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Article: MinION Nanopore Sequencing Enables Correlation between Resistome Phenotype and Genotype of Coliform Bacteria in Municipal Sewage

TitleMinION Nanopore Sequencing Enables Correlation between Resistome Phenotype and Genotype of Coliform Bacteria in Municipal Sewage
Authors
KeywordsMinION
ARGS
fecal coliforms
sewage
nanopore
Issue Date2017
PublisherFrontiers Research Foundation. The Journal's web site is located at http://www.frontiersin.org/microbiology/
Citation
Frontiers in Microbiology, 2017, v. 8, article no. 2105 How to Cite?
AbstractWastewater treatment plants (WWTPs) functioned as the intersection between the human society and nature environment, are receiving increasingly more attention on risk assessment of the acquisition of environmental antibiotic resistance genes (ARGs) by pathogenetic populations during treatment. However, because of the general lack of robust resistome profiling methods, genotype, and resistance phenotype is still poorly correlated in human pathogens of sewage samples. Here we applied MinION sequencing to quantify the resistance genes of multiple antibiotic resistant (MAR) coliform bacteria, a common indicator for human enteric pathogens in sewage samples. Our pipeline could deliver the results within 30 h from sample collection and the resistome quantification was consistent to that based on the Illumina platform. Additionally, the long nanopore reads not only enabled a simultaneous identification of the carrier populations of ARGs detected, but also facilitated the genome reconstruction of a representative MAR strain, from which we identified an instance of chromosomal integration of environmental resistance gene obtained by plasmid exchange with a porcine pathogen. This study demonstrated the utilization of MinION sequencing in quick monitoring and simultaneous phylogenetic tracking of environmental ARGs to address potential health risk associated with them.
Persistent Identifierhttp://hdl.handle.net/10722/293571
ISSN
2019 Impact Factor: 4.235
2015 SCImago Journal Rankings: 1.970
PubMed Central ID
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorXia, Y-
dc.contributor.authorLi, A-
dc.contributor.authorDeng, Y-
dc.contributor.authorJiang, XT-
dc.contributor.authorLi, LG-
dc.contributor.authorZhang, T-
dc.date.accessioned2020-11-23T08:18:42Z-
dc.date.available2020-11-23T08:18:42Z-
dc.date.issued2017-
dc.identifier.citationFrontiers in Microbiology, 2017, v. 8, article no. 2105-
dc.identifier.issn1664-302X-
dc.identifier.urihttp://hdl.handle.net/10722/293571-
dc.description.abstractWastewater treatment plants (WWTPs) functioned as the intersection between the human society and nature environment, are receiving increasingly more attention on risk assessment of the acquisition of environmental antibiotic resistance genes (ARGs) by pathogenetic populations during treatment. However, because of the general lack of robust resistome profiling methods, genotype, and resistance phenotype is still poorly correlated in human pathogens of sewage samples. Here we applied MinION sequencing to quantify the resistance genes of multiple antibiotic resistant (MAR) coliform bacteria, a common indicator for human enteric pathogens in sewage samples. Our pipeline could deliver the results within 30 h from sample collection and the resistome quantification was consistent to that based on the Illumina platform. Additionally, the long nanopore reads not only enabled a simultaneous identification of the carrier populations of ARGs detected, but also facilitated the genome reconstruction of a representative MAR strain, from which we identified an instance of chromosomal integration of environmental resistance gene obtained by plasmid exchange with a porcine pathogen. This study demonstrated the utilization of MinION sequencing in quick monitoring and simultaneous phylogenetic tracking of environmental ARGs to address potential health risk associated with them.-
dc.languageeng-
dc.publisherFrontiers Research Foundation. The Journal's web site is located at http://www.frontiersin.org/microbiology/-
dc.relation.ispartofFrontiers in Microbiology-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.subjectMinION-
dc.subjectARGS-
dc.subjectfecal coliforms-
dc.subjectsewage-
dc.subjectnanopore-
dc.titleMinION Nanopore Sequencing Enables Correlation between Resistome Phenotype and Genotype of Coliform Bacteria in Municipal Sewage-
dc.typeArticle-
dc.identifier.emailDeng, Y: dengyu@hku.hk-
dc.identifier.emailLi, LG: liliguan@hku.hk-
dc.identifier.emailZhang, T: zhangt@hkucc.hku.hk-
dc.identifier.authorityZhang, T=rp00211-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.3389/fmicb.2017.02105-
dc.identifier.pmid29163399-
dc.identifier.pmcidPMC5671560-
dc.identifier.scopuseid_2-s2.0-85032565965-
dc.identifier.hkuros319389-
dc.identifier.volume8-
dc.identifier.spagearticle no. 2105-
dc.identifier.epagearticle no. 2105-
dc.identifier.isiWOS:000414012000002-
dc.publisher.placeSwitzerland-

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