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Article: Combination of high-resolution magic angle spinning proton magnetic resonance spectroscopy and microscale genomics to type brain tumor biopsies
Title | Combination of high-resolution magic angle spinning proton magnetic resonance spectroscopy and microscale genomics to type brain tumor biopsies |
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Authors | |
Keywords | Anaplastic Ganglioglioma Brain/Cns Cancers Ex Vivo High-Resolution Magic Angle Spinning Magnetic Resonance Spectroscopy Genomics Tumor Biomarkers |
Issue Date | 2007 |
Publisher | Demetrios A Spandidos Ed & Pub. The Journal's web site is located at http://147.52.72.117/IJMM/ijmm.htm |
Citation | International Journal Of Molecular Medicine, 2007, v. 20 n. 2, p. 199-208 How to Cite? |
Abstract | Advancements in the diagnosis and prognosis of brain tumor patients, and thus in their survival and quality of life, can be achieved using biomarkers that facilitate improved tumor typing. We introduce and implement a combinatorial metabolic and molecular approach that applies state-of-the-art, high-resolution magic angle spinning (HRMAS) proton ( 1H) MRS and gene transcriptome profiling to intact brain tumor biopsies, to identify unique biomarker profiles of brain tumors. Our results show that samples as small as 2 mg can be successfully processed, the HRMAS 1H MRS procedure does not result in mRNA degradation, and minute mRNA amounts yield high-quality genomic data. The MRS and genomic analyses demonstrate that CNS tumors have altered levels of specific 1H MRS metabolites that directly correspond to altered expression of Kennedy pathway genes; and exhibit rapid phospholipid turnover, which coincides with upregulation of cell proliferation genes. The data also suggest Sonic Hedgehog pathway (SHH) dysregulation may play a role in anaplastic ganglioglioma pathogenesis. That a strong correlation is seen between the HRMAS 1H MRS and genomic data cross-validates and further demonstrates the biological relevance of the MRS results. Our combined metabolic/molecular MRS/genomic approach provides insights into the biology of anaplastic ganglioglioma and a new potential tumor typing methodology that could aid neurologists and neurosurgeons to improve the diagnosis, treatment, and ongoing evaluation of brain tumor patients. |
Persistent Identifier | http://hdl.handle.net/10722/180729 |
ISSN | 2021 Impact Factor: 5.314 2020 SCImago Journal Rankings: 1.048 |
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Tzika, AA | en_US |
dc.contributor.author | Astrakas, L | en_US |
dc.contributor.author | Cao, H | en_US |
dc.contributor.author | Mintzopoulos, D | en_US |
dc.contributor.author | Andronesi, OC | en_US |
dc.contributor.author | Mindrinos, M | en_US |
dc.contributor.author | Zhang, J | en_US |
dc.contributor.author | Rahme, LG | en_US |
dc.contributor.author | Blekas, KD | en_US |
dc.contributor.author | Likas, AC | en_US |
dc.contributor.author | Galatsanos, NP | en_US |
dc.contributor.author | Carroll, RS | en_US |
dc.contributor.author | Black, PM | en_US |
dc.date.accessioned | 2013-01-28T01:42:04Z | - |
dc.date.available | 2013-01-28T01:42:04Z | - |
dc.date.issued | 2007 | en_US |
dc.identifier.citation | International Journal Of Molecular Medicine, 2007, v. 20 n. 2, p. 199-208 | en_US |
dc.identifier.issn | 1107-3756 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/180729 | - |
dc.description.abstract | Advancements in the diagnosis and prognosis of brain tumor patients, and thus in their survival and quality of life, can be achieved using biomarkers that facilitate improved tumor typing. We introduce and implement a combinatorial metabolic and molecular approach that applies state-of-the-art, high-resolution magic angle spinning (HRMAS) proton ( 1H) MRS and gene transcriptome profiling to intact brain tumor biopsies, to identify unique biomarker profiles of brain tumors. Our results show that samples as small as 2 mg can be successfully processed, the HRMAS 1H MRS procedure does not result in mRNA degradation, and minute mRNA amounts yield high-quality genomic data. The MRS and genomic analyses demonstrate that CNS tumors have altered levels of specific 1H MRS metabolites that directly correspond to altered expression of Kennedy pathway genes; and exhibit rapid phospholipid turnover, which coincides with upregulation of cell proliferation genes. The data also suggest Sonic Hedgehog pathway (SHH) dysregulation may play a role in anaplastic ganglioglioma pathogenesis. That a strong correlation is seen between the HRMAS 1H MRS and genomic data cross-validates and further demonstrates the biological relevance of the MRS results. Our combined metabolic/molecular MRS/genomic approach provides insights into the biology of anaplastic ganglioglioma and a new potential tumor typing methodology that could aid neurologists and neurosurgeons to improve the diagnosis, treatment, and ongoing evaluation of brain tumor patients. | en_US |
dc.language | eng | en_US |
dc.publisher | Demetrios A Spandidos Ed & Pub. The Journal's web site is located at http://147.52.72.117/IJMM/ijmm.htm | en_US |
dc.relation.ispartof | International Journal of Molecular Medicine | en_US |
dc.subject | Anaplastic Ganglioglioma | en_US |
dc.subject | Brain/Cns Cancers | en_US |
dc.subject | Ex Vivo High-Resolution Magic Angle Spinning Magnetic Resonance Spectroscopy | en_US |
dc.subject | Genomics | en_US |
dc.subject | Tumor Biomarkers | en_US |
dc.title | Combination of high-resolution magic angle spinning proton magnetic resonance spectroscopy and microscale genomics to type brain tumor biopsies | en_US |
dc.type | Article | en_US |
dc.identifier.email | Zhang, J: jzhang1@hku.hk | en_US |
dc.identifier.authority | Zhang, J=rp01713 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.scopus | eid_2-s2.0-35448970283 | en_US |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-35448970283&selection=ref&src=s&origin=recordpage | en_US |
dc.identifier.volume | 20 | en_US |
dc.identifier.issue | 2 | en_US |
dc.identifier.spage | 199 | en_US |
dc.identifier.epage | 208 | en_US |
dc.publisher.place | Greece | en_US |
dc.identifier.scopusauthorid | Tzika, AA=7003635500 | en_US |
dc.identifier.scopusauthorid | Astrakas, L=6603155897 | en_US |
dc.identifier.scopusauthorid | Cao, H=36829765800 | en_US |
dc.identifier.scopusauthorid | Mintzopoulos, D=16070342600 | en_US |
dc.identifier.scopusauthorid | Andronesi, OC=6506599452 | en_US |
dc.identifier.scopusauthorid | Mindrinos, M=6603048545 | en_US |
dc.identifier.scopusauthorid | Zhang, J=22137260600 | en_US |
dc.identifier.scopusauthorid | Rahme, LG=6603919311 | en_US |
dc.identifier.scopusauthorid | Blekas, KD=6603665613 | en_US |
dc.identifier.scopusauthorid | Likas, AC=7003844484 | en_US |
dc.identifier.scopusauthorid | Galatsanos, NP=35562970900 | en_US |
dc.identifier.scopusauthorid | Carroll, RS=7201449106 | en_US |
dc.identifier.scopusauthorid | Black, PM=17633338700 | en_US |
dc.identifier.issnl | 1107-3756 | - |