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Article: Impact of direct cell co-cultures on human adipose-derived stromal cells and nucleus pulposus cells

TitleImpact of direct cell co-cultures on human adipose-derived stromal cells and nucleus pulposus cells
Authors
Keywordsadipose-derived stem cells
direct co-culture
intervertebral disc degeneration
nucleus pulposus
Issue Date2013
PublisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://onlinelibrary.wiley.com/journal/10.1002/(ISSN)1554-527X
Citation
Journal of Orthopaedic Research, 2013, v. 31 n. 11, p. 1804-1813 How to Cite?
AbstractBiologic and cellular treatment strategies aiming for curing intervertebral disc degeneration (IDD) have been proposed recently. Given the convenient availability and expansion potential, adipose-derived stromal cells (ADSCs) might be an ideal cell candidate. However, the interaction between ADSCs and nucleus pulposus (NP) cells still remains ambiguous, especially in direct co-cultures of the two types of cells. Nevertheless, NP markers in ADSCs after co-cultures were unidentified. Here, we addressed the interaction of human ADSCs and NP cells in a direct co-culture system for the first time. As a result, ADSCs could differentiate to the NP cell phenotype with a significant up-regulated expression of multiple genes and proteins in extracellular matrix (ECM) (SOX9, COL2A1, ACAN, and COL6A2), relative NP markers (FOXF1, PAX1, CA12, and HBB) and pertinent growth factors (CDMP-1, TGF-beta1, IGF-1, and CTGF). Moreover, the gene expression of COL2A1, ACAN, and COL6A2 of degenerate NP cells was also up-regulated. Collectively, these results suggest that direct co-cultures of ADSCs and NP cells may exert a reciprocal impact, that is, both stimulating ADSCs differentiation to the NP cell phenotype and inducing NP cells to regain functional phenotype. Accordingly, ADSCs might be a potential candidate in the development of cellular treatment strategies for IDD.
Persistent Identifierhttp://hdl.handle.net/10722/203244
ISSN
2019 Impact Factor: 2.728
2015 SCImago Journal Rankings: 1.464
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorSun, Z-
dc.contributor.authorLiu, ZH-
dc.contributor.authorZhao, XH-
dc.contributor.authorSun, L-
dc.contributor.authorChen, YF-
dc.contributor.authorZhang, WL-
dc.contributor.authorGao, Y-
dc.contributor.authorZhang, YZ-
dc.contributor.authorWan, ZY-
dc.contributor.authorSamartzis, D-
dc.contributor.authorWang, HQ-
dc.contributor.authorLuo, ZJ-
dc.date.accessioned2014-09-19T13:11:30Z-
dc.date.available2014-09-19T13:11:30Z-
dc.date.issued2013-
dc.identifier.citationJournal of Orthopaedic Research, 2013, v. 31 n. 11, p. 1804-1813-
dc.identifier.issn0736-0266-
dc.identifier.urihttp://hdl.handle.net/10722/203244-
dc.description.abstractBiologic and cellular treatment strategies aiming for curing intervertebral disc degeneration (IDD) have been proposed recently. Given the convenient availability and expansion potential, adipose-derived stromal cells (ADSCs) might be an ideal cell candidate. However, the interaction between ADSCs and nucleus pulposus (NP) cells still remains ambiguous, especially in direct co-cultures of the two types of cells. Nevertheless, NP markers in ADSCs after co-cultures were unidentified. Here, we addressed the interaction of human ADSCs and NP cells in a direct co-culture system for the first time. As a result, ADSCs could differentiate to the NP cell phenotype with a significant up-regulated expression of multiple genes and proteins in extracellular matrix (ECM) (SOX9, COL2A1, ACAN, and COL6A2), relative NP markers (FOXF1, PAX1, CA12, and HBB) and pertinent growth factors (CDMP-1, TGF-beta1, IGF-1, and CTGF). Moreover, the gene expression of COL2A1, ACAN, and COL6A2 of degenerate NP cells was also up-regulated. Collectively, these results suggest that direct co-cultures of ADSCs and NP cells may exert a reciprocal impact, that is, both stimulating ADSCs differentiation to the NP cell phenotype and inducing NP cells to regain functional phenotype. Accordingly, ADSCs might be a potential candidate in the development of cellular treatment strategies for IDD.-
dc.languageeng-
dc.publisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://onlinelibrary.wiley.com/journal/10.1002/(ISSN)1554-527X-
dc.relation.ispartofJournal of Orthopaedic Research-
dc.subjectadipose-derived stem cells-
dc.subjectdirect co-culture-
dc.subjectintervertebral disc degeneration-
dc.subjectnucleus pulposus-
dc.titleImpact of direct cell co-cultures on human adipose-derived stromal cells and nucleus pulposus cells-
dc.typeArticle-
dc.identifier.emailSamartzis, D: dspine@hku.hk-
dc.identifier.authoritySamartzis, D=rp01430-
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1002/jor.22439-
dc.identifier.pmid23913869-
dc.identifier.scopuseid_2-s2.0-84884910557-
dc.identifier.hkuros237999-
dc.identifier.volume31-
dc.identifier.issue11-
dc.identifier.spage1804-
dc.identifier.epage1813-
dc.identifier.isiWOS:000324930500019-
dc.publisher.placeUnited States-

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