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Article: Comparative analysis of small RNAs released by the filarial nematode Litomosoides sigmodontis in vitro and in vivo

TitleComparative analysis of small RNAs released by the filarial nematode Litomosoides sigmodontis in vitro and in vivo
Authors
KeywordsMicroRNAs
Parasitic diseases
Transfer RNA
Nematode infections
Macrophages
Issue Date2019
PublisherPublic Library of Science. The Journal's web site is located at http://www.plosntds.org
Citation
PLoS Neglected Tropical Diseases, 2019, v. 13 n. 11, p. article no. e0007811 How to Cite?
AbstractBackground: The release of small non-coding RNAs (sRNAs) has been reported in parasitic nematodes, trematodes and cestodes of medical and veterinary importance. However, little is known regarding the diversity and composition of sRNAs released by different lifecycle stages and the portion of sRNAs that persist in host tissues during filarial infection. This information is relevant to understanding potential roles of sRNAs in parasite-to-host communication, as well as to inform on the location within the host and time point at which they can be detected. Methodology and principal findings: We have used small RNA (sRNA) sequencing analysis to identify sRNAs in replicate samples of the excretory-secretory (ES) products of developmental stages of the filarial nematode Litomosoides sigmodontis in vitro and compare this to the parasite-derived sRNA detected in host tissues. We show that all L. sigmodontis developmental stages release RNAs in vitro, including ribosomal RNA fragments, 5’-derived tRNA fragments (5’-tRFs) and, to a lesser extent, microRNAs (miRNAs). The gravid adult females (gAF) produce the largest diversity and abundance of miRNAs in the ES compared to the adult males or microfilariae. Analysis of sRNAs detected in serum and macrophages from infected animals reveals that parasite miRNAs are preferentially detected in vivo, compared to their low levels in the ES products, and identifies miR-92-3p and miR-71-5p as L. sigmodontis miRNAs that are stably detected in host cells in vivo. Conclusions: Our results suggest that gravid adult female worms secrete the largest diversity of extracellular sRNAs compared to adult males or microfilariae. We further show differences in the parasite sRNA biotype distribution detected in vitro versus in vivo. We identify macrophages as one reservoir for parasite sRNA during infection, and confirm the presence of parasite miRNAs and tRNAs in host serum during patent infection.
Descriptioneid_2-s2.0-85076238339
Persistent Identifierhttp://hdl.handle.net/10722/280266
ISSN
2011 Impact Factor: 4.716
2015 SCImago Journal Rankings: 2.362
PubMed Central ID

 

DC FieldValueLanguage
dc.contributor.authorQuintana, JF-
dc.contributor.authorKumar, S-
dc.contributor.authorIvens, A-
dc.contributor.authorChow, WNF-
dc.contributor.authorHoy, AM-
dc.contributor.authorFulton, A-
dc.contributor.authorDickinson, P-
dc.contributor.authorMartin, C-
dc.contributor.authorTaylor, M-
dc.contributor.authorBabayan, SA-
dc.contributor.authorBuck, AH-
dc.contributor.authorBennuru, S-
dc.date.accessioned2020-01-21T11:50:57Z-
dc.date.available2020-01-21T11:50:57Z-
dc.date.issued2019-
dc.identifier.citationPLoS Neglected Tropical Diseases, 2019, v. 13 n. 11, p. article no. e0007811-
dc.identifier.issn1935-2727-
dc.identifier.urihttp://hdl.handle.net/10722/280266-
dc.descriptioneid_2-s2.0-85076238339-
dc.description.abstractBackground: The release of small non-coding RNAs (sRNAs) has been reported in parasitic nematodes, trematodes and cestodes of medical and veterinary importance. However, little is known regarding the diversity and composition of sRNAs released by different lifecycle stages and the portion of sRNAs that persist in host tissues during filarial infection. This information is relevant to understanding potential roles of sRNAs in parasite-to-host communication, as well as to inform on the location within the host and time point at which they can be detected. Methodology and principal findings: We have used small RNA (sRNA) sequencing analysis to identify sRNAs in replicate samples of the excretory-secretory (ES) products of developmental stages of the filarial nematode Litomosoides sigmodontis in vitro and compare this to the parasite-derived sRNA detected in host tissues. We show that all L. sigmodontis developmental stages release RNAs in vitro, including ribosomal RNA fragments, 5’-derived tRNA fragments (5’-tRFs) and, to a lesser extent, microRNAs (miRNAs). The gravid adult females (gAF) produce the largest diversity and abundance of miRNAs in the ES compared to the adult males or microfilariae. Analysis of sRNAs detected in serum and macrophages from infected animals reveals that parasite miRNAs are preferentially detected in vivo, compared to their low levels in the ES products, and identifies miR-92-3p and miR-71-5p as L. sigmodontis miRNAs that are stably detected in host cells in vivo. Conclusions: Our results suggest that gravid adult female worms secrete the largest diversity of extracellular sRNAs compared to adult males or microfilariae. We further show differences in the parasite sRNA biotype distribution detected in vitro versus in vivo. We identify macrophages as one reservoir for parasite sRNA during infection, and confirm the presence of parasite miRNAs and tRNAs in host serum during patent infection.-
dc.languageeng-
dc.publisherPublic Library of Science. The Journal's web site is located at http://www.plosntds.org-
dc.relation.ispartofPLoS Neglected Tropical Diseases-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.subjectMicroRNAs-
dc.subjectParasitic diseases-
dc.subjectTransfer RNA-
dc.subjectNematode infections-
dc.subjectMacrophages-
dc.titleComparative analysis of small RNAs released by the filarial nematode Litomosoides sigmodontis in vitro and in vivo-
dc.typeArticle-
dc.identifier.emailChow, WNF: chow5810@hku.hk-
dc.identifier.authorityChow, WNF=rp02493-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1371/journal.pntd.0007811-
dc.identifier.pmid31770367-
dc.identifier.pmcidPMC6903752-
dc.identifier.hkuros308934-
dc.identifier.volume13-
dc.identifier.issue11-
dc.identifier.spagearticle no. e0007811-
dc.identifier.epagearticle no. e0007811-
dc.publisher.placeUnited States-

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