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Article: Dimethylaminododecyl methacrylate inhibits Candida albicans and oropharyngeal candidiasis in a pH-dependent manner

TitleDimethylaminododecyl methacrylate inhibits Candida albicans and oropharyngeal candidiasis in a pH-dependent manner
Authors
KeywordsFungal infection
Biofilm
Quaternary ammoniums
Salivary pH variation
Surface electrical charge
Issue Date2020
PublisherSpringer. The Journal's web site is located at http://link.springer.de/link/service/journals/00253/index.htm
Citation
Applied Microbiology and Biotechnology, 2020, v. 104 n. 8, p. 3585-3595 How to Cite?
AbstractThe prevalence of stomatitis, especially that caused by Candida albicans, has highlighted the need for new antifungal agents. We previously found that a type of quaternary ammonium salts, dimethylaminododecyl methacrylate (DMADDM), incorporated in dental materials inhibited the growth and hyphal development of C. albicans. However, how the quaternary ammonium salts inhibited the fungal pathogens and whether the oral condition, such as salivary pH variation under different diseases, can affect the antimicrobial capacity of quaternary ammonium salts is unknown. This study evaluated the antifungal effects of DMADDM at different pH in vitro and in vivo. A pH-dependent antifungal effect of DMADDM was observed in planktonic and biofilm growth. DMADDM enhanced antifungal activity at alkaline pH. Two pH-regulated genes (PHR1/PHR2) of C. albicans were correlated with the pH-dependent antifungal effects of DMADDM. The PHR1/PHR2 genes and pH values regulated the zeta potential of C. albicans, which then influenced the binding between C. albicans cells and DMADDM. The pH-dependent antifungal activity of DMADDM was then substantiated in a murine oropharyngeal candidiasis model. We directly demonstrated that the antifungal abilities of quaternary ammonium salts relied on the cell zeta potential which affected the binding between fungal cells and quaternary ammonium salts. These findings suggest a new antifungal mechanism of quaternary ammonium under different pH and that DMADDM can be a potential antifungal agent applied in dental materials and stomatitis therapy.
Persistent Identifierhttp://hdl.handle.net/10722/287881
ISSN
2020 Impact Factor: 4.813
2020 SCImago Journal Rankings: 1.074
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorChen, H-
dc.contributor.authorZhou, Y-
dc.contributor.authorZhou, X-
dc.contributor.authorLiao, B-
dc.contributor.authorXu, HHK-
dc.contributor.authorChu, CH-
dc.contributor.authorCheng, L-
dc.contributor.authorRen, B-
dc.date.accessioned2020-10-05T12:04:36Z-
dc.date.available2020-10-05T12:04:36Z-
dc.date.issued2020-
dc.identifier.citationApplied Microbiology and Biotechnology, 2020, v. 104 n. 8, p. 3585-3595-
dc.identifier.issn0175-7598-
dc.identifier.urihttp://hdl.handle.net/10722/287881-
dc.description.abstractThe prevalence of stomatitis, especially that caused by Candida albicans, has highlighted the need for new antifungal agents. We previously found that a type of quaternary ammonium salts, dimethylaminododecyl methacrylate (DMADDM), incorporated in dental materials inhibited the growth and hyphal development of C. albicans. However, how the quaternary ammonium salts inhibited the fungal pathogens and whether the oral condition, such as salivary pH variation under different diseases, can affect the antimicrobial capacity of quaternary ammonium salts is unknown. This study evaluated the antifungal effects of DMADDM at different pH in vitro and in vivo. A pH-dependent antifungal effect of DMADDM was observed in planktonic and biofilm growth. DMADDM enhanced antifungal activity at alkaline pH. Two pH-regulated genes (PHR1/PHR2) of C. albicans were correlated with the pH-dependent antifungal effects of DMADDM. The PHR1/PHR2 genes and pH values regulated the zeta potential of C. albicans, which then influenced the binding between C. albicans cells and DMADDM. The pH-dependent antifungal activity of DMADDM was then substantiated in a murine oropharyngeal candidiasis model. We directly demonstrated that the antifungal abilities of quaternary ammonium salts relied on the cell zeta potential which affected the binding between fungal cells and quaternary ammonium salts. These findings suggest a new antifungal mechanism of quaternary ammonium under different pH and that DMADDM can be a potential antifungal agent applied in dental materials and stomatitis therapy.-
dc.languageeng-
dc.publisherSpringer. The Journal's web site is located at http://link.springer.de/link/service/journals/00253/index.htm-
dc.relation.ispartofApplied Microbiology and Biotechnology-
dc.rightsThis is a post-peer-review, pre-copyedit version of an article published in Applied Microbiology and Biotechnology. The final authenticated version is available online at: https://doi.org/10.1007/s00253-020-10496-0-
dc.subjectFungal infection-
dc.subjectBiofilm-
dc.subjectQuaternary ammoniums-
dc.subjectSalivary pH variation-
dc.subjectSurface electrical charge-
dc.titleDimethylaminododecyl methacrylate inhibits Candida albicans and oropharyngeal candidiasis in a pH-dependent manner-
dc.typeArticle-
dc.identifier.emailChu, CH: chchu@hku.hk-
dc.identifier.authorityChu, CH=rp00022-
dc.description.naturepostprint-
dc.identifier.doi10.1007/s00253-020-10496-0-
dc.identifier.pmid32125481-
dc.identifier.scopuseid_2-s2.0-85081548166-
dc.identifier.hkuros315785-
dc.identifier.volume104-
dc.identifier.issue8-
dc.identifier.spage3585-
dc.identifier.epage3595-
dc.identifier.isiWOS:000518077800007-
dc.publisher.placeGermany-
dc.identifier.issnl0175-7598-

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