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Article: Dual role of insulin in spexin regulation: Functional link between food intake and spexin expression in fish model

TitleDual role of insulin in spexin regulation: Functional link between food intake and spexin expression in fish model
Authors
Issue Date2017
PublisherOxford University Press. The Journal's web site is located at http://endo.endojournals.org
Citation
Endocrinology, 2017, v. 158 n. 3, p. 560-577 How to Cite?
AbstractSpexin (SPX), a neuropeptide discovered by the bioinformatics approach, has been recently identified as a satiety factor in a fish model. However, the functional link between feeding and SPX expression as well as the signal transduction for SPX regulation are totally unknown. In this study, we used goldfish as a model to examine the functional role of insulin as a postprandial signal for SPX regulation in bony fish. In goldfish, feeding could elevate plasma levels of glucose, insulin, and SPX with concurrent rises in insulin and SPX messenger RNA (mRNA) expression in the liver. Similar elevation in SPX mRNA level was also observed in the liver and brain areas involved in appetite control in goldfish after intraperitoneal injection of glucose and insulin, respectively. In parallel experiments with goldfish hepatocytes and brain cell culture, insulin signal induced by glucose was shown to exert a dual role in SPX regulation, namely (1) acting as an autocrine/paracrine signal to trigger SPX mRNA expression in the liver and (2) serving as an endocrine signal to induce SPX gene expression in the brain. Apparently, the peripheral (in the liver) and central actions of insulin (in the brain) on SPX gene expression were mediated by insulin receptor (to a lesser extent by insulin-like growth factor I receptor) coupled to mitogen-activated protein kinase kinase 3/6/p38 mitogen-activated protein kinase and phosphatidylinositol 3-kinase/Akt/mammalian target of rapamycin but not mitogen-activated protein kinase kinase 1/2/extracellular signal-regulated kinase 1/2 cascades. Our findings indicate that an insulin component inducible by glucose is present in the liver of the fish model and may serve as the postprandial signal linking food intake with SPX expression both in the central as well as at the hepatic level.
Persistent Identifierhttp://hdl.handle.net/10722/248716
ISSN
2019 Impact Factor: 3.934
2015 SCImago Journal Rankings: 2.363
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorMa, A-
dc.contributor.authorHe, M-
dc.contributor.authorBai, J-
dc.contributor.authorWong, MKH-
dc.contributor.authorKo, WKW-
dc.contributor.authorWong, AOL-
dc.date.accessioned2017-10-18T08:47:25Z-
dc.date.available2017-10-18T08:47:25Z-
dc.date.issued2017-
dc.identifier.citationEndocrinology, 2017, v. 158 n. 3, p. 560-577-
dc.identifier.issn0013-7227-
dc.identifier.urihttp://hdl.handle.net/10722/248716-
dc.description.abstractSpexin (SPX), a neuropeptide discovered by the bioinformatics approach, has been recently identified as a satiety factor in a fish model. However, the functional link between feeding and SPX expression as well as the signal transduction for SPX regulation are totally unknown. In this study, we used goldfish as a model to examine the functional role of insulin as a postprandial signal for SPX regulation in bony fish. In goldfish, feeding could elevate plasma levels of glucose, insulin, and SPX with concurrent rises in insulin and SPX messenger RNA (mRNA) expression in the liver. Similar elevation in SPX mRNA level was also observed in the liver and brain areas involved in appetite control in goldfish after intraperitoneal injection of glucose and insulin, respectively. In parallel experiments with goldfish hepatocytes and brain cell culture, insulin signal induced by glucose was shown to exert a dual role in SPX regulation, namely (1) acting as an autocrine/paracrine signal to trigger SPX mRNA expression in the liver and (2) serving as an endocrine signal to induce SPX gene expression in the brain. Apparently, the peripheral (in the liver) and central actions of insulin (in the brain) on SPX gene expression were mediated by insulin receptor (to a lesser extent by insulin-like growth factor I receptor) coupled to mitogen-activated protein kinase kinase 3/6/p38 mitogen-activated protein kinase and phosphatidylinositol 3-kinase/Akt/mammalian target of rapamycin but not mitogen-activated protein kinase kinase 1/2/extracellular signal-regulated kinase 1/2 cascades. Our findings indicate that an insulin component inducible by glucose is present in the liver of the fish model and may serve as the postprandial signal linking food intake with SPX expression both in the central as well as at the hepatic level.-
dc.languageeng-
dc.publisherOxford University Press. The Journal's web site is located at http://endo.endojournals.org-
dc.relation.ispartofEndocrinology-
dc.rightsThis is a pre-copy-editing, author-produced PDF of an article accepted for publication in Endocrinology following peer review. The definitive publisher-authenticated version Endocrinology, 2017, v. 158 n. 3, p. 560-577 is available online at:https://doi.org/10.1210/en.2016-1534-
dc.titleDual role of insulin in spexin regulation: Functional link between food intake and spexin expression in fish model-
dc.typeArticle-
dc.identifier.emailHe, M: hemu@hkucc.hku.hk-
dc.identifier.emailKo, WKW: wendyko@hku.hk-
dc.identifier.emailWong, AOL: olwong@hkucc.hku.hk-
dc.identifier.authorityWong, AOL=rp00806-
dc.description.naturepostprint-
dc.identifier.doi10.1210/en.2016-1534-
dc.identifier.pmid28359089-
dc.identifier.hkuros282044-
dc.identifier.volume158-
dc.identifier.issue3-
dc.identifier.spage560-
dc.identifier.epage577-
dc.identifier.isiWOS:000397104200015-
dc.publisher.placeUnited States-

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