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Conference Paper: Effect of silver nitrate and sodium fluoride with tri-calcium phosphate on streptococcus mutans and demineralised dentine
Title | Effect of silver nitrate and sodium fluoride with tri-calcium phosphate on streptococcus mutans and demineralised dentine |
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Authors | |
Issue Date | 2018 |
Publisher | International Association for Dental Research. The Journal's web site is located at http://www.iadr.org/ |
Citation | The 32nd International Association for Dental Research (IADR) Southeast Asian Division & 29th South East Asia Association for Dental Education (SEAADE) Annual Scientific Meeting, Da Nang, Vietnam, 11-14 September 2018. In Journal of Dental Research, 2018, v. 97 n. Spec Iss C, article no. 0200 How to Cite? |
Abstract | Objectives: To investigate the adjunctive effect of 25% silver nitrate (AgNO3) solution and 5% sodium fluoride (NaF) varnish with functionalized tri-calcium phosphate (fTCP) on demineralised dentine and cariogenic biofilm.
Methods: 120 human dentine specimens were demineralised and allocated to four groups - Group 1: 25% AgNO3 and 5% NaF + fTCP varnish (Clinpro, 3M); Group 2: 25% AgNO3 (Silver nitrate, Gordon Lab) and 5% NaF (Duraphat, Colgate); Group 3: 25% AgNO3; Group 4: water. After Streptococcus mutans (S. mutans) biofilm challenge for 7 days, the biofilm and dentine surface morphology were studied by scanning electron microscopy (SEM). Lesion depth, crystal characteristics and collagen degradation of specimens were assessed by micro-computed tomography (Micro-CT), X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR), respectively. Confocal laser scanning microscopy (CLSM) and colony forming units (CFU) were used to study the viability of the biofilms.
Results: SEM images revealed severe surface damage in Group 4 and modest surface destruction in Group 3. The mean lesion depths (± standard deviation) from Group 1 to Group 4 were 212.6±20.1µm, 280.8±51.6µm, 402.5±61.7µm and 497.4±67.2µm, respectively (Groups 1<2<3<4, p<0.001). XRD results demonstrated precipitation of silver chloride in Groups 1, 2 and 3. FTIR showed the amide I:HPO42- ratio of the 4 groups were 0.22±0.05, 0.25±0.05, 0.41±0.12 and 0.64±0.14, respectively (Groups 1, 2<3<4; p<0.001). The SEM and CLSM images showed suppression of biofilm growth in Groups 1, 2 and 3. The mean log CFU values of S. mutans (±standard deviation) from Groups 1 to 4 were 4.5±0.7, 4.4±0.9, 4.4±0.9 and 6.7±1.0 (Groups 1,2,3<4, p<0.001).
Conclusions: Addition of NaF with/without fTCP to AgNO3 can reduce the damage of dentine caused by cariogenic biofilm. Besides, the addition does not affect the antibacterial effect of AgNO3. |
Description | Poster Session 7-Cariology and Mineralized Tissues - Presentation ID: 0220 |
Persistent Identifier | http://hdl.handle.net/10722/278678 |
DC Field | Value | Language |
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dc.contributor.author | Yu, OY | - |
dc.contributor.author | Mei, ML | - |
dc.contributor.author | Lo, ECM | - |
dc.contributor.author | Chu, CH | - |
dc.date.accessioned | 2019-10-21T02:11:59Z | - |
dc.date.available | 2019-10-21T02:11:59Z | - |
dc.date.issued | 2018 | - |
dc.identifier.citation | The 32nd International Association for Dental Research (IADR) Southeast Asian Division & 29th South East Asia Association for Dental Education (SEAADE) Annual Scientific Meeting, Da Nang, Vietnam, 11-14 September 2018. In Journal of Dental Research, 2018, v. 97 n. Spec Iss C, article no. 0200 | - |
dc.identifier.uri | http://hdl.handle.net/10722/278678 | - |
dc.description | Poster Session 7-Cariology and Mineralized Tissues - Presentation ID: 0220 | - |
dc.description.abstract | Objectives: To investigate the adjunctive effect of 25% silver nitrate (AgNO3) solution and 5% sodium fluoride (NaF) varnish with functionalized tri-calcium phosphate (fTCP) on demineralised dentine and cariogenic biofilm. Methods: 120 human dentine specimens were demineralised and allocated to four groups - Group 1: 25% AgNO3 and 5% NaF + fTCP varnish (Clinpro, 3M); Group 2: 25% AgNO3 (Silver nitrate, Gordon Lab) and 5% NaF (Duraphat, Colgate); Group 3: 25% AgNO3; Group 4: water. After Streptococcus mutans (S. mutans) biofilm challenge for 7 days, the biofilm and dentine surface morphology were studied by scanning electron microscopy (SEM). Lesion depth, crystal characteristics and collagen degradation of specimens were assessed by micro-computed tomography (Micro-CT), X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR), respectively. Confocal laser scanning microscopy (CLSM) and colony forming units (CFU) were used to study the viability of the biofilms. Results: SEM images revealed severe surface damage in Group 4 and modest surface destruction in Group 3. The mean lesion depths (± standard deviation) from Group 1 to Group 4 were 212.6±20.1µm, 280.8±51.6µm, 402.5±61.7µm and 497.4±67.2µm, respectively (Groups 1<2<3<4, p<0.001). XRD results demonstrated precipitation of silver chloride in Groups 1, 2 and 3. FTIR showed the amide I:HPO42- ratio of the 4 groups were 0.22±0.05, 0.25±0.05, 0.41±0.12 and 0.64±0.14, respectively (Groups 1, 2<3<4; p<0.001). The SEM and CLSM images showed suppression of biofilm growth in Groups 1, 2 and 3. The mean log CFU values of S. mutans (±standard deviation) from Groups 1 to 4 were 4.5±0.7, 4.4±0.9, 4.4±0.9 and 6.7±1.0 (Groups 1,2,3<4, p<0.001). Conclusions: Addition of NaF with/without fTCP to AgNO3 can reduce the damage of dentine caused by cariogenic biofilm. Besides, the addition does not affect the antibacterial effect of AgNO3. | - |
dc.language | eng | - |
dc.publisher | International Association for Dental Research. The Journal's web site is located at http://www.iadr.org/ | - |
dc.relation.ispartof | Journal of Dental Research (Spec Issue) | - |
dc.relation.ispartof | IADR-SEA (International Association for Dental Research (Southeast Asian Division)) Annual Scientific Meeting, Da Nang, Vietnam, 2018 | - |
dc.title | Effect of silver nitrate and sodium fluoride with tri-calcium phosphate on streptococcus mutans and demineralised dentine | - |
dc.type | Conference_Paper | - |
dc.identifier.email | Lo, ECM: edward-lo@hku.hk | - |
dc.identifier.email | Chu, CH: chchu@hku.hk | - |
dc.identifier.authority | Lo, ECM=rp00015 | - |
dc.identifier.authority | Chu, CH=rp00022 | - |
dc.identifier.hkuros | 307272 | - |
dc.identifier.hkuros | 308019 | - |
dc.identifier.volume | 97 | - |
dc.identifier.issue | Spec Iss C | - |
dc.publisher.place | United States | - |