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Article: Transmissible Retrovirus in Epstein-Burr Virus-Producer B95-8 Cells

TitleTransmissible Retrovirus in Epstein-Burr Virus-Producer B95-8 Cells
Authors
Issue Date1995
Citation
Virology, 1995, v. 209, n. 2, p. 374-383 How to Cite?
AbstractEpstein-Burr virus (EBV) released from the B95-8 marmoset cell line has served as a prototype for biologic and biochemical studies of EBV. Here we identify and characterize a retrovirus carried by many cultures of B95-8 cells. The experiments were stimulated by the isolation of a cDNA clone from B95-8 cells in which sequences from the EBV large internal repeat were linked to gag sequences similar to those of squirrel monkey retrovirus, human isolate, SMRV-H. However, among 413 amino acids predicted from the nucleotide sequence of the gag region of the B95-8 SMRV isolate there were 48 amino acid changes that distinguished this virus from SMRV-H originally isolated from a human lymphoid cell line by Oda et al. (1988, Virology 167, 468-476). Nucleic acid and antibody probes were developed for the B95-8 isolate of SMRV. Using such probes, we found that SMRV-B95-8 was readily transmissible, independent of EBV, as an infectious virus to human and T cell lines, SMRV-B95-8 was highly fusogenic in the presence or absence of EBV. The ultrastructural appearance of the B95-8 retrovirus was characteristic of a type D retrovirus. Cells dually infected with EBV and SMRV-B95-8 did not demonstrate increased levels of lyric EB vital replication. SMRV-B95-8 did not by itself cause lymphocyte immortalization or enhance immortalization by EBV. Thus SMRV-B95-8 does not contribute to the major biologic properties of the B95-8 strain of EBV. © 1995 Academic Press. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/285551
ISSN
2019 Impact Factor: 2.819
2015 SCImago Journal Rankings: 1.805

 

DC FieldValueLanguage
dc.contributor.authorSun, Ren-
dc.contributor.authorGrogan, Elizabeth-
dc.contributor.authorShedd, Duane-
dc.contributor.authorBykovsky, Albert F.-
dc.contributor.authorKushnaryov, Vladimir M.-
dc.contributor.authorGrossberg, Sidney E.-
dc.contributor.authorMiller, George-
dc.date.accessioned2020-08-18T04:56:02Z-
dc.date.available2020-08-18T04:56:02Z-
dc.date.issued1995-
dc.identifier.citationVirology, 1995, v. 209, n. 2, p. 374-383-
dc.identifier.issn0042-6822-
dc.identifier.urihttp://hdl.handle.net/10722/285551-
dc.description.abstractEpstein-Burr virus (EBV) released from the B95-8 marmoset cell line has served as a prototype for biologic and biochemical studies of EBV. Here we identify and characterize a retrovirus carried by many cultures of B95-8 cells. The experiments were stimulated by the isolation of a cDNA clone from B95-8 cells in which sequences from the EBV large internal repeat were linked to gag sequences similar to those of squirrel monkey retrovirus, human isolate, SMRV-H. However, among 413 amino acids predicted from the nucleotide sequence of the gag region of the B95-8 SMRV isolate there were 48 amino acid changes that distinguished this virus from SMRV-H originally isolated from a human lymphoid cell line by Oda et al. (1988, Virology 167, 468-476). Nucleic acid and antibody probes were developed for the B95-8 isolate of SMRV. Using such probes, we found that SMRV-B95-8 was readily transmissible, independent of EBV, as an infectious virus to human and T cell lines, SMRV-B95-8 was highly fusogenic in the presence or absence of EBV. The ultrastructural appearance of the B95-8 retrovirus was characteristic of a type D retrovirus. Cells dually infected with EBV and SMRV-B95-8 did not demonstrate increased levels of lyric EB vital replication. SMRV-B95-8 did not by itself cause lymphocyte immortalization or enhance immortalization by EBV. Thus SMRV-B95-8 does not contribute to the major biologic properties of the B95-8 strain of EBV. © 1995 Academic Press. All rights reserved.-
dc.languageeng-
dc.relation.ispartofVirology-
dc.titleTransmissible Retrovirus in Epstein-Burr Virus-Producer B95-8 Cells-
dc.typeArticle-
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1006/viro.1995.1269-
dc.identifier.pmid7778272-
dc.identifier.scopuseid_2-s2.0-0029007411-
dc.identifier.volume209-
dc.identifier.issue2-
dc.identifier.spage374-
dc.identifier.epage383-
dc.identifier.eissn1096-0341-

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