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- Publisher Website: 10.1002/jrsm.1381
- Scopus: eid_2-s2.0-85074858677
- PMID: 31682084
- WOS: WOS:000495485700001
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Article: Pathway-based meta-analysis for partially paired transcriptomics analysis
Title | Pathway-based meta-analysis for partially paired transcriptomics analysis |
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Authors | |
Keywords | Meta‐analysis Partially overlapping samples Pathway analysis Psoriasis |
Issue Date | 2020 |
Publisher | Wiley-Blackwell Publishing Ltd. The Journal's web site is located at http://onlinelibrary.wiley.com/journal/10.1002/(ISSN)1759-2887 |
Citation | Research Synthesis Methods, 2020, v. 11 n. 1, p. 123-133 How to Cite? |
Abstract | Pathway-based differential expression analysis allows the incorporation of biological domain knowledge into transcriptomics analysis to enhance our understanding of disease mechanisms. To integrate information among multiple studies at the pathway level, pathway-based meta-analysis can be performed. Paired or partially paired samples are common in biomedical research. However, there are currently no existing pathway-based meta-analysis methods appropriate for paired or partially paired study designs. In this study, we developed a pathway-based meta-analysis approach for paired or partially paired samples. Meta-analysis on the transcriptomics profiles were conducted using p-value-based, rank-based, and effect size-based algorithms. The application of our approach was demonstrated using partially paired data from psoriasis transcriptomics studies. Upon combining six transcriptomics studies, genes related to the cell cycle and DNA replication pathways are found to be highly perturbed in psoriatic lesional skin samples. Results were validated externally with independent RNA-Seq data. Comparison with existing pathway meta-analysis methods revealed consistent results, with our method showing higher detection power. This study demonstrated the utility of our newly developed pathway-based meta-analysis that allows the incorporation of partially paired or paired samples. The proposed framework can be applied to omics data including but not limited to transcriptomics data. |
Persistent Identifier | http://hdl.handle.net/10722/281766 |
ISSN | 2021 Impact Factor: 9.308 2020 SCImago Journal Rankings: 3.376 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Fung, WT | - |
dc.contributor.author | Wu, JT | - |
dc.contributor.author | Chan, WMM | - |
dc.contributor.author | Chan, HH | - |
dc.contributor.author | Pang, H | - |
dc.date.accessioned | 2020-03-27T04:22:18Z | - |
dc.date.available | 2020-03-27T04:22:18Z | - |
dc.date.issued | 2020 | - |
dc.identifier.citation | Research Synthesis Methods, 2020, v. 11 n. 1, p. 123-133 | - |
dc.identifier.issn | 1759-2879 | - |
dc.identifier.uri | http://hdl.handle.net/10722/281766 | - |
dc.description.abstract | Pathway-based differential expression analysis allows the incorporation of biological domain knowledge into transcriptomics analysis to enhance our understanding of disease mechanisms. To integrate information among multiple studies at the pathway level, pathway-based meta-analysis can be performed. Paired or partially paired samples are common in biomedical research. However, there are currently no existing pathway-based meta-analysis methods appropriate for paired or partially paired study designs. In this study, we developed a pathway-based meta-analysis approach for paired or partially paired samples. Meta-analysis on the transcriptomics profiles were conducted using p-value-based, rank-based, and effect size-based algorithms. The application of our approach was demonstrated using partially paired data from psoriasis transcriptomics studies. Upon combining six transcriptomics studies, genes related to the cell cycle and DNA replication pathways are found to be highly perturbed in psoriatic lesional skin samples. Results were validated externally with independent RNA-Seq data. Comparison with existing pathway meta-analysis methods revealed consistent results, with our method showing higher detection power. This study demonstrated the utility of our newly developed pathway-based meta-analysis that allows the incorporation of partially paired or paired samples. The proposed framework can be applied to omics data including but not limited to transcriptomics data. | - |
dc.language | eng | - |
dc.publisher | Wiley-Blackwell Publishing Ltd. The Journal's web site is located at http://onlinelibrary.wiley.com/journal/10.1002/(ISSN)1759-2887 | - |
dc.relation.ispartof | Research Synthesis Methods | - |
dc.subject | Meta‐analysis | - |
dc.subject | Partially overlapping samples | - |
dc.subject | Pathway analysis | - |
dc.subject | Psoriasis | - |
dc.title | Pathway-based meta-analysis for partially paired transcriptomics analysis | - |
dc.type | Article | - |
dc.identifier.email | Wu, JT: joewu@hku.hk | - |
dc.identifier.email | Chan, WMM: chanwmm@hku.hk | - |
dc.identifier.email | Chan, HH: hhlchan@hkucc.hku.hk | - |
dc.identifier.email | Pang, H: herbpang@hku.hk | - |
dc.identifier.authority | Wu, JT=rp00517 | - |
dc.identifier.authority | Chan, WMM=rp02394 | - |
dc.identifier.authority | Pang, H=rp01857 | - |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1002/jrsm.1381 | - |
dc.identifier.pmid | 31682084 | - |
dc.identifier.scopus | eid_2-s2.0-85074858677 | - |
dc.identifier.hkuros | 309576 | - |
dc.identifier.volume | 11 | - |
dc.identifier.issue | 1 | - |
dc.identifier.spage | 123 | - |
dc.identifier.epage | 133 | - |
dc.identifier.isi | WOS:000495485700001 | - |
dc.publisher.place | United Kingdom | - |
dc.identifier.issnl | 1759-2879 | - |