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Article: In vivo periodontal tissue regeneration by periodontal ligament stem cells and endothelial cells in three-dimensional cell sheet constructs

TitleIn vivo periodontal tissue regeneration by periodontal ligament stem cells and endothelial cells in three-dimensional cell sheet constructs
Authors
KeywordsTissue engineering
Periodontal ligament stem cells
Endothelial cells
Cell sheet
Issue Date2017
PublisherWiley-Blackwell Publishing, Inc.. The Journal's web site is located at http://www.wiley.com/bw/journal.asp?ref=0022-3484&site=1
Citation
Journal of Periodontal Research, 2017, v. 52, p. 408-418 How to Cite?
Abstract© 2016 John Wiley & Sons A/S.Background and Objective: Chronic periodontitis causes damage to tooth-supporting tissues, resulting in tooth loss in adults. Recently, cell-sheet-based approaches have been studied to overcome the limitations of conventional cytotherapeutic procedures for periodontal regeneration. The purpose of the present study was to investigate the regenerative potential of periodontal ligament stem cells (PDLSCs) and human umbilical vein endothelial cells (HUVECs) in three-dimensional (3D) cell sheet constructs for periodontal regeneration in vivo. Material and Methods: PDLSCs, HUVECs or co-cultures of both cells were seeded onto temperature-responsive culture dishes, and intact cell sheets were fabricated. Cell sheets were wrapped around the prepared human roots in three different combinations and implanted subcutaneously into immunodeficient mice. Results: Histological evaluation revealed that after 2, 4 and 8 wk of implantation, periodontal ligament-like tissue arrangements were observed around the implanted roots in experimental groups compared with controls. Vascular lumens were also observed in periodontal compartments of HUVEC-containing groups. Periodontal ligament regeneration, cementogenesis and osteogenesis were evident in the experimental groups at both weeks 4 and 8, as shown by immunostaining for periostin and bone sialoprotein. Human cells in the transplanted cell sheets were stained by immunohistochemistry for the presence of human mitochondria. Conclusions: The 3D cell sheet-based approach may be potentially beneficial and is thus encouraged for future regenerative periodontal therapy.
Persistent Identifierhttp://hdl.handle.net/10722/236080
ISSN
2019 Impact Factor: 2.926
2015 SCImago Journal Rankings: 0.932
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorPanduwawala Kankanamalage, CPP-
dc.contributor.authorZhan, X-
dc.contributor.authorDissanayaka, WL-
dc.contributor.authorSamaranayake, LP-
dc.contributor.authorJin, L-
dc.contributor.authorZhang, C-
dc.date.accessioned2016-11-11T07:42:53Z-
dc.date.available2016-11-11T07:42:53Z-
dc.date.issued2017-
dc.identifier.citationJournal of Periodontal Research, 2017, v. 52, p. 408-418-
dc.identifier.issn0022-3484-
dc.identifier.urihttp://hdl.handle.net/10722/236080-
dc.description.abstract© 2016 John Wiley & Sons A/S.Background and Objective: Chronic periodontitis causes damage to tooth-supporting tissues, resulting in tooth loss in adults. Recently, cell-sheet-based approaches have been studied to overcome the limitations of conventional cytotherapeutic procedures for periodontal regeneration. The purpose of the present study was to investigate the regenerative potential of periodontal ligament stem cells (PDLSCs) and human umbilical vein endothelial cells (HUVECs) in three-dimensional (3D) cell sheet constructs for periodontal regeneration in vivo. Material and Methods: PDLSCs, HUVECs or co-cultures of both cells were seeded onto temperature-responsive culture dishes, and intact cell sheets were fabricated. Cell sheets were wrapped around the prepared human roots in three different combinations and implanted subcutaneously into immunodeficient mice. Results: Histological evaluation revealed that after 2, 4 and 8 wk of implantation, periodontal ligament-like tissue arrangements were observed around the implanted roots in experimental groups compared with controls. Vascular lumens were also observed in periodontal compartments of HUVEC-containing groups. Periodontal ligament regeneration, cementogenesis and osteogenesis were evident in the experimental groups at both weeks 4 and 8, as shown by immunostaining for periostin and bone sialoprotein. Human cells in the transplanted cell sheets were stained by immunohistochemistry for the presence of human mitochondria. Conclusions: The 3D cell sheet-based approach may be potentially beneficial and is thus encouraged for future regenerative periodontal therapy.-
dc.languageeng-
dc.publisherWiley-Blackwell Publishing, Inc.. The Journal's web site is located at http://www.wiley.com/bw/journal.asp?ref=0022-3484&site=1-
dc.relation.ispartofJournal of Periodontal Research-
dc.rightsPreprint This is the pre-peer reviewed version of the following article: [FULL CITE], which has been published in final form at [Link to final article]. Authors are not required to remove preprints posted prior to acceptance of the submitted version. Postprint This is the accepted version of the following article: [full citation], which has been published in final form at [Link to final article].-
dc.subjectTissue engineering-
dc.subjectPeriodontal ligament stem cells-
dc.subjectEndothelial cells-
dc.subjectCell sheet-
dc.titleIn vivo periodontal tissue regeneration by periodontal ligament stem cells and endothelial cells in three-dimensional cell sheet constructs-
dc.typeArticle-
dc.identifier.emailPanduwawala Kankanamalage, CPP: pandu@hku.hk-
dc.identifier.emailDissanayaka, WL: warunad@hku.hk-
dc.identifier.emailJin, L: ljjin@hkucc.hku.hk-
dc.identifier.emailZhang, C: zhangcf@hku.hk-
dc.identifier.authorityDissanayaka, WL=rp02216-
dc.identifier.authorityJin, L=rp00028-
dc.identifier.authorityZhang, C=rp01408-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1111/jre.12405-
dc.identifier.scopuseid_2-s2.0-84982860060-
dc.identifier.hkuros278266-
dc.identifier.hkuros270770-
dc.identifier.volume52-
dc.identifier.spage408-
dc.identifier.epage418-
dc.identifier.eissn1600-0765-
dc.identifier.isiWOS:000399955500012-
dc.publisher.placeUnited States-

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