File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Impact on cerebral function in rainbow trout fed with plant based omega-3 long chain polyunsaturated fatty acids enriched with DHA and EPA

TitleImpact on cerebral function in rainbow trout fed with plant based omega-3 long chain polyunsaturated fatty acids enriched with DHA and EPA
Authors
KeywordsRainbow trout
ω-3 long chain polyunsaturated fatty acid
Cerebral function
Feed intake
Issue Date2020
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/fsi
Citation
Fish and Shellfish Immunology, 2020, v. 103, p. 409-420 How to Cite?
AbstractCharacterization and modulation of cerebral function by ω-3 long chain polyunsaturated fatty acids (ω-3 LC-PUFAs), eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) enrichment in plant based-diet were studied in rainbow trout (Oncorhynchus mikyss). We hypothesized that ω-3 LC-PUFAs are involved in the regulation of cerebral function in fish. During nine weeks, we examined the growth performance of rainbow trout for three experimental plant based-diets containing distinct levels of EPA and DHA. Using RT-qPCR, we assessed mRNA genes related to feeding behavior regulated by the central nervous system of humans, rodents and fish. These include markers of neuropeptides, indicators of cellular specification, animal stress, oxidant status, cytokines and genes regulating animal behaviour. ω-3 LC-PUFAs enrichment decreased daily food intake and induced a simultaneous mRNA expression increase in orexigenic transcript npy peptide and a decrease in anorexigen transcript pomcA peptide in the hypothalamus. Overall transcript genes related to proinflammatory cytokines, inflammation, antioxidant status, cortisol pathway, serotoninergic pathways and dopaminergic pathways were down-regulated in the juveniles fed the high ω-3 LC-PUFAs diet. However, the mRNA expression of transcripts related to cell specification were down regulated, namely tmem119 markers of microglial cell in forebrain and midbrain, gfap markers of astrocyte in the midbrain, and rbfox3 markers of neurons in the midbrain and hindbrain in juveniles fed high ω-3 experimental diet. In conclusion, this study revealed that a diet rich in ω-3 LC-PUFAs affected a relatively high proportion of the brain function in juvenile rainbow trout through mechanisms comparable to those characterized previously in mammals.
Persistent Identifierhttp://hdl.handle.net/10722/283005
ISSN
2017 Impact Factor: 3.185
2015 SCImago Journal Rankings: 1.311

 

DC FieldValueLanguage
dc.contributor.authorRoy, J-
dc.contributor.authorLarroquet, L-
dc.contributor.authorSurget, A-
dc.contributor.authorLanuque, A-
dc.contributor.authorSandres, F-
dc.contributor.authorTerrier, F-
dc.contributor.authorCorraze, G-
dc.contributor.authorLee, JCY-
dc.contributor.authorSkiba-Cassy, S-
dc.date.accessioned2020-06-05T06:23:57Z-
dc.date.available2020-06-05T06:23:57Z-
dc.date.issued2020-
dc.identifier.citationFish and Shellfish Immunology, 2020, v. 103, p. 409-420-
dc.identifier.issn1050-4648-
dc.identifier.urihttp://hdl.handle.net/10722/283005-
dc.description.abstractCharacterization and modulation of cerebral function by ω-3 long chain polyunsaturated fatty acids (ω-3 LC-PUFAs), eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) enrichment in plant based-diet were studied in rainbow trout (Oncorhynchus mikyss). We hypothesized that ω-3 LC-PUFAs are involved in the regulation of cerebral function in fish. During nine weeks, we examined the growth performance of rainbow trout for three experimental plant based-diets containing distinct levels of EPA and DHA. Using RT-qPCR, we assessed mRNA genes related to feeding behavior regulated by the central nervous system of humans, rodents and fish. These include markers of neuropeptides, indicators of cellular specification, animal stress, oxidant status, cytokines and genes regulating animal behaviour. ω-3 LC-PUFAs enrichment decreased daily food intake and induced a simultaneous mRNA expression increase in orexigenic transcript npy peptide and a decrease in anorexigen transcript pomcA peptide in the hypothalamus. Overall transcript genes related to proinflammatory cytokines, inflammation, antioxidant status, cortisol pathway, serotoninergic pathways and dopaminergic pathways were down-regulated in the juveniles fed the high ω-3 LC-PUFAs diet. However, the mRNA expression of transcripts related to cell specification were down regulated, namely tmem119 markers of microglial cell in forebrain and midbrain, gfap markers of astrocyte in the midbrain, and rbfox3 markers of neurons in the midbrain and hindbrain in juveniles fed high ω-3 experimental diet. In conclusion, this study revealed that a diet rich in ω-3 LC-PUFAs affected a relatively high proportion of the brain function in juvenile rainbow trout through mechanisms comparable to those characterized previously in mammals.-
dc.languageeng-
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/fsi-
dc.relation.ispartofFish and Shellfish Immunology-
dc.subjectRainbow trout-
dc.subjectω-3 long chain polyunsaturated fatty acid-
dc.subjectCerebral function-
dc.subjectFeed intake-
dc.titleImpact on cerebral function in rainbow trout fed with plant based omega-3 long chain polyunsaturated fatty acids enriched with DHA and EPA-
dc.typeArticle-
dc.identifier.emailLee, JCY: jettylee@hku.hk-
dc.identifier.authorityLee, JCY=rp01511-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.fsi.2020.05.044-
dc.identifier.pmid32473359-
dc.identifier.scopuseid_2-s2.0-85085760425-
dc.identifier.hkuros310248-
dc.identifier.volume103-
dc.identifier.spage409-
dc.identifier.epage420-
dc.publisher.placeUnited Kingdom-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats