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Article: Differentiation of female Oct4‐GFP embryonic stem cells into germ lineage cells

TitleDifferentiation of female Oct4‐GFP embryonic stem cells into germ lineage cells
Authors
KeywordsES
EpiLCs
MEFs
PGCLCs
Cell differentiation
Issue Date2018
PublisherPortland Press Ltd.. The Journal's web site is located at http://www.cellbiolint.org/cbi/default.htm
Citation
Cell Biology International, 2018, v. 42 n. 4, p. 488-494 How to Cite?
AbstractDue to high infertility ratio nowadays, it is essential to explore efficient ways of enhancing mammalian reproductivity, in particular female reproductivity. Using female Oct4‐GFP embryonic stem cells, we mimic the in vivo development procedure to induce ES cells into epiblast cell‐like cells (EpiLCs) and then primordial germ cell‐like cells (PGCLCs). GFP positive PGCLCs that showed typical PGC markers and epigenetic modification were efficiently obtained. Further transplantation of the GFP positive PGCLC and native ovary cell mixture into ovary of infertile mice revealed that both MVH and GFP positive cells could be developed in ovary, but no later developmental stage germ cells were observed. This study suggested that Oct4‐GFP ES cells may be only suitable for tracing early germ cell development.
Persistent Identifierhttp://hdl.handle.net/10722/261883
ISSN
2019 Impact Factor: 2.571
2015 SCImago Journal Rankings: 0.705
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorMa, X-
dc.contributor.authorLi, P-
dc.contributor.authorSun, X-
dc.contributor.authorSun, Y-
dc.contributor.authorHu, R-
dc.contributor.authorYuan, P-
dc.date.accessioned2018-09-28T04:49:45Z-
dc.date.available2018-09-28T04:49:45Z-
dc.date.issued2018-
dc.identifier.citationCell Biology International, 2018, v. 42 n. 4, p. 488-494-
dc.identifier.issn1065-6995-
dc.identifier.urihttp://hdl.handle.net/10722/261883-
dc.description.abstractDue to high infertility ratio nowadays, it is essential to explore efficient ways of enhancing mammalian reproductivity, in particular female reproductivity. Using female Oct4‐GFP embryonic stem cells, we mimic the in vivo development procedure to induce ES cells into epiblast cell‐like cells (EpiLCs) and then primordial germ cell‐like cells (PGCLCs). GFP positive PGCLCs that showed typical PGC markers and epigenetic modification were efficiently obtained. Further transplantation of the GFP positive PGCLC and native ovary cell mixture into ovary of infertile mice revealed that both MVH and GFP positive cells could be developed in ovary, but no later developmental stage germ cells were observed. This study suggested that Oct4‐GFP ES cells may be only suitable for tracing early germ cell development.-
dc.languageeng-
dc.publisherPortland Press Ltd.. The Journal's web site is located at http://www.cellbiolint.org/cbi/default.htm-
dc.relation.ispartofCell Biology International-
dc.rightsThe final version of record is available at [Journal URL].-
dc.subjectES-
dc.subjectEpiLCs-
dc.subjectMEFs-
dc.subjectPGCLCs-
dc.subjectCell differentiation-
dc.titleDifferentiation of female Oct4‐GFP embryonic stem cells into germ lineage cells-
dc.typeArticle-
dc.identifier.emailLi, P: penglihk@hku.hk-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1002/cbin.10918-
dc.identifier.pmid29271529-
dc.identifier.scopuseid_2-s2.0-85040672975-
dc.identifier.hkuros293090-
dc.identifier.volume42-
dc.identifier.issue4-
dc.identifier.spage488-
dc.identifier.epage494-
dc.identifier.isiWOS:000427236900010-
dc.publisher.placeUnited Kingdom-
dc.identifier.issnl1065-6995-

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