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Article: High resolution single strand conformation polymorphism analysis using formamide and ethidium bromide staining

TitleHigh resolution single strand conformation polymorphism analysis using formamide and ethidium bromide staining
Authors
Xie, THo, SLMa, OCK
KeywordsEthidium bromide
Formamide
Single strand conformation polymorphism
Issue Date1997
PublisherB M J Publishing Group. The Journal's web site is located at http://mp.bmjjournals.com/
Citation
Journal Of Clinical Pathology - Molecular Pathology, 1997, v. 50 n. 5, p. 276-278 How to Cite?
AbstractSingle strand conformation polymorphism (SSCP) analysis using ethidium bromide can be improved by adding formamide as the denaturant. This gives higher resolution than previous SSCP methods; it had 100% sensitivity in the discrimination of 14 PCR samples from two different genes, even for a long fragment close to the upper limit of 250 base pairs. This modified procedure is a rapid, simple, safe, and yet highly sensitive method for detecting structural differences in DNA fragments.
Persistent Identifierhttp://hdl.handle.net/10722/44973
ISSN
1366-8714
PubMed Central ID
PMC379648
References
References in Scopus

 

DC FieldValueLanguage
dc.contributor.authorXie, Ten_HK
dc.contributor.authorHo, SLen_HK
dc.contributor.authorMa, OCKen_HK
dc.date.accessioned2007-10-30T06:14:45Z-
dc.date.available2007-10-30T06:14:45Z-
dc.date.issued1997en_HK
dc.identifier.citationJournal Of Clinical Pathology - Molecular Pathology, 1997, v. 50 n. 5, p. 276-278en_HK
dc.identifier.issn1366-8714en_HK
dc.identifier.urihttp://hdl.handle.net/10722/44973-
dc.description.abstractSingle strand conformation polymorphism (SSCP) analysis using ethidium bromide can be improved by adding formamide as the denaturant. This gives higher resolution than previous SSCP methods; it had 100% sensitivity in the discrimination of 14 PCR samples from two different genes, even for a long fragment close to the upper limit of 250 base pairs. This modified procedure is a rapid, simple, safe, and yet highly sensitive method for detecting structural differences in DNA fragments.en_HK
dc.format.extent725012 bytes-
dc.format.extent9894 bytes-
dc.format.mimetypeapplication/pdf-
dc.format.mimetypetext/plain-
dc.languageengen_HK
dc.publisherB M J Publishing Group. The Journal's web site is located at http://mp.bmjjournals.com/en_HK
dc.relation.ispartofJournal of Clinical Pathology - Molecular Pathologyen_HK
dc.rightsMolecular Pathology. Copyright © B M J Publishing Group.en_HK
dc.subjectEthidium bromideen_HK
dc.subjectFormamideen_HK
dc.subjectSingle strand conformation polymorphismen_HK
dc.subject.meshEthidium-en_HK
dc.subject.meshFluorescent-Dyesen_HK
dc.subject.meshFormamides-en_HK
dc.subject.meshPolymorphism,-Single-Stranded-Conformationalen_HK
dc.titleHigh resolution single strand conformation polymorphism analysis using formamide and ethidium bromide stainingen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1366-8714&volume=50&issue=5&spage=276&epage=278&date=1997&atitle=High+resolution+single+strand+conformation+polymorphism+analysis+using+formamide+and+ethidium+bromide+stainingen_HK
dc.identifier.emailHo, SL:slho@hku.hken_HK
dc.identifier.authorityHo, SL=rp00240en_HK
dc.description.naturepublished_or_final_versionen_HK
dc.identifier.pmid9497922-
dc.identifier.pmcidPMC379648-
dc.identifier.scopuseid_2-s2.0-0030699217en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0030699217&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume50en_HK
dc.identifier.issue5en_HK
dc.identifier.spage276en_HK
dc.identifier.epage278en_HK
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridXie, T=17535617900en_HK
dc.identifier.scopusauthoridHo, SL=25959633500en_HK
dc.identifier.scopusauthoridMa, OCK=7004452841en_HK
dc.identifier.issnl1366-8714-

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