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Article: Spatial Distribution of Biomaterial Microenvironment pH and Its Modulatory Effect on Osteoclasts at the Early Stage of Bone Defect Regeneration

TitleSpatial Distribution of Biomaterial Microenvironment pH and Its Modulatory Effect on Osteoclasts at the Early Stage of Bone Defect Regeneration
Authors
Keywordsmicroenvironment boundary
interfacial pH
osteoclast
biodegradable material
bone regeneration
Issue Date2019
PublisherAmerican Chemical Society. The Journal's web site is located at http://pubs.acs.org/journal/aamick
Citation
ACS Applied Materials & Interfaces, 2019, v. 11 n. 9, p. 9557-9572 How to Cite?
AbstractIt is generally accepted that biodegradable materials greatly influence the nearby microenvironment where cells reside; however, the range of interfacial properties has seldom been discussed due to technical bottlenecks. This study aims to depict biomaterial microenvironment boundaries by correlating interfacial H+ distribution with surrounding cell behaviors. Using a disuse-related osteoporotic mouse model, we confirmed that the abnormal activated osteoclasts could be suppressed under relatively alkaline conditions. The differentiation and apatite-resorption capability of osteoclasts were “switched off” when cultured in titrated material extracts with pH values higher than 7.8. To generate a localized alkaline microenvironment, a series of borosilicates were fabricated and their interfacial H+ distributions were monitored spatiotemporally by employing noninvasive microtest technology. By correlating interfacial H+ distribution with osteoclast “switch on/off” behavior, the microenvironment boundary of the tested material was found to be 400 ± 50 μm, which is broader than the generally accepted value, 300 μm. Furthermore, osteoporotic mice implanted with materials with higher interfacial pH values and boarder effective ranges had lower osteoclast activities and a thicker new bone. To conclude, effective proton microenvironment boundaries of degradable biomaterials were depicted and a weak alkaline microenvironment was shown to promote regeneration of osteoporotic bones possibly by suppressing abnormal activated osteoclasts.
Persistent Identifierhttp://hdl.handle.net/10722/278940
ISSN
2019 Impact Factor: 8.758
2015 SCImago Journal Rankings: 2.381

 

DC FieldValueLanguage
dc.contributor.authorLIU, W-
dc.contributor.authorDAN, X-
dc.contributor.authorLu, WW-
dc.contributor.authorZHAO, X-
dc.contributor.authorRUAN, C-
dc.contributor.authorWANG, T-
dc.contributor.authorCUI, X-
dc.contributor.authorZHAI, X-
dc.contributor.authorMA, Y-
dc.contributor.authorWANG, DP-
dc.contributor.authorHUANG, W-
dc.contributor.authorPAN, H-
dc.date.accessioned2019-10-21T02:16:44Z-
dc.date.available2019-10-21T02:16:44Z-
dc.date.issued2019-
dc.identifier.citationACS Applied Materials & Interfaces, 2019, v. 11 n. 9, p. 9557-9572-
dc.identifier.issn1944-8244-
dc.identifier.urihttp://hdl.handle.net/10722/278940-
dc.description.abstractIt is generally accepted that biodegradable materials greatly influence the nearby microenvironment where cells reside; however, the range of interfacial properties has seldom been discussed due to technical bottlenecks. This study aims to depict biomaterial microenvironment boundaries by correlating interfacial H+ distribution with surrounding cell behaviors. Using a disuse-related osteoporotic mouse model, we confirmed that the abnormal activated osteoclasts could be suppressed under relatively alkaline conditions. The differentiation and apatite-resorption capability of osteoclasts were “switched off” when cultured in titrated material extracts with pH values higher than 7.8. To generate a localized alkaline microenvironment, a series of borosilicates were fabricated and their interfacial H+ distributions were monitored spatiotemporally by employing noninvasive microtest technology. By correlating interfacial H+ distribution with osteoclast “switch on/off” behavior, the microenvironment boundary of the tested material was found to be 400 ± 50 μm, which is broader than the generally accepted value, 300 μm. Furthermore, osteoporotic mice implanted with materials with higher interfacial pH values and boarder effective ranges had lower osteoclast activities and a thicker new bone. To conclude, effective proton microenvironment boundaries of degradable biomaterials were depicted and a weak alkaline microenvironment was shown to promote regeneration of osteoporotic bones possibly by suppressing abnormal activated osteoclasts.-
dc.languageeng-
dc.publisherAmerican Chemical Society. The Journal's web site is located at http://pubs.acs.org/journal/aamick-
dc.relation.ispartofACS Applied Materials & Interfaces-
dc.rightsThis document is the Accepted Manuscript version of a Published Work that appeared in final form in [JournalTitle], copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see [insert ACS Articles on Request author-directed link to Published Work, see http://pubs.acs.org/page/policy/articlesonrequest/index.html].-
dc.subjectmicroenvironment boundary-
dc.subjectinterfacial pH-
dc.subjectosteoclast-
dc.subjectbiodegradable material-
dc.subjectbone regeneration-
dc.titleSpatial Distribution of Biomaterial Microenvironment pH and Its Modulatory Effect on Osteoclasts at the Early Stage of Bone Defect Regeneration-
dc.typeArticle-
dc.identifier.emailLu, WW: wwlu@hku.hk-
dc.identifier.authorityLu, WW=rp00411-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1021/acsami.8b20580-
dc.identifier.pmid30720276-
dc.identifier.scopuseid_2-s2.0-85062370277-
dc.identifier.hkuros307539-
dc.identifier.volume11-
dc.identifier.issue9-
dc.identifier.spage9557-
dc.identifier.epage9572-
dc.publisher.placeUnited States-

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