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Article: Characterization of Kaposi's sarcoma-associated herpesvirus (KSHV) K1 promoter activation by Rta

TitleCharacterization of Kaposi's sarcoma-associated herpesvirus (KSHV) K1 promoter activation by Rta
Authors
KeywordsHHV8
KSHV
Orf50
Rta
Transcription
K1
Issue Date2006
Citation
Virology, 2006, v. 348, n. 2, p. 309-327 How to Cite?
AbstractThe K1 gene of Kaposi's sarcoma-associated herpesvirus (KSHV) encodes a 46-kDa transmembrane glycoprotein that possesses transforming properties, initiates signaling pathways in B cells, and prevents apoptosis. Here, we demonstrate a mechanism for activation of the K1 promoter by the Rta transactivator. Electrophoretic mobility shift assay (EMSA) analysis of the K1 promoter demonstrated that purified Rta protein bound to the K1 promoter at three locations, independent of other DNA-binding factors. Transcriptional assays revealed that only two of these Rta DNA-binding sites are functionally significant, and that they could impart Rta responsiveness to a heterologous E4 TATA box promoter. In addition, TATA-binding protein (TBP) bound to a TATA box element located 25 bp upstream of the K1 transcription start site and was also shown to associate with Rta by coimmunoprecipitation analysis. Rta transactivation may therefore be mediated in part through recruitment of TBP to target promoters. © 2006 Elsevier Inc. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/285901
ISSN
2019 Impact Factor: 2.819
2015 SCImago Journal Rankings: 1.805

 

DC FieldValueLanguage
dc.contributor.authorBowser, Brian S.-
dc.contributor.authorMorris, Stephanie-
dc.contributor.authorSong, Moon Jung-
dc.contributor.authorSun, Ren-
dc.contributor.authorDamania, Blossom-
dc.date.accessioned2020-08-18T04:56:56Z-
dc.date.available2020-08-18T04:56:56Z-
dc.date.issued2006-
dc.identifier.citationVirology, 2006, v. 348, n. 2, p. 309-327-
dc.identifier.issn0042-6822-
dc.identifier.urihttp://hdl.handle.net/10722/285901-
dc.description.abstractThe K1 gene of Kaposi's sarcoma-associated herpesvirus (KSHV) encodes a 46-kDa transmembrane glycoprotein that possesses transforming properties, initiates signaling pathways in B cells, and prevents apoptosis. Here, we demonstrate a mechanism for activation of the K1 promoter by the Rta transactivator. Electrophoretic mobility shift assay (EMSA) analysis of the K1 promoter demonstrated that purified Rta protein bound to the K1 promoter at three locations, independent of other DNA-binding factors. Transcriptional assays revealed that only two of these Rta DNA-binding sites are functionally significant, and that they could impart Rta responsiveness to a heterologous E4 TATA box promoter. In addition, TATA-binding protein (TBP) bound to a TATA box element located 25 bp upstream of the K1 transcription start site and was also shown to associate with Rta by coimmunoprecipitation analysis. Rta transactivation may therefore be mediated in part through recruitment of TBP to target promoters. © 2006 Elsevier Inc. All rights reserved.-
dc.languageeng-
dc.relation.ispartofVirology-
dc.subjectHHV8-
dc.subjectKSHV-
dc.subjectOrf50-
dc.subjectRta-
dc.subjectTranscription-
dc.subjectK1-
dc.titleCharacterization of Kaposi's sarcoma-associated herpesvirus (KSHV) K1 promoter activation by Rta-
dc.typeArticle-
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1016/j.virol.2006.02.007-
dc.identifier.pmid16546233-
dc.identifier.scopuseid_2-s2.0-33646486990-
dc.identifier.volume348-
dc.identifier.issue2-
dc.identifier.spage309-
dc.identifier.epage327-
dc.identifier.eissn1096-0341-

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