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Article: Diversity of Dromedary Camel Coronavirus HKU23 in African Camels Revealed Multiple Recombination Events among Closely Related Betacoronaviruses of the Subgenus Embecovirus

TitleDiversity of Dromedary Camel Coronavirus HKU23 in African Camels Revealed Multiple Recombination Events among Closely Related Betacoronaviruses of the Subgenus Embecovirus
Authors
Keywordsbetacoronaviruses
coronaviruses
dromedary camels
evolution
phylogeny
Issue Date2019
PublisherAmerican Society for Microbiology. The Journal's web site is located at http://jvi.asm.org/
Citation
Journal of Virology, 2019, v. 93, p. article no. e01236-19 How to Cite?
AbstractGenetic recombination has frequently been observed in coronaviruses. Here, we sequenced multiple complete genomes of dromedary camel coronavirus HKU23 (DcCoV-HKU23) from Nigeria, Morocco, and Ethiopia and identified several genomic positions indicative of cross-species virus recombination events among other betacoronaviruses of the subgenus Embecovirus (clade A beta-CoVs). Recombinant fragments of a rabbit coronavirus (RbCoV-HKU14) were identified at the hemagglutinin esterase gene position. Homolog fragments of a rodent CoV were also observed at 8.9-kDa open reading frame 4a at the 3' end of the spike gene. The patterns of recombination differed geographically across the African region, highlighting a mosaic structure of DcCoV-HKU23 genomes circulating in dromedaries. Our results highlighted active recombination of coronaviruses circulating in dromedaries and are also relevant to the emergence and evolution of other betacoronaviruses, including Middle East respiratory syndrome coronavirus (MERS-CoV).IMPORTANCE Genetic recombination is often demonstrated in coronaviruses and can result in host range expansion or alteration in tissue tropism. Here, we showed interspecies events of recombination of an endemic dromedary camel coronavirus, HKU23, with other clade A betacoronaviruses. Our results supported the possibility that the zoonotic pathogen MERS-CoV, which also cocirculates in the same camel species, may have undergone similar recombination events facilitating its emergence or may do so in its future evolution. Copyright © 2019 American Society for Microbiology.
Persistent Identifierhttp://hdl.handle.net/10722/281842
ISSN
2019 Impact Factor: 4.501
2015 SCImago Journal Rankings: 3.347
PubMed Central ID

 

DC FieldValueLanguage
dc.contributor.authorSo, TY-
dc.contributor.authorChu, KW-
dc.contributor.authorMiguel, E-
dc.contributor.authorPerera, RAPM-
dc.contributor.authorOladipo, JO-
dc.contributor.authorFassi-Fihri, O-
dc.contributor.authorAylet, G-
dc.contributor.authorKo, LWR-
dc.contributor.authorZhou, Z-
dc.contributor.authorCheng, MS-
dc.contributor.authorKuranga, SA-
dc.contributor.authorRoger, FL-
dc.contributor.authorChevalier, V-
dc.contributor.authorWebby, RJ-
dc.contributor.authorWoo, PCY-
dc.contributor.authorPoon, LML-
dc.contributor.authorPeiris, JSM-
dc.date.accessioned2020-04-03T07:22:34Z-
dc.date.available2020-04-03T07:22:34Z-
dc.date.issued2019-
dc.identifier.citationJournal of Virology, 2019, v. 93, p. article no. e01236-19-
dc.identifier.issn0022-538X-
dc.identifier.urihttp://hdl.handle.net/10722/281842-
dc.description.abstractGenetic recombination has frequently been observed in coronaviruses. Here, we sequenced multiple complete genomes of dromedary camel coronavirus HKU23 (DcCoV-HKU23) from Nigeria, Morocco, and Ethiopia and identified several genomic positions indicative of cross-species virus recombination events among other betacoronaviruses of the subgenus Embecovirus (clade A beta-CoVs). Recombinant fragments of a rabbit coronavirus (RbCoV-HKU14) were identified at the hemagglutinin esterase gene position. Homolog fragments of a rodent CoV were also observed at 8.9-kDa open reading frame 4a at the 3' end of the spike gene. The patterns of recombination differed geographically across the African region, highlighting a mosaic structure of DcCoV-HKU23 genomes circulating in dromedaries. Our results highlighted active recombination of coronaviruses circulating in dromedaries and are also relevant to the emergence and evolution of other betacoronaviruses, including Middle East respiratory syndrome coronavirus (MERS-CoV).IMPORTANCE Genetic recombination is often demonstrated in coronaviruses and can result in host range expansion or alteration in tissue tropism. Here, we showed interspecies events of recombination of an endemic dromedary camel coronavirus, HKU23, with other clade A betacoronaviruses. Our results supported the possibility that the zoonotic pathogen MERS-CoV, which also cocirculates in the same camel species, may have undergone similar recombination events facilitating its emergence or may do so in its future evolution. Copyright © 2019 American Society for Microbiology.-
dc.languageeng-
dc.publisherAmerican Society for Microbiology. The Journal's web site is located at http://jvi.asm.org/-
dc.relation.ispartofJournal of Virology-
dc.rightsJournal of Virology. Copyright © American Society for Microbiology.-
dc.subjectbetacoronaviruses-
dc.subjectcoronaviruses-
dc.subjectdromedary camels-
dc.subjectevolution-
dc.subjectphylogeny-
dc.titleDiversity of Dromedary Camel Coronavirus HKU23 in African Camels Revealed Multiple Recombination Events among Closely Related Betacoronaviruses of the Subgenus Embecovirus-
dc.typeArticle-
dc.identifier.emailChu, KW: dkwchu@hku.hk-
dc.identifier.emailPerera, RAPM: mahenp@hku.hk-
dc.identifier.emailKo, LWR: koronald@hku.hk-
dc.identifier.emailCheng, MS: samuelms@hku.hk-
dc.identifier.emailWoo, PCY: pcywoo@hkucc.hku.hk-
dc.identifier.emailPoon, LML: llmpoon@hkucc.hku.hk-
dc.identifier.emailPeiris, JSM: malik@hkucc.hku.hk-
dc.identifier.authorityChu, KW=rp02512-
dc.identifier.authorityPerera, RAPM=rp02500-
dc.identifier.authorityWoo, PCY=rp00430-
dc.identifier.authorityPoon, LML=rp00484-
dc.identifier.authorityPeiris, JSM=rp00410-
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1128/JVI.01236-19-
dc.identifier.pmid31534035-
dc.identifier.pmcidPMC6854494-
dc.identifier.scopuseid_2-s2.0-85074962491-
dc.identifier.hkuros309640-
dc.identifier.volume93-
dc.identifier.spagearticle no. e01236-
dc.identifier.epage19-
dc.publisher.placeUnited States-

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