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Article: Proteomic Analysis of FNR-Regulated Anaerobiosis in Salmonella Typhimurium

TitleProteomic Analysis of FNR-Regulated Anaerobiosis in Salmonella Typhimurium
Authors
KeywordsSalmonella Typhimurium
The FNR regulon
Anaerobiosis
Quantitative proteomics
Issue Date2019
PublisherSpringer New York LLC. The Journal's web site is located at http://www.springer.com/chemistry/analytical+chemistry/journal/13361
Citation
Journal of the American Society for Mass Spectrometry, 2019, v. 30 n. 6, p. 1001-1012 How to Cite?
AbstractBacterial pathogens such as Salmonella enterica serovar Typhimurium (S. Typhimurium) have to cope with fluctuating oxygen levels during infection within host gastrointestinal tracts. The global transcription factor FNR (fumarate nitrate reduction) plays a vital role in the adaptation of enteric bacteria to the low oxygen environment. Nevertheless, a comprehensive profile of the FNR regulon on the proteome level is still lacking in S. Typhimurium. Herein, we quantitatively profiled S. Typhimurium proteome of an fnr-deletion mutant during anaerobiosis in comparison to its parental strain. Notably, we found that FNR represses the expression of virulence genes of Salmonella pathogenicity island 1 (SPI-1) and negatively regulates propanediol utilization by directly binding to the promoter region of the pdu operon. Importantly, we provided evidence that S. Typhimurium lacking fnr exhibited increased antibiotics susceptibility and membrane permeability as well. Furthermore, genetic deletion of fnr leads to decreased bacterial survival in a Caenorhabditis elegans infection model, highlighting an important role of this regulator in mediating host-pathogen interactions.
Persistent Identifierhttp://hdl.handle.net/10722/280235
ISSN
2019 Impact Factor: 3.255
2015 SCImago Journal Rankings: 1.322
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorWang, Z-
dc.contributor.authorSun, J-
dc.contributor.authorTIAN, M-
dc.contributor.authorXu, Z-
dc.contributor.authorLiu, Y-
dc.contributor.authorFu, J-
dc.contributor.authorYan, A-
dc.contributor.authorLiu, X-
dc.date.accessioned2020-01-21T11:50:36Z-
dc.date.available2020-01-21T11:50:36Z-
dc.date.issued2019-
dc.identifier.citationJournal of the American Society for Mass Spectrometry, 2019, v. 30 n. 6, p. 1001-1012-
dc.identifier.issn1044-0305-
dc.identifier.urihttp://hdl.handle.net/10722/280235-
dc.description.abstractBacterial pathogens such as Salmonella enterica serovar Typhimurium (S. Typhimurium) have to cope with fluctuating oxygen levels during infection within host gastrointestinal tracts. The global transcription factor FNR (fumarate nitrate reduction) plays a vital role in the adaptation of enteric bacteria to the low oxygen environment. Nevertheless, a comprehensive profile of the FNR regulon on the proteome level is still lacking in S. Typhimurium. Herein, we quantitatively profiled S. Typhimurium proteome of an fnr-deletion mutant during anaerobiosis in comparison to its parental strain. Notably, we found that FNR represses the expression of virulence genes of Salmonella pathogenicity island 1 (SPI-1) and negatively regulates propanediol utilization by directly binding to the promoter region of the pdu operon. Importantly, we provided evidence that S. Typhimurium lacking fnr exhibited increased antibiotics susceptibility and membrane permeability as well. Furthermore, genetic deletion of fnr leads to decreased bacterial survival in a Caenorhabditis elegans infection model, highlighting an important role of this regulator in mediating host-pathogen interactions.-
dc.languageeng-
dc.publisherSpringer New York LLC. The Journal's web site is located at http://www.springer.com/chemistry/analytical+chemistry/journal/13361-
dc.relation.ispartofJournal of the American Society for Mass Spectrometry-
dc.rightsThis is a post-peer-review, pre-copyedit version of an article published in [insert journal title]. The final authenticated version is available online at: http://dx.doi.org/[insert DOI]-
dc.subjectSalmonella Typhimurium-
dc.subjectThe FNR regulon-
dc.subjectAnaerobiosis-
dc.subjectQuantitative proteomics-
dc.titleProteomic Analysis of FNR-Regulated Anaerobiosis in Salmonella Typhimurium-
dc.typeArticle-
dc.identifier.emailSun, J: jjsun001@hku.hk-
dc.identifier.emailXu, Z: zelingxu@connect.hku.hk-
dc.identifier.emailYan, A: ayan8@hku.hk-
dc.identifier.authorityYan, A=rp00823-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1007/s13361-019-02145-2-
dc.identifier.pmid30903387-
dc.identifier.scopuseid_2-s2.0-85066163674-
dc.identifier.hkuros308987-
dc.identifier.volume30-
dc.identifier.issue6-
dc.identifier.spage1001-
dc.identifier.epage1012-
dc.identifier.isiWOS:000468188500011-
dc.publisher.placeUnited States-
dc.identifier.issnl1044-0305-

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