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Article: First case report of fatal Nocardia nova infection in yellow-bibbed lory (Lorius chlorocercus) identified by multilocus sequencing

TitleFirst case report of fatal Nocardia nova infection in yellow-bibbed lory (Lorius chlorocercus) identified by multilocus sequencing
Authors
Issue Date2019
PublisherBioMed Central Ltd.. The Journal's web site is located at http://www.biomedcentral.com/bmcvetres
Citation
BMC Veterinary Research, 2019, v. 15 n. 1, p. article no. 4 How to Cite?
AbstractBackground Nocardiosis is often a multi-systemic disease in humans and other mammals. Nocardiosis in birds is uncommon. Laboratory identification of Nocardia to the species level is difficult by traditional phenotypic methods based on biochemical reactions and hydrolysis tests, and is most accurately performed by sequencing multiple gene targets. Case presentation We report the first case of fatal Nocardia nova infection in a yellow-bibbed lory nestling in an oceanarium diagnosed by multilocus sequencing. Necropsy examination showed effacement of normal sternal musculature with yellowish, firm aberrant material, and diffuse infiltration of the lungs with nodular, tan to yellow foci. Histologically, severe granulomatous inflammation with marked necrosis was observed in the lung, spleen and sternal musculature. Fine, sometimes Gram-positive, 0.5–1 μm wide, branching and beaded filamentous organisms were visible within the lesions. They were acid-fast on Fite-Faraco stain. Tissue samples obtained from the sternum, liver, right lung and right kidney recovered Nocardia species. Sequencing of four gene loci and phylogenetic analysis of concatenated (gyrB-16S-secA1-hsp65) sequences revealed that the isolate was N. nova. Conclusions We report the first case of N. nova infection in yellow-bibbed lorry (Lorius chlorocercus). The present case is the first one of which the species identity of the isolate was determined by multilocus sequencing. Molecular diagnosis is important for identifying the Nocardia to species level and understanding the epidemiology of nocardiosis in birds.
Persistent Identifierhttp://hdl.handle.net/10722/275135
ISSN
2018 Impact Factor: 1.792
2015 SCImago Journal Rankings: 0.952
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorChurgin, SM-
dc.contributor.authorTeng, LL-
dc.contributor.authorHo, JHP-
dc.contributor.authorGraydon, R-
dc.contributor.authorMartelli, P-
dc.contributor.authorLee, FK-
dc.contributor.authorHui, SW-
dc.contributor.authorFong, JYH-
dc.contributor.authorLau, SKP-
dc.contributor.authorWoo, PCY-
dc.date.accessioned2019-09-10T02:36:12Z-
dc.date.available2019-09-10T02:36:12Z-
dc.date.issued2019-
dc.identifier.citationBMC Veterinary Research, 2019, v. 15 n. 1, p. article no. 4-
dc.identifier.issn1746-6148-
dc.identifier.urihttp://hdl.handle.net/10722/275135-
dc.description.abstractBackground Nocardiosis is often a multi-systemic disease in humans and other mammals. Nocardiosis in birds is uncommon. Laboratory identification of Nocardia to the species level is difficult by traditional phenotypic methods based on biochemical reactions and hydrolysis tests, and is most accurately performed by sequencing multiple gene targets. Case presentation We report the first case of fatal Nocardia nova infection in a yellow-bibbed lory nestling in an oceanarium diagnosed by multilocus sequencing. Necropsy examination showed effacement of normal sternal musculature with yellowish, firm aberrant material, and diffuse infiltration of the lungs with nodular, tan to yellow foci. Histologically, severe granulomatous inflammation with marked necrosis was observed in the lung, spleen and sternal musculature. Fine, sometimes Gram-positive, 0.5–1 μm wide, branching and beaded filamentous organisms were visible within the lesions. They were acid-fast on Fite-Faraco stain. Tissue samples obtained from the sternum, liver, right lung and right kidney recovered Nocardia species. Sequencing of four gene loci and phylogenetic analysis of concatenated (gyrB-16S-secA1-hsp65) sequences revealed that the isolate was N. nova. Conclusions We report the first case of N. nova infection in yellow-bibbed lorry (Lorius chlorocercus). The present case is the first one of which the species identity of the isolate was determined by multilocus sequencing. Molecular diagnosis is important for identifying the Nocardia to species level and understanding the epidemiology of nocardiosis in birds.-
dc.languageeng-
dc.publisherBioMed Central Ltd.. The Journal's web site is located at http://www.biomedcentral.com/bmcvetres-
dc.relation.ispartofBMC Veterinary Research-
dc.rightsBMC Veterinary Research. Copyright © BioMed Central Ltd..-
dc.titleFirst case report of fatal Nocardia nova infection in yellow-bibbed lory (Lorius chlorocercus) identified by multilocus sequencing-
dc.typeArticle-
dc.identifier.emailTeng, LL: llteng@hku.hk-
dc.identifier.emailLau, SKP: skplau@hkucc.hku.hk-
dc.identifier.emailWoo, PCY: pcywoo@hkucc.hku.hk-
dc.identifier.authorityTeng, LL=rp00277-
dc.identifier.authorityLau, SKP=rp00486-
dc.identifier.authorityWoo, PCY=rp00430-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1186/s12917-018-1764-x-
dc.identifier.pmid30606196-
dc.identifier.scopuseid_2-s2.0-85059493882-
dc.identifier.hkuros304167-
dc.identifier.volume15-
dc.identifier.issue1-
dc.identifier.spagearticle no. 4-
dc.identifier.epagearticle no. 4-
dc.identifier.isiWOS:000454924100006-
dc.publisher.placeUnited Kingdom-

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