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- Publisher Website: 10.1111/j.1748-1716.2010.02135.x
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- PMID: 20384597
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Article: Uridine adenosine tetraphosphate affects contractility of mouse aorta and decreases blood pressure in conscious rats and mice
Title | Uridine adenosine tetraphosphate affects contractility of mouse aorta and decreases blood pressure in conscious rats and mice |
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Authors | |
Keywords | blood pressure regulation dinucleotide glomerular filtration rate kidney function vascular reactivity |
Issue Date | 2010 |
Publisher | Wiley-Blackwell Publishing Ltd. The Journal's web site is located at http://www.wiley.com/bw/journal.asp?ref=1748-1708 |
Citation | Acta Physiologica, 2010, v. 200 n. 2, p. 171-179 How to Cite? |
Abstract | Aim: In the anaesthetized rat, uridine adenosine tetraphosphate (Up 4A) is a circulating, endothelium-derived vasoconstrictor presumably operating as such in un-anaesthetized animals. The present study investigated the in vivo effects of Up4A in conscious mice and rats, and its direct vascular effects in the mouse aorta in vitro. Methods: In vivo, Up 4A was given as step-up infusion at rates of 8-512 nmol min -1 kg-1 for 30 min periods in chronically catheterized rodents. In vitro, the effect of Up4A on rings of mouse aortae mounted in a myograph was tested. Results: High doses of Up4A (mice: 512 nmol min-1 kg-1; rats: 128 nmol min-1 kg-1) caused hypotension (99 ± 4 to 64 ± 7 mmHg and 114 ± 3 to 108 ± 3 mmHg, respectively, both P < 0.01). In rats, Up4A significantly decreased sodium excretion by >75% and potassium excretion by ∼60% without significant changes in urine flow. Exposure of phenylephrine-contracted rings to increasing concentrations of Up4A elicited contraction at 10-7 and 10-6 mol L-1 (18 ± 2% and 76 ± 16% respectively); unexpectedly, 10-5 mol L-1 caused a biphasic response with a contraction (19 ± 6%) followed by a relaxation (-46 ± 6%). No relaxation was observed when the concentration was increased further. Bolus exposure to 10 -5 mol L-1 of Up4A caused contraction (+80 ± 2%). Added successively to untreated vessels, increasing concentrations of Up4A (10-7-10-5 mol L-1) induced a biphasic response of contraction followed by relaxation. Conclusion: Up 4A has direct biphasic effects on vascular smooth muscle of the mouse aorta but vasoconstriction dominates at low concentrations. In conscious rodents, step-up infusions of Up4A elicit hypotension and electrolyte retention. © 2010 Scandinavian Physiological Society. |
Persistent Identifier | http://hdl.handle.net/10722/171406 |
ISSN | 2021 Impact Factor: 7.523 2020 SCImago Journal Rankings: 1.591 |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Hansen, PB | en_US |
dc.contributor.author | Hristovska, A | en_US |
dc.contributor.author | Wolff, H | en_US |
dc.contributor.author | Vanhoutte, P | en_US |
dc.contributor.author | Jensen, BL | en_US |
dc.contributor.author | Bie, P | en_US |
dc.date.accessioned | 2012-10-30T06:13:57Z | - |
dc.date.available | 2012-10-30T06:13:57Z | - |
dc.date.issued | 2010 | en_US |
dc.identifier.citation | Acta Physiologica, 2010, v. 200 n. 2, p. 171-179 | en_US |
dc.identifier.issn | 1748-1708 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/171406 | - |
