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Article: Establishment of bovine expanded potential stem cells

TitleEstablishment of bovine expanded potential stem cells
Authors
Keywordsbovine
expanded potential stem cell
nuclear transfer
Issue Date2021
PublisherNational Academy of Sciences. The Journal's web site is located at http://www.pnas.org
Citation
Proceedings of the National Academy of Sciences, 2021, v. 118 n. 15, p. article no. e2018505118 How to Cite?
AbstractEmbryonic stem cells (ESCs) and induced pluripotent stem cells have the potential to differentiate to all cell types of an adult individual and are useful for studying development and for translational research. However, extrapolation of mouse and human ESC knowledge to deriving stable ESC lines of domestic ungulates and large livestock species has been challenging. In contrast to ESCs that are usually established from the blastocyst, mouse expanded potential stem cells (EPSCs) are derived from four-cell and eight-cell embryos. We have recently used the EPSC approach and established stem cells from porcine and human preimplantation embryos. EPSCs are molecularly similar across species and have broader developmental potential to generate embryonic and extraembryonic cell lineages. We further explore the EPSC technology for mammalian species refractory to the standard ESC approaches and report here the successful establishment of bovine EPSCs (bEPSCs) from preimplantation embryos of both wild-type and somatic cell nuclear transfer. bEPSCs express high levels of pluripotency genes, propagate robustly in feeder-free culture, and are genetically stable in long-term culture. bEPSCs have enriched transcriptomic features of early preimplantation embryos and differentiate in vitro to cells of the three somatic germ layers and, in chimeras, contribute to both the embryonic (fetal) and extraembryonic cell lineages. Importantly, precise gene editing is efficiently achieved in bEPSCs, and genetically modified bEPSCs can be used as donors in somatic cell nuclear transfer. bEPSCs therefore hold the potential to substantially advance biotechnology and agriculture.
DescriptionHybrid open access
Persistent Identifierhttp://hdl.handle.net/10722/299352
ISSN
2021 Impact Factor: 12.779
2020 SCImago Journal Rankings: 5.011
PubMed Central ID
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorZhao, L-
dc.contributor.authorGao, X-
dc.contributor.authorZheng, Y-
dc.contributor.authorWang, Z-
dc.contributor.authorZhao, G-
dc.contributor.authorRen, J-
dc.contributor.authorZhang, J-
dc.contributor.authorWu, J-
dc.contributor.authorWu, B-
dc.contributor.authorChen, Y-
dc.contributor.authorSun, W-
dc.contributor.authorLi, Y-
dc.contributor.authorSu, J-
dc.contributor.authorDing, Y-
dc.contributor.authorGao, Y-
dc.contributor.authorLiu, M-
dc.contributor.authorBai, X-
dc.contributor.authorSun, L-
dc.contributor.authorCao, G-
dc.contributor.authorTang, F-
dc.contributor.authorBao, S-
dc.contributor.authorLiu, P-
dc.contributor.authorLi, X-
dc.date.accessioned2021-05-10T07:00:34Z-
dc.date.available2021-05-10T07:00:34Z-
dc.date.issued2021-
dc.identifier.citationProceedings of the National Academy of Sciences, 2021, v. 118 n. 15, p. article no. e2018505118-
dc.identifier.issn0027-8424-
dc.identifier.urihttp://hdl.handle.net/10722/299352-
dc.descriptionHybrid open access-
dc.description.abstractEmbryonic stem cells (ESCs) and induced pluripotent stem cells have the potential to differentiate to all cell types of an adult individual and are useful for studying development and for translational research. However, extrapolation of mouse and human ESC knowledge to deriving stable ESC lines of domestic ungulates and large livestock species has been challenging. In contrast to ESCs that are usually established from the blastocyst, mouse expanded potential stem cells (EPSCs) are derived from four-cell and eight-cell embryos. We have recently used the EPSC approach and established stem cells from porcine and human preimplantation embryos. EPSCs are molecularly similar across species and have broader developmental potential to generate embryonic and extraembryonic cell lineages. We further explore the EPSC technology for mammalian species refractory to the standard ESC approaches and report here the successful establishment of bovine EPSCs (bEPSCs) from preimplantation embryos of both wild-type and somatic cell nuclear transfer. bEPSCs express high levels of pluripotency genes, propagate robustly in feeder-free culture, and are genetically stable in long-term culture. bEPSCs have enriched transcriptomic features of early preimplantation embryos and differentiate in vitro to cells of the three somatic germ layers and, in chimeras, contribute to both the embryonic (fetal) and extraembryonic cell lineages. Importantly, precise gene editing is efficiently achieved in bEPSCs, and genetically modified bEPSCs can be used as donors in somatic cell nuclear transfer. bEPSCs therefore hold the potential to substantially advance biotechnology and agriculture.-
dc.languageeng-
dc.publisherNational Academy of Sciences. The Journal's web site is located at http://www.pnas.org-
dc.relation.ispartofProceedings of the National Academy of Sciences-
dc.rightsProceedings of the National Academy of Sciences. Copyright © National Academy of Sciences.-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.subjectbovine-
dc.subjectexpanded potential stem cell-
dc.subjectnuclear transfer-
dc.titleEstablishment of bovine expanded potential stem cells-
dc.typeArticle-
dc.identifier.emailLiu, P: pliu88@hku.hk-
dc.identifier.authorityLiu, P=rp02328-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1073/pnas.2018505118-
dc.identifier.pmid33833056-
dc.identifier.pmcidPMC8053967-
dc.identifier.scopuseid_2-s2.0-85104165023-
dc.identifier.hkuros322426-
dc.identifier.volume118-
dc.identifier.issue15-
dc.identifier.spagearticle no. e2018505118-
dc.identifier.epagearticle no. e2018505118-
dc.identifier.isiWOS:000641176100016-
dc.publisher.placeUnited States-

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