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Article: Cryogenic 3D printing of heterogeneous scaffolds with gradient mechanical strengths and spatial delivery of osteogenic peptide/TGF-β1 for osteochondral tissue regeneration

TitleCryogenic 3D printing of heterogeneous scaffolds with gradient mechanical strengths and spatial delivery of osteogenic peptide/TGF-β1 for osteochondral tissue regeneration
Authors
KeywordsBiomechanics
Biomimetics
Cartilage
Cell culture
Cryogenics
Issue Date2020
PublisherInstitute of Physics Publishing Ltd.. The Journal's web site is located at http://www.iop.org/EJ/journal/bf
Citation
Biofabrication, 2020, v. 12, p. article no. 025030 How to Cite?
AbstractDue to the increasing aging population and the high probability of sport injury among young people nowadays, it is of great demand to repair/regenerate diseased/defected osteochondral tissue. Given that osteochondral tissue mainly consists of a subchondral layer and a cartilage layer which are structurally heterogeneous and mechanically distinct, developing a biomimetic bi-phasic scaffold with excellent bonding strength to regenerate osteochondral tissue is highly desirable. Three-dimensional (3D) printing is advantageous in producing scaffolds with customized shape, designed structure/composition gradients and hence can be used to produce heterogeneous scaffolds for osteochondral tissue regeneration. In this study, bi-layered osteochondral scaffolds were developed through cryogenic 3D printing, in which osteogenic peptide/β-tricalcium phosphate/poly(lactic-co-glycolic acid) water-in-oil composite emulsions were printed into hierarchically porous subchondral layer while poly(D,L-lactic acid-co-trimethylene carbonate) water-in-oil emulsions were printed into thermal-responsive cartilage frame on top of the subchondral layer. The cartilage frame was further filled/dispensed with transforming growth factor-β1 loaded collagen I hydrogel to form the cartilage module. Although the continuously constructed osteochondral scaffolds had distinct microscopic morphologies and varied mechanical properties at the subchondral zone and cartilage zone at 37 °C, respectively, the two layers were closely bonded together, showing excellent shear strength and peeling strength. Rat bone marrow derived mesenchymal stem cells (rBMSCs) exhibited high viability and proliferation at both subchondral- and cartilage layer. Moreover, gradient rBMSC osteogenic/chondrogenic differentiation was obtained in the osteochondral scaffolds. This proof-of-concept study provides a facile way to produce integrated osteochondral scaffolds for concurrently directing rBMSC osteogenic/chondrogenic differentiation at different regions.
Persistent Identifierhttp://hdl.handle.net/10722/284618
ISSN
2019 Impact Factor: 8.213
2015 SCImago Journal Rankings: 1.505

 

DC FieldValueLanguage
dc.contributor.authorWang, C-
dc.contributor.authorYue, H-
dc.contributor.authorHuang, W-
dc.contributor.authorLin, X-
dc.contributor.authorXie, X-
dc.contributor.authorHe, Z-
dc.contributor.authorHe, X-
dc.contributor.authorLiu, S-
dc.contributor.authorBai, L-
dc.contributor.authorLu, B-
dc.contributor.authorWei, Y-
dc.contributor.authorWang, M-
dc.date.accessioned2020-08-07T09:00:14Z-
dc.date.available2020-08-07T09:00:14Z-
dc.date.issued2020-
dc.identifier.citationBiofabrication, 2020, v. 12, p. article no. 025030-
dc.identifier.issn1758-5082-
dc.identifier.urihttp://hdl.handle.net/10722/284618-
dc.description.abstractDue to the increasing aging population and the high probability of sport injury among young people nowadays, it is of great demand to repair/regenerate diseased/defected osteochondral tissue. Given that osteochondral tissue mainly consists of a subchondral layer and a cartilage layer which are structurally heterogeneous and mechanically distinct, developing a biomimetic bi-phasic scaffold with excellent bonding strength to regenerate osteochondral tissue is highly desirable. Three-dimensional (3D) printing is advantageous in producing scaffolds with customized shape, designed structure/composition gradients and hence can be used to produce heterogeneous scaffolds for osteochondral tissue regeneration. In this study, bi-layered osteochondral scaffolds were developed through cryogenic 3D printing, in which osteogenic peptide/β-tricalcium phosphate/poly(lactic-co-glycolic acid) water-in-oil composite emulsions were printed into hierarchically porous subchondral layer while poly(D,L-lactic acid-co-trimethylene carbonate) water-in-oil emulsions were printed into thermal-responsive cartilage frame on top of the subchondral layer. The cartilage frame was further filled/dispensed with transforming growth factor-β1 loaded collagen I hydrogel to form the cartilage module. Although the continuously constructed osteochondral scaffolds had distinct microscopic morphologies and varied mechanical properties at the subchondral zone and cartilage zone at 37 °C, respectively, the two layers were closely bonded together, showing excellent shear strength and peeling strength. Rat bone marrow derived mesenchymal stem cells (rBMSCs) exhibited high viability and proliferation at both subchondral- and cartilage layer. Moreover, gradient rBMSC osteogenic/chondrogenic differentiation was obtained in the osteochondral scaffolds. This proof-of-concept study provides a facile way to produce integrated osteochondral scaffolds for concurrently directing rBMSC osteogenic/chondrogenic differentiation at different regions.-
dc.languageeng-
dc.publisherInstitute of Physics Publishing Ltd.. The Journal's web site is located at http://www.iop.org/EJ/journal/bf-
dc.relation.ispartofBiofabrication-
dc.rightsBiofabrication. Copyright © Institute of Physics Publishing Ltd..-
dc.subjectBiomechanics-
dc.subjectBiomimetics-
dc.subjectCartilage-
dc.subjectCell culture-
dc.subjectCryogenics-
dc.titleCryogenic 3D printing of heterogeneous scaffolds with gradient mechanical strengths and spatial delivery of osteogenic peptide/TGF-β1 for osteochondral tissue regeneration-
dc.typeArticle-
dc.identifier.emailYue, H: hbyue@hku.hk-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1088/1758-5090/ab7ab5-
dc.identifier.pmid32106097-
dc.identifier.scopuseid_2-s2.0-85082342184-
dc.identifier.hkuros312294-
dc.identifier.volume12-
dc.identifier.spagearticle no. 025030-
dc.identifier.epagearticle no. 025030-
dc.publisher.placeUnited Kingdom-

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