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- Publisher Website: 10.1128/IAI.66.5.1869-1877.1998
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- PMID: 9573063
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Article: Cytopathic effects of the major surface protein and the chymotrypsinlike protease of Treponema denticola
Title | Cytopathic effects of the major surface protein and the chymotrypsinlike protease of Treponema denticola |
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Authors | |
Issue Date | 1998 |
Publisher | American Society for Microbiology. |
Citation | Infection And Immunity, 1998, v. 66 n. 5, p. 1869-1877 How to Cite? |
Abstract | Prominent antigens of Treponema denticola have been suggested to be mediators of the cytopathic effects typically seen in periodontal disease. In the present study of the T. denticola major surface protein (Msp) and the surface-expressed chymotrypsinlike protease complex (CTLP), we characterized the ability of these proteins to adhere to and lyse epithelial cells. Msp and CTLP were closely associated in spirochete outer membranes. Purified Msp, both native and recombinant, and CTLP bound to glutaraldehyde-fixed periodontal ligament epithelial cells. Adherence of Msp was partially blocked by specific antibodies. Adherence of CTLP was partially blocked by serine protease inhibitors and was further inhibited by specific antibodies. Both native Msp and CTLP were cytotoxic toward periodontal ligament epithelial cells, and their cytotoxicity was inhibited by the same treatments that inhibited adherence. Msp, but not CTLP, lysed erythrocytes. Msp complex (partially purified outer membranes free of protease activity) was cytotoxic toward a variety of different cell types. Pore-forming activities of recombinant Msp in black lipid model membrane assays and in HeLa cell membranes were similar to those reported for the native protein, supporting the hypothesis that Msp cytotoxicity was due to its pore-forming activity. |
Persistent Identifier | http://hdl.handle.net/10722/48927 |
ISSN | 2021 Impact Factor: 3.609 2020 SCImago Journal Rankings: 1.508 |
PubMed Central ID | |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Fenno, JC | en_HK |
dc.contributor.author | Hannam, PM | en_HK |
dc.contributor.author | Leung, WK | en_HK |
dc.contributor.author | Tamura, M | en_HK |
dc.contributor.author | Uitto, VJ | en_HK |
dc.contributor.author | McBride, BC | en_HK |
dc.date.accessioned | 2008-06-12T06:29:53Z | - |
dc.date.available | 2008-06-12T06:29:53Z | - |
dc.date.issued | 1998 | en_HK |
dc.identifier.citation | Infection And Immunity, 1998, v. 66 n. 5, p. 1869-1877 | en_HK |
dc.identifier.issn | 0019-9567 | en_HK |
dc.identifier.uri | http://hdl.handle.net/10722/48927 | - |
dc.description.abstract | Prominent antigens of Treponema denticola have been suggested to be mediators of the cytopathic effects typically seen in periodontal disease. In the present study of the T. denticola major surface protein (Msp) and the surface-expressed chymotrypsinlike protease complex (CTLP), we characterized the ability of these proteins to adhere to and lyse epithelial cells. Msp and CTLP were closely associated in spirochete outer membranes. Purified Msp, both native and recombinant, and CTLP bound to glutaraldehyde-fixed periodontal ligament epithelial cells. Adherence of Msp was partially blocked by specific antibodies. Adherence of CTLP was partially blocked by serine protease inhibitors and was further inhibited by specific antibodies. Both native Msp and CTLP were cytotoxic toward periodontal ligament epithelial cells, and their cytotoxicity was inhibited by the same treatments that inhibited adherence. Msp, but not CTLP, lysed erythrocytes. Msp complex (partially purified outer membranes free of protease activity) was cytotoxic toward a variety of different cell types. Pore-forming activities of recombinant Msp in black lipid model membrane assays and in HeLa cell membranes were similar to those reported for the native protein, supporting the hypothesis that Msp cytotoxicity was due to its pore-forming activity. | en_HK |
dc.format.extent | 418 bytes | - |
dc.format.mimetype | text/html | - |
dc.language | eng | en_HK |
dc.publisher | American Society for Microbiology. | en_HK |
dc.relation.ispartof | Infection and Immunity | en_HK |
dc.rights | Infection and Immunity. Copyright © American Society for Microbiology. | en_HK |
dc.rights | Copyright © American Society for Microbiology, Infection and Immunity, 1998, v. 66 n. 5, p. 1869-1877 | en_HK |
dc.subject.mesh | Bacterial Outer Membrane Proteins - isolation & purification - toxicity | en_HK |
dc.subject.mesh | Serine Endopeptidases - toxicity | en_HK |
dc.subject.mesh | Treponema - pathogenicity | en_HK |
dc.subject.mesh | Bacterial Adhesion | en_HK |
dc.subject.mesh | CHO Cells | en_HK |
dc.title | Cytopathic effects of the major surface protein and the chymotrypsinlike protease of Treponema denticola | en_HK |
dc.type | Article | en_HK |
dc.identifier.openurl | http://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0019-9567&volume=66&issue=5&spage=1869&epage=1877&date=1998&atitle=Cytopathic+effects+of+the+major+surface+protein+and+the+chymotrypsinlike+protease+of+Treponema+denticola | en_HK |
dc.identifier.email | Leung, WK:ewkleung@hkucc.hku.hk | en_HK |
dc.identifier.authority | Leung, WK=rp00019 | en_HK |
dc.description.nature | published_or_final_version | en_HK |
dc.identifier.doi | 10.1128/IAI.66.5.1869-1877.1998 | - |
dc.identifier.pmid | 9573063 | - |
dc.identifier.pmcid | PMC108137 | - |
dc.identifier.scopus | eid_2-s2.0-0031900968 | en_HK |
dc.identifier.hkuros | 32924 | - |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-0031900968&selection=ref&src=s&origin=recordpage | en_HK |
dc.identifier.volume | 66 | en_HK |
dc.identifier.issue | 5 | en_HK |
dc.identifier.spage | 1869 | en_HK |
dc.identifier.epage | 1877 | en_HK |
dc.identifier.isi | WOS:000073413100009 | - |
dc.publisher.place | United States | en_HK |
dc.identifier.scopusauthorid | Fenno, JC=6602072985 | en_HK |
dc.identifier.scopusauthorid | Hannam, PM=6602614873 | en_HK |
dc.identifier.scopusauthorid | Leung, WK=25224691800 | en_HK |
dc.identifier.scopusauthorid | Tamura, M=8773768700 | en_HK |
dc.identifier.scopusauthorid | Uitto, VJ=7004455896 | en_HK |
dc.identifier.scopusauthorid | McBride, BC=7102465580 | en_HK |
dc.identifier.issnl | 0019-9567 | - |