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Article: Antioxidant-mediated protective role of Hericium erinaceus (Bull.: Fr.) Pers. against oxidative damage in fibroblasts from Friedreich's ataxia patient

TitleAntioxidant-mediated protective role of Hericium erinaceus (Bull.: Fr.) Pers. against oxidative damage in fibroblasts from Friedreich's ataxia patient
Authors
KeywordsHericium erinaceus
Friedreich’s ataxia
dermal fibroblasts
antioxidant
oxidative damage
Issue Date2020
PublisherSociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA). The Journal's web site is located at http://www.scielo.br/scielo.php?script=sci_serial&pid=0101-2061&lng=en&nrm=iso
Citation
Food Science and Technology, 2020, v. 40 n. suppl. 1, p. 264-272 How to Cite?
AbstractFriedreich’s ataxia (FRDA) is a progressive neuromuscular disorder caused by substantial decrease of mitochondrial protein frataxin responsible for biogenesis of iron-sulphur clusters and protection from oxidative damage. In this study, we investigated the antioxidant activities of a standardized aqueous extract from fruiting bodies of Hericium erinaceus mushroom (HESAE) and its protective effects against oxidative damage induced by L-Buthionine sulfoximine (BSO) in fibroblasts derived from FRDA patient. The lactate dehydrogenase-based viability assay showed that FRDA fibroblast was sensitive to 12.5 mM BSO with a reduction of viability to 52.51 ± 13.92% after 24 h of BSO exposure. Interestingly, co-incubation with 32 mg/mL HESAE increased the viability to 85.35 ± 3.4%. Further, 12.5 mM BSO caused a decrease in the ratio of cellular reduced glutathione (GSH) to oxidised GSH (GSSG) that leads to cell death. Nevertheless, the damage was reduced by co-incubation with 32 mg/mL HESAE. Nuclear fluorescence staining revealed that 12.5 mM BSO induced cell death and the apoptosis was decreased by co-incubation with HESAE. These findings suggest the ability of HESAE in attenuating BSO-mediated cytotoxicity through maintenance of membrane integrity and optimal GSH/GSSG ratio, that are closely linked to its antioxidant activities. Further in vivo trials are highly warranted to clarify its potential benefits in management of FRDA.
Persistent Identifierhttp://hdl.handle.net/10722/276224
ISSN
2019 Impact Factor: 1.443
2015 SCImago Journal Rankings: 0.340

 

DC FieldValueLanguage
dc.contributor.authorLew, SY-
dc.contributor.authorYow, YY-
dc.contributor.authorLim, LW-
dc.contributor.authorWong, KH-
dc.date.accessioned2019-09-10T02:58:30Z-
dc.date.available2019-09-10T02:58:30Z-
dc.date.issued2020-
dc.identifier.citationFood Science and Technology, 2020, v. 40 n. suppl. 1, p. 264-272-
dc.identifier.issn0101-2061-
dc.identifier.urihttp://hdl.handle.net/10722/276224-
dc.description.abstractFriedreich’s ataxia (FRDA) is a progressive neuromuscular disorder caused by substantial decrease of mitochondrial protein frataxin responsible for biogenesis of iron-sulphur clusters and protection from oxidative damage. In this study, we investigated the antioxidant activities of a standardized aqueous extract from fruiting bodies of Hericium erinaceus mushroom (HESAE) and its protective effects against oxidative damage induced by L-Buthionine sulfoximine (BSO) in fibroblasts derived from FRDA patient. The lactate dehydrogenase-based viability assay showed that FRDA fibroblast was sensitive to 12.5 mM BSO with a reduction of viability to 52.51 ± 13.92% after 24 h of BSO exposure. Interestingly, co-incubation with 32 mg/mL HESAE increased the viability to 85.35 ± 3.4%. Further, 12.5 mM BSO caused a decrease in the ratio of cellular reduced glutathione (GSH) to oxidised GSH (GSSG) that leads to cell death. Nevertheless, the damage was reduced by co-incubation with 32 mg/mL HESAE. Nuclear fluorescence staining revealed that 12.5 mM BSO induced cell death and the apoptosis was decreased by co-incubation with HESAE. These findings suggest the ability of HESAE in attenuating BSO-mediated cytotoxicity through maintenance of membrane integrity and optimal GSH/GSSG ratio, that are closely linked to its antioxidant activities. Further in vivo trials are highly warranted to clarify its potential benefits in management of FRDA.-
dc.languageeng-
dc.publisherSociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA). The Journal's web site is located at http://www.scielo.br/scielo.php?script=sci_serial&pid=0101-2061&lng=en&nrm=iso-
dc.relation.ispartofFood Science and Technology-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.subjectHericium erinaceus-
dc.subjectFriedreich’s ataxia-
dc.subjectdermal fibroblasts-
dc.subjectantioxidant-
dc.subjectoxidative damage-
dc.titleAntioxidant-mediated protective role of Hericium erinaceus (Bull.: Fr.) Pers. against oxidative damage in fibroblasts from Friedreich's ataxia patient-
dc.typeArticle-
dc.identifier.emailLim, LW: limlw@hku.hk-
dc.identifier.authorityLim, LW=rp02088-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1590/fst.09919-
dc.identifier.scopuseid_2-s2.0-85087294565-
dc.identifier.hkuros303554-
dc.identifier.hkuros309289-
dc.identifier.volume40-
dc.identifier.issuesuppl. 1-
dc.identifier.spage264-
dc.identifier.epage272-
dc.publisher.placeBrazil-

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