dc.date.issued2011en_US
dc.identifier.citationAnalytical Chemistry, 2011, v. 83 n. 2, p. 463-466en_US
dc.identifier.issn0003-2700en_US
dc.identifier.urihttp://hdl.handle.net/10722/168503-
dc.description.abstractA selective, oligonucleotide-based, label-free, turn-on fluorescence detection method for 3′ → 5′ exonuclease activity has been developed using crystal violet as a G-quadruplex-binding probe. The assay is highly simple and rapid, does not require the use of gel-based equipment or radioisotopic labeling, and is amenable to high-throughput and real-time detection. A proof-of-concept of this assay has been demonstrated for prokaryotic Exonuclease III (ExoIII) and human TREX1. © 2010 American Chemical Society.en_US
dc.languageengen_US
dc.publisherAmerican Chemical Society. The Journal's web site is located at http://pubs.acs.org/acen_US
dc.relation.ispartofAnalytical Chemistryen_US
dc.subject.meshCircular Dichroismen_US
dc.subject.meshExodeoxyribonucleases - Metabolismen_US
dc.subject.meshExonucleases - Metabolismen_US
dc.subject.meshFluorescent Dyes - Chemistryen_US
dc.subject.meshG-Quadruplexesen_US
dc.subject.meshGentian Violet - Chemistryen_US
dc.subject.meshHumansen_US
dc.subject.meshOligonucleotides - Chemistry - Metabolismen_US
dc.subject.meshPhosphoproteins - Metabolismen_US
dc.subject.meshSpectrometry, Fluorescence - Methodsen_US
dc.titleSimple and convenient G-quadruplex-based turn-on fluorescence assay for 3′ → 5′ exonuclease activityen_US
dc.typeArticleen_US
dc.identifier.emailLeung, CH:duncanl@hkucc.hku.hken_US
dc.identifier.emailMa, DL:edmondma@hku.hken_US
dc.identifier.authorityLeung, CH=rp00730en_US
dc.identifier.authorityMa, DL=rp00760en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1021/ac1025896en_US
dc.identifier.pmid21114271-
dc.identifier.scopuseid_2-s2.0-78751558009en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-78751558009&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume83en_US
dc.identifier.issue2en_US
dc.identifier.spage463en_US
dc.identifier.epage466en_US
dc.identifier.isiWOS:000286129800001-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridLeung, CH=7402612570en_US
dc.identifier.scopusauthoridChan, DSH=35285471900en_US
dc.identifier.scopusauthoridMan, BYW=36652999800en_US
dc.identifier.scopusauthoridWang, CJ=35101184800en_US
dc.identifier.scopusauthoridLam, W=7203021943en_US
dc.identifier.scopusauthoridCheng, YC=36041844200en_US
dc.identifier.scopusauthoridFong, WF=7102816013en_US
dc.identifier.scopusauthoridHsiao, WLW=36795903400en_US
dc.identifier.scopusauthoridMa, DL=7402075538en_US
dc.identifier.citeulike8684312-
dc.identifier.issnl0003-2700-

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