dc.description.abstract | Aim: In the anaesthetized rat, uridine adenosine tetraphosphate (Up 4A) is a circulating, endothelium-derived vasoconstrictor presumably operating as such in un-anaesthetized animals. The present study investigated the in vivo effects of Up4A in conscious mice and rats, and its direct vascular effects in the mouse aorta in vitro. Methods: In vivo, Up 4A was given as step-up infusion at rates of 8-512 nmol min -1 kg-1 for 30 min periods in chronically catheterized rodents. In vitro, the effect of Up4A on rings of mouse aortae mounted in a myograph was tested. Results: High doses of Up4A (mice: 512 nmol min-1 kg-1; rats: 128 nmol min-1 kg-1) caused hypotension (99 ± 4 to 64 ± 7 mmHg and 114 ± 3 to 108 ± 3 mmHg, respectively, both P < 0.01). In rats, Up4A significantly decreased sodium excretion by >75% and potassium excretion by ∼60% without significant changes in urine flow. Exposure of phenylephrine-contracted rings to increasing concentrations of Up4A elicited contraction at 10-7 and 10-6 mol L-1 (18 ± 2% and 76 ± 16% respectively); unexpectedly, 10-5 mol L-1 caused a biphasic response with a contraction (19 ± 6%) followed by a relaxation (-46 ± 6%). No relaxation was observed when the concentration was increased further. Bolus exposure to 10 -5 mol L-1 of Up4A caused contraction (+80 ± 2%). Added successively to untreated vessels, increasing concentrations of Up4A (10-7-10-5 mol L-1) induced a biphasic response of contraction followed by relaxation. Conclusion: Up 4A has direct biphasic effects on vascular smooth muscle of the mouse aorta but vasoconstriction dominates at low concentrations. In conscious rodents, step-up infusions of Up4A elicit hypotension and electrolyte retention. © 2010 Scandinavian Physiological Society. | en_US |
dc.language | eng | en_US |
dc.publisher | Wiley-Blackwell Publishing Ltd. The Journal's web site is located at http://www.wiley.com/bw/journal.asp?ref=1748-1708 | en_US |
dc.relation.ispartof | Acta Physiologica | en_US |
dc.subject | blood pressure regulation | - |
dc.subject | dinucleotide | - |
dc.subject | glomerular filtration rate | - |
dc.subject | kidney function | - |
dc.subject | vascular reactivity | - |
dc.subject.mesh | Animals | en_US |
dc.subject.mesh | Aorta - Drug Effects - Physiology | en_US |
dc.subject.mesh | Blood Pressure - Drug Effects - Physiology | en_US |
dc.subject.mesh | Consciousness | en_US |
dc.subject.mesh | Dinucleoside Phosphates - Metabolism - Pharmacology | en_US |
dc.subject.mesh | Female | en_US |
dc.subject.mesh | Kidney - Physiology | en_US |
dc.subject.mesh | Kidney Function Tests | en_US |
dc.subject.mesh | Male | en_US |
dc.subject.mesh | Mice | en_US |
dc.subject.mesh | Mice, Inbred C57bl | en_US |
dc.subject.mesh | Muscle Contraction - Drug Effects - Physiology | en_US |
dc.subject.mesh | Muscle, Smooth, Vascular - Drug Effects - Physiology | en_US |
dc.subject.mesh | Rats | en_US |
dc.subject.mesh | Rats, Sprague-Dawley | en_US |
dc.subject.mesh | Vasoconstriction - Drug Effects - Physiology | en_US |
dc.title | Uridine adenosine tetraphosphate affects contractility of mouse aorta and decreases blood pressure in conscious rats and mice | en_US |
dc.type | Article | en_US |
dc.identifier.email | Vanhoutte, P:vanhoutt@hku.hk | en_US |
dc.identifier.authority | Vanhoutte, P=rp00238 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.doi | 10.1111/j.1748-1716.2010.02135.x | en_US |
dc.identifier.pmid | 20384597 | - |
dc.identifier.scopus | eid_2-s2.0-77956308849 | en_US |
dc.identifier.hkuros | 183720 | - |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-77956308849&selection=ref&src=s&origin=recordpage | en_US |
dc.identifier.volume | 200 | en_US |
dc.identifier.issue | 2 | en_US |
dc.identifier.spage | 171 | en_US |
dc.identifier.epage | 179 | en_US |
dc.identifier.isi | WOS:000281557700006 | - |
dc.publisher.place | United Kingdom | en_US |
dc.identifier.scopusauthorid | Hansen, PB=35472646600 | en_US |
dc.identifier.scopusauthorid | Hristovska, A=19933493100 | en_US |
dc.identifier.scopusauthorid | Wolff, H=23994503400 | en_US |
dc.identifier.scopusauthorid | Vanhoutte, P=7202304247 | en_US |
dc.identifier.scopusauthorid | Jensen, BL=35502338900 | en_US |
dc.identifier.scopusauthorid | Bie, P=7006398859 | en_US |
dc.identifier.citeulike | 7857419 | - |
dc.identifier.issnl | 1748-1708 | - |