Development of recombinant adeno-associated virus-mediated delivery adiponectin in mice

Abstract

Obesity is a global health problem experienced with various kinds of metabolic syndromes such as insulin resistance, hyperlipidemia and hypoadiponectinemia. To ameliorate obesity related problems, attention has been drawn to explore the role of adiponectin in obesity related metabolic dysregulation such that it can be selected as a therapeutic target to improve obesity related health problems. There are tools to over-express adiponectin such as drug induced secretion and osmotic pump drug delivery system for basic research. In this project, recombinant adeno-associated virus serotype 2/8 (rAAV2/8-LSP-mADN) was used to overexpress adiponectin in the liver of mouse for basic and translational research studies. Western blot of hepatocyte lysate and serum against adiponectin as well as hepatocyte viral quantitative PCR data suggests successful hepatic transduction. Although the expression level of adiponectin by this system need to further optimize, rAAV2/8-LSP-mADN is a valuable tool to over-express adiponectin in vivo safely and effectively.