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- Publisher Website: 10.3390/cells10071762
- Scopus: eid_2-s2.0-85114065265
- PMID: 34359932
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Article: Viral MicroRNAs Encoded by Nucleocapsid Gene of SARS-CoV-2 Are Detected during Infection, and Targeting Metabolic Pathways in Host Cells
Title | Viral MicroRNAs Encoded by Nucleocapsid Gene of SARS-CoV-2 Are Detected during Infection, and Targeting Metabolic Pathways in Host Cells |
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Authors | |
Keywords | MicroRNA SARS-CoV-2 infection host target genes cellular metabolism COVID-19 |
Issue Date | 2021 |
Publisher | MDPI AG. The Journal's web site is located at http://www.mdpi.com/journal/cells |
Citation | Cells, 2021, v. 10 n. 7, p. article no. 1762 How to Cite? |
Abstract | MicroRNAs (miRNAs) are critical regulators of gene expression that may be used to identify the pathological pathways influenced by disease and cellular interactions. Viral miRNAs (v-miRNAs) encoded by both DNA and RNA viruses induce immune dysregulation, virus production, and disease pathogenesis. Given the absence of effective treatment and the prevalence of highly infective SARS-CoV-2 strains, improved understanding of viral-associated miRNAs could provide novel mechanistic insights into the pathogenesis of COVID-19. In this study, SARS-CoV-2 v-miRNAs were identified by deep sequencing in infected Calu-3 and Vero E6 cell lines. Among the ~0.1% small RNA sequences mapped to the SARS-CoV-2 genome, the top ten SARS-CoV-2 v-miRNAs (including three encoded by the N gene; v-miRNA-N) were selected. After initial screening of conserved v-miRNA-N-28612, which was identified in both SARS-CoV and SARS-CoV-2, its expression was shown to be positively associated with viral load in COVID-19 patients. Further in silico analysis and synthetic-mimic transfection of validated SARS-CoV-2 v-miRNAs revealed novel functional targets and associations with mechanisms of cellular metabolism and biosynthesis. Our findings support the development of v-miRNA-based biomarkers and therapeutic strategies based on improved understanding of the pathophysiology of COVID-19. |
Persistent Identifier | http://hdl.handle.net/10722/308463 |
ISSN | 2021 Impact Factor: 7.666 2020 SCImago Journal Rankings: 1.220 |
PubMed Central ID | |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Meng, F | - |
dc.contributor.author | Siu, GKH | - |
dc.contributor.author | Mok, BWY | - |
dc.contributor.author | Sun, J | - |
dc.contributor.author | Fung, KSC | - |
dc.contributor.author | Lam, JYW | - |
dc.contributor.author | Wong, NK | - |
dc.contributor.author | Gedefaw, L | - |
dc.contributor.author | Luo, S | - |
dc.contributor.author | Lee, TMH | - |
dc.contributor.author | Yip, SP | - |
dc.contributor.author | Huang, CL | - |
dc.date.accessioned | 2021-12-01T07:53:41Z | - |
dc.date.available | 2021-12-01T07:53:41Z | - |
dc.date.issued | 2021 | - |
dc.identifier.citation | Cells, 2021, v. 10 n. 7, p. article no. 1762 | - |
dc.identifier.issn | 2073-4409 | - |
dc.identifier.uri | http://hdl.handle.net/10722/308463 | - |
dc.description.abstract | MicroRNAs (miRNAs) are critical regulators of gene expression that may be used to identify the pathological pathways influenced by disease and cellular interactions. Viral miRNAs (v-miRNAs) encoded by both DNA and RNA viruses induce immune dysregulation, virus production, and disease pathogenesis. Given the absence of effective treatment and the prevalence of highly infective SARS-CoV-2 strains, improved understanding of viral-associated miRNAs could provide novel mechanistic insights into the pathogenesis of COVID-19. In this study, SARS-CoV-2 v-miRNAs were identified by deep sequencing in infected Calu-3 and Vero E6 cell lines. Among the ~0.1% small RNA sequences mapped to the SARS-CoV-2 genome, the top ten SARS-CoV-2 v-miRNAs (including three encoded by the N gene; v-miRNA-N) were selected. After initial screening of conserved v-miRNA-N-28612, which was identified in both SARS-CoV and SARS-CoV-2, its expression was shown to be positively associated with viral load in COVID-19 patients. Further in silico analysis and synthetic-mimic transfection of validated SARS-CoV-2 v-miRNAs revealed novel functional targets and associations with mechanisms of cellular metabolism and biosynthesis. Our findings support the development of v-miRNA-based biomarkers and therapeutic strategies based on improved understanding of the pathophysiology of COVID-19. | - |
dc.language | eng | - |
dc.publisher | MDPI AG. The Journal's web site is located at http://www.mdpi.com/journal/cells | - |
dc.relation.ispartof | Cells | - |
dc.rights | This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License. | - |
dc.subject | MicroRNA | - |
dc.subject | SARS-CoV-2 infection | - |
dc.subject | host target genes | - |
dc.subject | cellular metabolism | - |
dc.subject | COVID-19 | - |
dc.title | Viral MicroRNAs Encoded by Nucleocapsid Gene of SARS-CoV-2 Are Detected during Infection, and Targeting Metabolic Pathways in Host Cells | - |
dc.type | Article | - |
dc.identifier.email | Mok, BWY: bobomok@hku.hk | - |
dc.description.nature | published_or_final_version | - |
dc.identifier.doi | 10.3390/cells10071762 | - |
dc.identifier.pmid | 34359932 | - |
dc.identifier.pmcid | PMC8307234 | - |
dc.identifier.scopus | eid_2-s2.0-85114065265 | - |
dc.identifier.hkuros | 330651 | - |
dc.identifier.volume | 10 | - |
dc.identifier.issue | 7 | - |
dc.identifier.spage | article no. 1762 | - |
dc.identifier.epage | article no. 1762 | - |
dc.identifier.isi | WOS:000676647800001 | - |
dc.publisher.place | Switzerland | - |