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Article: Proteomic analysis reveals potential factors associated with enhanced EPS production in Streptococcus thermophilus ASCC 1275

TitleProteomic analysis reveals potential factors associated with enhanced EPS production in Streptococcus thermophilus ASCC 1275
Authors
KeywordsExopolysaccharides
Streptococcus Thermophilus
Lactobacillales
Issue Date2020
PublisherNature Research (part of Springer Nature): Fully open access journals. The Journal's web site is located at http://www.nature.com/srep/index.html
Citation
Scientific Reports, 2020, v. 10 n. 1, p. article no. 807 How to Cite?
AbstractStreptococcus thermophilus ASCC 1275 has two chain length determining genes - epsC and epsD- in its eps gene cluster, and produces two times more EPS in sucrose medium than that in glucose and lactose. Hence, we investigated the influence of sugars (glucose, sucrose and lactose), at log phase (5 h) and stationary phase (10 h), on the global proteomics of S. thermophilus 1275 to understand the differentially expressed proteins (DEPs) during EPS production using isobaric tags for relative and absolute quantitation (iTRAQ)-based proteomic analysis. Among 98 DEPs in sucrose medium, most of them were mapped into EPS biosynthesis pathway and other related metabolisms. There was an upregulation of several proteins involved in sugar transport (phosphoenolpyruvate (PEP) phosphotransferase system), EPS assembly (epsG1D) and amino acid metabolism (methionine, cysteine/arginine metabolism) in sucrose medium. This study showed that increased EPS production in S. thermophilus 1275 requires a well-co-ordinated regulation of pathway involved in both EPS assembly and amino acid metabolism along with the availability of sugars. Thus, it provided valuable insights into the biosynthesis and regulation of EPS in S. thermophilus 1275, and potential gene targets for understanding high-EPS strains.
Persistent Identifierhttp://hdl.handle.net/10722/284879
ISSN
2019 Impact Factor: 3.998
2015 SCImago Journal Rankings: 2.073
PubMed Central ID

 

DC FieldValueLanguage
dc.contributor.authorPadmanabhan, A-
dc.contributor.authorTong, Y-
dc.contributor.authorWu, Q-
dc.contributor.authorLo, C-
dc.contributor.authorShah, NP-
dc.date.accessioned2020-08-07T09:03:49Z-
dc.date.available2020-08-07T09:03:49Z-
dc.date.issued2020-
dc.identifier.citationScientific Reports, 2020, v. 10 n. 1, p. article no. 807-
dc.identifier.issn2045-2322-
dc.identifier.urihttp://hdl.handle.net/10722/284879-
dc.description.abstractStreptococcus thermophilus ASCC 1275 has two chain length determining genes - epsC and epsD- in its eps gene cluster, and produces two times more EPS in sucrose medium than that in glucose and lactose. Hence, we investigated the influence of sugars (glucose, sucrose and lactose), at log phase (5 h) and stationary phase (10 h), on the global proteomics of S. thermophilus 1275 to understand the differentially expressed proteins (DEPs) during EPS production using isobaric tags for relative and absolute quantitation (iTRAQ)-based proteomic analysis. Among 98 DEPs in sucrose medium, most of them were mapped into EPS biosynthesis pathway and other related metabolisms. There was an upregulation of several proteins involved in sugar transport (phosphoenolpyruvate (PEP) phosphotransferase system), EPS assembly (epsG1D) and amino acid metabolism (methionine, cysteine/arginine metabolism) in sucrose medium. This study showed that increased EPS production in S. thermophilus 1275 requires a well-co-ordinated regulation of pathway involved in both EPS assembly and amino acid metabolism along with the availability of sugars. Thus, it provided valuable insights into the biosynthesis and regulation of EPS in S. thermophilus 1275, and potential gene targets for understanding high-EPS strains.-
dc.languageeng-
dc.publisherNature Research (part of Springer Nature): Fully open access journals. The Journal's web site is located at http://www.nature.com/srep/index.html-
dc.relation.ispartofScientific Reports-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.subjectExopolysaccharides-
dc.subjectStreptococcus Thermophilus-
dc.subjectLactobacillales-
dc.titleProteomic analysis reveals potential factors associated with enhanced EPS production in Streptococcus thermophilus ASCC 1275-
dc.typeArticle-
dc.identifier.emailPadmanabhan, A: aparnaid@hku.hk-
dc.identifier.emailTong, Y: tongyin9@hku.hk-
dc.identifier.emailLo, C: clivelo@hku.hk-
dc.identifier.emailShah, NP: npshah@hku.hk-
dc.identifier.authorityLo, C=rp00751-
dc.identifier.authorityShah, NP=rp01571-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1038/s41598-020-57665-9-
dc.identifier.pmid31964939-
dc.identifier.pmcidPMC6972726-
dc.identifier.scopuseid_2-s2.0-85078192253-
dc.identifier.hkuros312369-
dc.identifier.volume10-
dc.identifier.issue1-
dc.identifier.spagearticle no. 807-
dc.identifier.epagearticle no. 807-
dc.publisher.placeUnited Kingdom-

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