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Article: Lycium barbarum Polysaccharide Suppresses Expression of Fibrotic Proteins in Primary Human Corneal Fibroblasts

TitleLycium barbarum Polysaccharide Suppresses Expression of Fibrotic Proteins in Primary Human Corneal Fibroblasts
Authors
KeywordsLycium barbarum polysaccharides
corneal scarring
human keratocytes
fibroblast
TGFβ1
Issue Date2020
PublisherMDPI AG. The Journal's web site is located at http://www.mdpi.com/journal/jcm
Citation
Journal of Clinical Medicine, 2020, v. 9 n. 11, p. article no. 3572 How to Cite?
Abstract(1) Objective: To study the anti-fibrotic effects of Lycium barbarum polysaccharides (LBP) on corneal stromal fibroblasts and assess LBP’s effect on cell viability. (2) Methods: Primary human corneal keratocytes of passage 3 to 6 were used for all experiments. Cells are pretreated with LBP solution for 24 h and then transforming growth factor beta 1 (TGFβ1) for 48 h and collected for experiments. Fibrotic protein analysis was performed using immunofluorescence and Western blot. The effect of LBP on cell viability was assessed using the MTS assay. (3) Results: LBP significantly reduced the expression of fibrotic proteins, including α-SMA and extracellular matrix proteins (collagen type I and III). LBP significantly decreased the viability of myofibroblasts but not the fibroblasts. Conclusions: In this study, LBP was effective in the prevention of fibrosis gene expression. Further studies to assess the underlying mechanism and pharmacological properties will facilitate the formation of a topical LBP solution for in vivo studies.
Persistent Identifierhttp://hdl.handle.net/10722/293488
ISSN
2018 Impact Factor: 5.688
PubMed Central ID
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorKwok, SS-
dc.contributor.authorWong, SY-
dc.contributor.authorShih, KC-
dc.contributor.authorChan, YK-
dc.contributor.authorBU, Y-
dc.contributor.authorChan, TCY-
dc.contributor.authorNg, ALK-
dc.contributor.authorLo, ACY-
dc.contributor.authorTong, L-
dc.contributor.authorYam, GHF-
dc.contributor.authorJhanji, V-
dc.date.accessioned2020-11-23T08:17:30Z-
dc.date.available2020-11-23T08:17:30Z-
dc.date.issued2020-
dc.identifier.citationJournal of Clinical Medicine, 2020, v. 9 n. 11, p. article no. 3572-
dc.identifier.issn2077-0383-
dc.identifier.urihttp://hdl.handle.net/10722/293488-
dc.description.abstract(1) Objective: To study the anti-fibrotic effects of Lycium barbarum polysaccharides (LBP) on corneal stromal fibroblasts and assess LBP’s effect on cell viability. (2) Methods: Primary human corneal keratocytes of passage 3 to 6 were used for all experiments. Cells are pretreated with LBP solution for 24 h and then transforming growth factor beta 1 (TGFβ1) for 48 h and collected for experiments. Fibrotic protein analysis was performed using immunofluorescence and Western blot. The effect of LBP on cell viability was assessed using the MTS assay. (3) Results: LBP significantly reduced the expression of fibrotic proteins, including α-SMA and extracellular matrix proteins (collagen type I and III). LBP significantly decreased the viability of myofibroblasts but not the fibroblasts. Conclusions: In this study, LBP was effective in the prevention of fibrosis gene expression. Further studies to assess the underlying mechanism and pharmacological properties will facilitate the formation of a topical LBP solution for in vivo studies.-
dc.languageeng-
dc.publisherMDPI AG. The Journal's web site is located at http://www.mdpi.com/journal/jcm-
dc.relation.ispartofJournal of Clinical Medicine-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.subjectLycium barbarum polysaccharides-
dc.subjectcorneal scarring-
dc.subjecthuman keratocytes-
dc.subjectfibroblast-
dc.subjectTGFβ1-
dc.titleLycium barbarum Polysaccharide Suppresses Expression of Fibrotic Proteins in Primary Human Corneal Fibroblasts-
dc.typeArticle-
dc.identifier.emailShih, KC: kcshih@hku.hk-
dc.identifier.emailChan, YK: josephyk@connect.hku.hk-
dc.identifier.emailLo, ACY: amylo@hku.hk-
dc.identifier.authorityShih, KC=rp01374-
dc.identifier.authorityChan, YK=rp02536-
dc.identifier.authorityNg, ALK=rp01842-
dc.identifier.authorityLo, ACY=rp00425-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.3390/jcm9113572-
dc.identifier.pmid33171906-
dc.identifier.pmcidPMC7694544-
dc.identifier.hkuros319096-
dc.identifier.volume9-
dc.identifier.issue11-
dc.identifier.spagearticle no. 3572-
dc.identifier.epagearticle no. 3572-
dc.identifier.isiWOS:000593286200001-
dc.publisher.placeSwitzerland-

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