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Article: Polymerase-extension-based selection method for DNA-encoded chemical libraries against nonimmobilized protein targets

TitlePolymerase-extension-based selection method for DNA-encoded chemical libraries against nonimmobilized protein targets
Authors
KeywordsDNA-encoded library
drug discovery
high-throughput screening
protein target
polymerase extension
Issue Date2019
PublisherAmerican Chemical Society. The Journal's web site is located at https://pubs.acs.org/journal/acsccc
Citation
ACS Combinatorial Science, 2019, v. 21 n. 5, p. 345-349 How to Cite?
AbstractDNA-encoded chemical libraries (DELs) have become an important ligand discovery technology in biomedical research and drug discovery. DELs can be comprised of hundreds of millions to billions of candidate molecules and provide outstanding chemical diversity for discovering novel ligands and inhibitors for a large variety of biological targets. However, in most cases, DELs are selected against purified and immobilized proteins based on binding affinity. The development and application of DELs to more complex biological targets requires selection methods compatible with nonimmobilized and unpurified proteins. Here, we describe an approach using polymerase-based extension and target-directed photo-cross-linking and its application to the interrogation of a solution-phase protein target, carbonic anhydrase II.
Persistent Identifierhttp://hdl.handle.net/10722/272847
ISSN
2017 Impact Factor: 3.5
2015 SCImago Journal Rankings: 1.037

 

DC FieldValueLanguage
dc.contributor.authorShi, B-
dc.contributor.authorDENG, Y-
dc.contributor.authorLi, X-
dc.date.accessioned2019-08-06T09:17:42Z-
dc.date.available2019-08-06T09:17:42Z-
dc.date.issued2019-
dc.identifier.citationACS Combinatorial Science, 2019, v. 21 n. 5, p. 345-349-
dc.identifier.issn2156-8952-
dc.identifier.urihttp://hdl.handle.net/10722/272847-
dc.description.abstractDNA-encoded chemical libraries (DELs) have become an important ligand discovery technology in biomedical research and drug discovery. DELs can be comprised of hundreds of millions to billions of candidate molecules and provide outstanding chemical diversity for discovering novel ligands and inhibitors for a large variety of biological targets. However, in most cases, DELs are selected against purified and immobilized proteins based on binding affinity. The development and application of DELs to more complex biological targets requires selection methods compatible with nonimmobilized and unpurified proteins. Here, we describe an approach using polymerase-based extension and target-directed photo-cross-linking and its application to the interrogation of a solution-phase protein target, carbonic anhydrase II.-
dc.languageeng-
dc.publisherAmerican Chemical Society. The Journal's web site is located at https://pubs.acs.org/journal/acsccc-
dc.relation.ispartofACS Combinatorial Science-
dc.rightsThis document is the Accepted Manuscript version of a Published Work that appeared in final form in [JournalTitle], copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see [insert ACS Articles on Request author-directed link to Published Work, see http://pubs.acs.org/page/policy/articlesonrequest/index.html].-
dc.subjectDNA-encoded library-
dc.subjectdrug discovery-
dc.subjecthigh-throughput screening-
dc.subjectprotein target-
dc.subjectpolymerase extension-
dc.titlePolymerase-extension-based selection method for DNA-encoded chemical libraries against nonimmobilized protein targets-
dc.typeArticle-
dc.identifier.emailLi, X: xiaoyuli@hku.hk-
dc.identifier.authorityLi, X=rp02080-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1021/acscombsci.9b00011-
dc.identifier.pmid30920794-
dc.identifier.scopuseid_2-s2.0-85064151383-
dc.identifier.hkuros300873-
dc.identifier.volume21-
dc.identifier.issue5-
dc.identifier.spage345-
dc.identifier.epage349-
dc.publisher.placeUnited States-

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