File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: PB2 substitutions V598T/I increase the virulence of H7N9 influenza A virus in mammals

TitlePB2 substitutions V598T/I increase the virulence of H7N9 influenza A virus in mammals
Authors
KeywordsPB2
Influenza A virus
Virulence
PB2-V598T/I
PB2-K389R
Issue Date2017
Citation
Virology, 2017, v. 501, p. 92-101 How to Cite?
Abstract© 2016 Elsevier Inc. PB2 is one of the subunits of the influenza A virus (IAV) polymerase complex. By bioinformatics analysis we identified PB2 substitutions at positions 389 and 598 among IAV isolates from humans, which might associate with viral pathogenicity. To evaluate the biological significance of these substitutions, PB2-K389R and -V598T/I mutant viruses of avian H7N9 IAVs were generated by reverse genetics. Compared to the wild type, the mutant viruses displayed an enhanced growth capacity in human and mammalian cells. Meanwhile, they presented increased transcription and replication by producing higher levels of viral mRNA, cRNA and vRNA. Minireplicon assays indicated that the polymerase activity was elevated by these substitutions. Notably, the PB2-V598T/I substitutions substantially increased virus replication and virulence in mice. Together, we demonstrated that the substitutions PB2-V598T/I contributed to higher IAV replication and virulence in mammals, which added to the knowledge of IAV virulence determinants and benefited the surveillance of IAVs.
Persistent Identifierhttp://hdl.handle.net/10722/280609
ISSN
2019 Impact Factor: 2.819
2015 SCImago Journal Rankings: 1.805
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorHu, Meng-
dc.contributor.authorYuan, Shuofeng-
dc.contributor.authorZhang, Ke-
dc.contributor.authorSingh, Kailash-
dc.contributor.authorMa, Qiang-
dc.contributor.authorZhou, Jie-
dc.contributor.authorChu, Hin-
dc.contributor.authorZheng, Bo Jian-
dc.date.accessioned2020-02-17T14:34:28Z-
dc.date.available2020-02-17T14:34:28Z-
dc.date.issued2017-
dc.identifier.citationVirology, 2017, v. 501, p. 92-101-
dc.identifier.issn0042-6822-
dc.identifier.urihttp://hdl.handle.net/10722/280609-
dc.description.abstract© 2016 Elsevier Inc. PB2 is one of the subunits of the influenza A virus (IAV) polymerase complex. By bioinformatics analysis we identified PB2 substitutions at positions 389 and 598 among IAV isolates from humans, which might associate with viral pathogenicity. To evaluate the biological significance of these substitutions, PB2-K389R and -V598T/I mutant viruses of avian H7N9 IAVs were generated by reverse genetics. Compared to the wild type, the mutant viruses displayed an enhanced growth capacity in human and mammalian cells. Meanwhile, they presented increased transcription and replication by producing higher levels of viral mRNA, cRNA and vRNA. Minireplicon assays indicated that the polymerase activity was elevated by these substitutions. Notably, the PB2-V598T/I substitutions substantially increased virus replication and virulence in mice. Together, we demonstrated that the substitutions PB2-V598T/I contributed to higher IAV replication and virulence in mammals, which added to the knowledge of IAV virulence determinants and benefited the surveillance of IAVs.-
dc.languageeng-
dc.relation.ispartofVirology-
dc.subjectPB2-
dc.subjectInfluenza A virus-
dc.subjectVirulence-
dc.subjectPB2-V598T/I-
dc.subjectPB2-K389R-
dc.titlePB2 substitutions V598T/I increase the virulence of H7N9 influenza A virus in mammals-
dc.typeArticle-
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1016/j.virol.2016.11.008-
dc.identifier.pmid27889648-
dc.identifier.scopuseid_2-s2.0-84997785117-
dc.identifier.volume501-
dc.identifier.spage92-
dc.identifier.epage101-
dc.identifier.eissn1096-0341-
dc.identifier.isiWOS:000392263000011-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats