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Conference Paper: Identification and characterization of a novel receptor for chicken GHRH and the potential receptor(s) for chicken GHRHlike peptide (GHRH-L) and peptide histidine isoleucine (PHI)

TitleIdentification and characterization of a novel receptor for chicken GHRH and the potential receptor(s) for chicken GHRHlike peptide (GHRH-L) and peptide histidine isoleucine (PHI)
Authors
Issue Date2008
PublisherPoultry Science Association Inc. The Meeting Abstracts' web site is located at https://www.poultryscience.org/abstracts.asp
Citation
2008 Poultry Science Association 97th Annual Meeting, Niagara Falls, Ontario, Canada, 20–23 July 2008. In Poultry Science, 2008, v. 87 n. Suppl. 1, p. 37, abstract no. 117 How to Cite?
AbstractGHRH and its structurally related peptides, GHRH-like peptide (GHRH-L, also named PRP), PHI, VIP, and PACAP, are demonstrated to play important roles in the pituitary and extra-pituitary tissues of vertebrates; however, the molecular basis of their actions in various tissues of birds remains largely unknown. In this study, 6 highly homologous receptors for GHRH-L(GHRH-LR), PACAP(PAC1), GHRH(GHRHR1 and GHRHR2), VIP and PHI (VPAC1 and VPAC2), respectively, were cloned from chicken brain (or pituitary) and their functionalities were examined in CHO cells using the pGL3-CRE luciferase reporter system. The results showed that: 1) all receptors are coupled to the cAMP-PKA pathway; 2) 2 GHRH receptors, named GHRHR1 and GHRHR2, respectively, are co-existed in the chicken genome and both of them could be activated by chicken GHRH potently and specifically; 3) Interestingly, 2 VIP receptors, namely VPAC1 and VPAC2, could be potently activated not only by chicken VIP and ovine PACAP38, but also by turkey PHI, suggesting that 2 VIP receptors may also function as the potential receptors specific for PHI in birds; and 4) chicken GHRH-L can activate its receptor, GHRH-LR; however, it also activates the 2 chicken GHRH receptors. Using RT-PCR assay, we further examined the expressions of these 6 receptors in adult chicken tissues. Interestingly, the expressions of GHRH-LR, GHRHR1 and GHRHR2 are mainly restricted to the pituitary and/or different brain regions, while PAC1, VPAC1, and VPAC2 are expressed virtually in all tissues examined. Together, our study not only identified a novel receptor for GHRH and the potential receptor(s) for GHRH-L and PHI in chickens, but also establishes a molecular basis to elucidate the physiological roles of these structurally and functionally related peptides in different tissues, including their actions in the pituitary of chicken.
DescriptionPhysiology, Endocrinology, and Reproduction: Physiology II
Persistent Identifierhttp://hdl.handle.net/10722/257960
ISSN
2019 Impact Factor: 2.659
2015 SCImago Journal Rankings: 1.162

 

DC FieldValueLanguage
dc.contributor.authorWang, Y-
dc.contributor.authorLi, J-
dc.contributor.authorWang, CY-
dc.contributor.authorLeung, FCC-
dc.date.accessioned2018-08-21T02:21:43Z-
dc.date.available2018-08-21T02:21:43Z-
dc.date.issued2008-
dc.identifier.citation2008 Poultry Science Association 97th Annual Meeting, Niagara Falls, Ontario, Canada, 20–23 July 2008. In Poultry Science, 2008, v. 87 n. Suppl. 1, p. 37, abstract no. 117-
dc.identifier.issn0032-5791-
dc.identifier.urihttp://hdl.handle.net/10722/257960-
dc.descriptionPhysiology, Endocrinology, and Reproduction: Physiology II-
dc.description.abstractGHRH and its structurally related peptides, GHRH-like peptide (GHRH-L, also named PRP), PHI, VIP, and PACAP, are demonstrated to play important roles in the pituitary and extra-pituitary tissues of vertebrates; however, the molecular basis of their actions in various tissues of birds remains largely unknown. In this study, 6 highly homologous receptors for GHRH-L(GHRH-LR), PACAP(PAC1), GHRH(GHRHR1 and GHRHR2), VIP and PHI (VPAC1 and VPAC2), respectively, were cloned from chicken brain (or pituitary) and their functionalities were examined in CHO cells using the pGL3-CRE luciferase reporter system. The results showed that: 1) all receptors are coupled to the cAMP-PKA pathway; 2) 2 GHRH receptors, named GHRHR1 and GHRHR2, respectively, are co-existed in the chicken genome and both of them could be activated by chicken GHRH potently and specifically; 3) Interestingly, 2 VIP receptors, namely VPAC1 and VPAC2, could be potently activated not only by chicken VIP and ovine PACAP38, but also by turkey PHI, suggesting that 2 VIP receptors may also function as the potential receptors specific for PHI in birds; and 4) chicken GHRH-L can activate its receptor, GHRH-LR; however, it also activates the 2 chicken GHRH receptors. Using RT-PCR assay, we further examined the expressions of these 6 receptors in adult chicken tissues. Interestingly, the expressions of GHRH-LR, GHRHR1 and GHRHR2 are mainly restricted to the pituitary and/or different brain regions, while PAC1, VPAC1, and VPAC2 are expressed virtually in all tissues examined. Together, our study not only identified a novel receptor for GHRH and the potential receptor(s) for GHRH-L and PHI in chickens, but also establishes a molecular basis to elucidate the physiological roles of these structurally and functionally related peptides in different tissues, including their actions in the pituitary of chicken.-
dc.languageeng-
dc.publisherPoultry Science Association Inc. The Meeting Abstracts' web site is located at https://www.poultryscience.org/abstracts.asp-
dc.relation.ispartofPoultry Science-
dc.relation.ispartofPoultry Science Association Annual Meeting-
dc.titleIdentification and characterization of a novel receptor for chicken GHRH and the potential receptor(s) for chicken GHRHlike peptide (GHRH-L) and peptide histidine isoleucine (PHI)-
dc.typeConference_Paper-
dc.identifier.emailWang, Y: cdwyj@yahoo.com-
dc.identifier.emailLeung, FCC: fcleung@hkucc.hku.hk-
dc.identifier.authorityWang, Y=rp00801-
dc.identifier.authorityLeung, FCC=rp00731-
dc.identifier.hkuros167661-
dc.identifier.volume87-
dc.identifier.issueSuppl. 1-
dc.identifier.spage37-
dc.identifier.epage37-
dc.publisher.placeUnited States-
dc.identifier.issnl0032-5791-

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