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Article: Lack of growth hormone-releasing peptide-6 action on in vivo and in vitro growth hormone secretion in sexually immature and grass carp (Ctenopharyngodon idellus)
Title | Lack of growth hormone-releasing peptide-6 action on in vivo and in vitro growth hormone secretion in sexually immature and grass carp (Ctenopharyngodon idellus) |
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Authors | |
Keywords | Growth Hormone Growth Hormone-Releasing Peptide-6 Hexarelin Juvenile Grass Carp Perifusion Pituitary Cells Pituitary Fragments Static Incubation |
Issue Date | 2002 |
Publisher | Springer Verlag Dordrecht. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0920-1742 |
Citation | Fish Physiology And Biochemistry, 2002, v. 26 n. 4, p. 315-327 How to Cite? |
Abstract | Studies in mammals have shown that synthetic Met-enkephalin derivatives, called growth hormone-releasing peptides (GHRPs), stimulate growth hormone (GH) release. In the present study, GHRP-6 action on GH secretion was examined in vivo and in vitro in sexually immature grass carp. GHRP-6 injected intraperitoneally had no influences on serum GH levels in juvenile grass carp. Following intraperitonal injection of GHRP-6 and dopamine (DA) or cysteamine hydrochloride (CSH), alone and in combination into juvenile grass carp, DA and CSH were effective in elevating serum GH levels, but GHRP-6 was not effective in this respect; in addition, the synergistic action of GHRP-6 and DA or CSH on GH secretion was not seen. In this work, we had adapted and validated a perifusion system and a culture system for GH regulation studies. In a perifusion system, GHRP-6 (1000 to 0.1 nM), GHRP-6 (0.1 to 1000 nM), GHRP-6 (1 μM), and Hexarelin (an analog of GHRP, 1 μM) had no action on GH release from juvenile grass carp pituitary fragments or cells. Under static incubation conditions, GHRP-6 was inactive on GH release from juvenile grass carp pituitary fragments after 1 h and 6 h incubation, but human growth hormone-releasing hormone (hGHRH; 1 to 100 nM) as positive control could stimulate GH release in a dose-dependent manner. Furthermore, when GHRP-6 (100 nM) in combination static incubation with neuropeptides [e.g., hGHRH (100 nM), salmon gonadotropin-releasing hormone analogue (sGnRH-A) (100 nM), or D-Ala 6,Pro 9- NEt-luteinizing hormone-releasing hormone (D-Ala 6,Pro 6-NEt-LHRH, LHRH-A) (100nM)], GHRP-6 did not strengthen GH secretion actions of neuropeptides, and at the same time neuropeptides also did not modify the effects of GHRP-6 on GH secretion. The present results obtained using in vivo and in vitro techniques adapted for GH regulation studies show that GHRP-6 does not function as a GH-releasing factor in juvenile grass carp as it does in tilapia, amphibians, chickens, and mammals. |
Persistent Identifier | http://hdl.handle.net/10722/178872 |
ISSN | 2021 Impact Factor: 3.014 2020 SCImago Journal Rankings: 0.680 |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
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dc.contributor.author | Xiao, D | en_US |
dc.contributor.author | Wong, AOL | en_US |
dc.contributor.author | Lin, HR | en_US |
dc.date.accessioned | 2012-12-19T09:50:17Z | - |
dc.date.available | 2012-12-19T09:50:17Z | - |
dc.date.issued | 2002 | en_US |
dc.identifier.citation | Fish Physiology And Biochemistry, 2002, v. 26 n. 4, p. 315-327 | en_US |
dc.identifier.issn | 0920-1742 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/178872 | - |
dc.description.abstract | Studies in mammals have shown that synthetic Met-enkephalin derivatives, called growth hormone-releasing peptides (GHRPs), stimulate growth hormone (GH) release. In the present study, GHRP-6 action on GH secretion was examined in vivo and in vitro in sexually immature grass carp. GHRP-6 injected intraperitoneally had no influences on serum GH levels in juvenile grass carp. Following intraperitonal injection of GHRP-6 and dopamine (DA) or cysteamine hydrochloride (CSH), alone and in combination into juvenile grass carp, DA and CSH were effective in elevating serum GH levels, but GHRP-6 was not effective in this respect; in addition, the synergistic action of GHRP-6 and DA or CSH on GH secretion was not seen. In this work, we had adapted and validated a perifusion system and a culture system for GH regulation studies. In a perifusion system, GHRP-6 (1000 to 0.1 nM), GHRP-6 (0.1 to 1000 nM), GHRP-6 (1 μM), and Hexarelin (an analog of GHRP, 1 μM) had no action on GH release from juvenile grass carp pituitary fragments or cells. Under static incubation conditions, GHRP-6 was inactive on GH release from juvenile grass carp pituitary fragments after 1 h and 6 h incubation, but human growth hormone-releasing hormone (hGHRH; 1 to 100 nM) as positive control could stimulate GH release in a dose-dependent manner. Furthermore, when GHRP-6 (100 nM) in combination static incubation with neuropeptides [e.g., hGHRH (100 nM), salmon gonadotropin-releasing hormone analogue (sGnRH-A) (100 nM), or D-Ala 6,Pro 9- NEt-luteinizing hormone-releasing hormone (D-Ala 6,Pro 6-NEt-LHRH, LHRH-A) (100nM)], GHRP-6 did not strengthen GH secretion actions of neuropeptides, and at the same time neuropeptides also did not modify the effects of GHRP-6 on GH secretion. The present results obtained using in vivo and in vitro techniques adapted for GH regulation studies show that GHRP-6 does not function as a GH-releasing factor in juvenile grass carp as it does in tilapia, amphibians, chickens, and mammals. | en_US |
dc.language | eng | en_US |
dc.publisher | Springer Verlag Dordrecht. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0920-1742 | en_US |
dc.relation.ispartof | Fish Physiology and Biochemistry | en_US |
dc.subject | Growth Hormone | en_US |
dc.subject | Growth Hormone-Releasing Peptide-6 | en_US |
dc.subject | Hexarelin | en_US |
dc.subject | Juvenile Grass Carp | en_US |
dc.subject | Perifusion | en_US |
dc.subject | Pituitary Cells | en_US |
dc.subject | Pituitary Fragments | en_US |
dc.subject | Static Incubation | en_US |
dc.title | Lack of growth hormone-releasing peptide-6 action on in vivo and in vitro growth hormone secretion in sexually immature and grass carp (Ctenopharyngodon idellus) | en_US |
dc.type | Article | en_US |
dc.identifier.email | Wong, AOL: olwong@hkucc.hku.hk | en_US |
dc.identifier.authority | Wong, AOL=rp00806 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.doi | 10.1023/B:FISH.0000009260.34930.12 | en_US |
dc.identifier.scopus | eid_2-s2.0-1642558448 | en_US |
dc.identifier.hkuros | 113616 | - |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-1642558448&selection=ref&src=s&origin=recordpage | en_US |
dc.identifier.volume | 26 | en_US |
dc.identifier.issue | 4 | en_US |
dc.identifier.spage | 315 | en_US |
dc.identifier.epage | 327 | en_US |
dc.identifier.isi | WOS:000187354100002 | - |
dc.publisher.place | Netherlands | en_US |
dc.identifier.scopusauthorid | Xiao, D=45561966600 | en_US |
dc.identifier.scopusauthorid | Wong, AOL=7403147570 | en_US |
dc.identifier.scopusauthorid | Lin, HR=7405571313 | en_US |
dc.identifier.issnl | 0920-1742 | - |