File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Conference Paper: Transcriptomic changes in the nasal epithelium associated with diesel engine exhaust exposure

TitleTranscriptomic changes in the nasal epithelium associated with diesel engine exhaust exposure
Authors
Issue Date2018
PublisherAmerican Thoracic Society. The Journal's web site is located at http://ajrccm.atsjournals.org
Citation
International Conference of the American-Thoracic-Society, San Diego, CA, 18-23 May 2018. In American Journal of Respiratory and Critical Care Medicine, 2018, v. 197, p. abstract no. A7549 How to Cite?
AbstractRATIONALE: Epidemiological studies,show that chronic diesel engine exhaust (DEE) exposure in oocupational settings is associated with lung cancer and other airway diseases. However, the molecular mechanisms by whiich this occurs is not well understood. The goal of this study was to assess transcriptomic alterations in nasal epithelium of DEE exposed factory workers to better understand the cellular and molecular effects of DEE. METHODS: Nasal epithelial brushings were obtained from 41 diesel engine factory workers with known DEE exposure, as well as 38 control subjects who work in factories without DEE exposure. Total RNA was isolated from these samples, and profiled for gene expression using Affymetrix microarrays. Linear modeling was used to identify genes differentially expressed between DEE exposed and control subjects, as well as identifying interaction effects between DEE and active smoking status. Pathway enrichment in differentially expressed genes was assessed using EnrichR. Gene Set Enrichment Analysis (GSEA) was used to compare gene expression patterns across datasets. RESULTS: We found 225 genes whose expression is associated with DEE exposure at FDR q < 0.25, after adjusting for smoking status. These genes were enriched in pathways related to oxidative stress response, cell cycle, and protein modification, as well as transmembrane transport genes, such as CFTR. We further demonstrated that genes up-regulated in the DEE signature are enriched amongst the genes most upregulated by smoking in a previously published dataset. CONCLUSIONS: We characterized the gene expression alterations, resulting from occupational diesel engine exhaust exposure, in the nasal epithelium, including identifying the altered expression of genes involved in xenobiotic metabolism and oxidative stress response. The transcriptomic alterations we identified in the nasal airway may provide insight into airwaydisease pathogenesis and determine differences as well as commonalities in the response of airway epithelium to different inhaled toxins. This abstract is funded by: National Cancer Institute
DescriptionD106 Cellular and Molecular Mechanisms of Environmental and Occupational Exposures - Poster Discussion Session
Persistent Identifierhttp://hdl.handle.net/10722/281187
ISSN
2019 Impact Factor: 17.452
2015 SCImago Journal Rankings: 5.832
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorDrizik, E-
dc.contributor.authorCorbett, S-
dc.contributor.authorVermeulen, R-
dc.contributor.authorDai, Y-
dc.contributor.authorHu, W-
dc.contributor.authorRen, D-
dc.contributor.authorDuan, H-
dc.contributor.authorNiu, Y-
dc.contributor.authorXu, J-
dc.contributor.authorFu, W-
dc.contributor.authorMeliefste, K-
dc.contributor.authorZhou, B-
dc.contributor.authorYang, J-
dc.contributor.authorYe, M-
dc.contributor.authorJia, X-
dc.contributor.authorMeng, T-
dc.contributor.authorBin, P-
dc.contributor.authorZheng, Y-
dc.contributor.authorSilverman, D-
dc.contributor.authorRothman, N-
dc.contributor.authorSpira, A-
dc.contributor.authorLan, Q-
dc.contributor.authorLenburg, ME-
dc.date.accessioned2020-03-09T09:51:21Z-
dc.date.available2020-03-09T09:51:21Z-
dc.date.issued2018-
dc.identifier.citationInternational Conference of the American-Thoracic-Society, San Diego, CA, 18-23 May 2018. In American Journal of Respiratory and Critical Care Medicine, 2018, v. 197, p. abstract no. A7549-
dc.identifier.issn1073-449X-
dc.identifier.urihttp://hdl.handle.net/10722/281187-
dc.descriptionD106 Cellular and Molecular Mechanisms of Environmental and Occupational Exposures - Poster Discussion Session-
dc.description.abstractRATIONALE: Epidemiological studies,show that chronic diesel engine exhaust (DEE) exposure in oocupational settings is associated with lung cancer and other airway diseases. However, the molecular mechanisms by whiich this occurs is not well understood. The goal of this study was to assess transcriptomic alterations in nasal epithelium of DEE exposed factory workers to better understand the cellular and molecular effects of DEE. METHODS: Nasal epithelial brushings were obtained from 41 diesel engine factory workers with known DEE exposure, as well as 38 control subjects who work in factories without DEE exposure. Total RNA was isolated from these samples, and profiled for gene expression using Affymetrix microarrays. Linear modeling was used to identify genes differentially expressed between DEE exposed and control subjects, as well as identifying interaction effects between DEE and active smoking status. Pathway enrichment in differentially expressed genes was assessed using EnrichR. Gene Set Enrichment Analysis (GSEA) was used to compare gene expression patterns across datasets. RESULTS: We found 225 genes whose expression is associated with DEE exposure at FDR q < 0.25, after adjusting for smoking status. These genes were enriched in pathways related to oxidative stress response, cell cycle, and protein modification, as well as transmembrane transport genes, such as CFTR. We further demonstrated that genes up-regulated in the DEE signature are enriched amongst the genes most upregulated by smoking in a previously published dataset. CONCLUSIONS: We characterized the gene expression alterations, resulting from occupational diesel engine exhaust exposure, in the nasal epithelium, including identifying the altered expression of genes involved in xenobiotic metabolism and oxidative stress response. The transcriptomic alterations we identified in the nasal airway may provide insight into airwaydisease pathogenesis and determine differences as well as commonalities in the response of airway epithelium to different inhaled toxins. This abstract is funded by: National Cancer Institute-
dc.languageeng-
dc.publisherAmerican Thoracic Society. The Journal's web site is located at http://ajrccm.atsjournals.org-
dc.relation.ispartofAmerican Journal of Respiratory and Critical Care Medicine-
dc.titleTranscriptomic changes in the nasal epithelium associated with diesel engine exhaust exposure-
dc.typeConference_Paper-
dc.identifier.emailXu, J: xusunjun@hku.hk-
dc.identifier.hkuros309320-
dc.identifier.volume197-
dc.identifier.spageabstract no. A7549-
dc.identifier.epageabstract no. A7549-
dc.identifier.isiWOS:000449980305295-
dc.publisher.placeUnited States-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats