Lipid subclasses profiles and oxidative stress in aggressive periodontitis before and after treatment

Abstract

OBJECTIVES: The aim of this study was to investigate the lipid and oxidative stress profiles in aggressive periodontitis (AgP) patients, and to relate them to clinical variables and IL-6 genetic variants. METHODS: Twelve non-smoking Caucasian AgP patients selected based on their IL6 haplotypes underwent periodontal non-surgical and surgical treatment. Peripheral blood samples taken at baseline and at 6 different time-points after treatment were processed to determine lipid profiles (cholesterol, triglycerides, HDL and LDL subclasses) and oxidative stress markers (glutathione and total lipid hydroperoxide levels).RESULTS: HDL were the highest represented lipoproteins (38%), followed by IDL (26%), VLDL (20%), and LDL (16%). Among LDL, there was a prevalence of large LDL (LDL-1 and LDL-2), with a very small prevalence of small LDL. The lipid profile did not consistently change after treatment up to 3 months after surgery. Periodontal disease severity was associated with changes in concentrations of LDL (p=0.01), large IDL (p=0.04), medium IDL (p=0.004), small IDL (p<0.001), LDL-3 and LDL-2 and LDL-3 (p<0.001) and average LDL size (p<0.001). The IL6 haplotypes were associated with total cholesterol, triglycerides, HDL and LDL subclasses after adjusting for confounders (p<0.01). No associations were detected between clinical and genetic variables and oxidative stress parameters. CONCLUSIONS: Disease severity and IL6 haplotypes may influence lipid profiles in aggressive periodontitis.