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Article: Transgenic mice expressing Cre-recombinase specifically in retinal rod bipolar neurons

TitleTransgenic mice expressing Cre-recombinase specifically in retinal rod bipolar neurons
Authors
Zhang, XMChen, BYNg, AHLTanner, JATay, DSo, KFRachel, RACopeland, NGJenkins, NAHuang, JD
Issue Date2005
PublisherAssociation for Research in Vision and Ophthalmology. The Journal's web site is located at http://www.iovs.org
Citation
Investigative Ophthalmology And Visual Science, 2005, v. 46 n. 10, p. 3515-3520 How to Cite?
DOI: http://dx.doi.org/10.1167/iovs.04-1201
AbstractPURPOSE. To establish a transgenic mouse line that expresses Cre-recombinase in retinal rod bipolar cells for the generation of rod bipolar cell-specific knockout mutants. METHODS. The IRES-Cre-cDNA. fragment was inserted into a 173-kb bacterial artificial chromosome (BAC) carrying the intact Pcp2 gene, by using red-mediated recombineering. Transgenic mice were generated with the modified BAC and identified. The Cre-transgenic mice were crossed with ROSA26 and Z/EG reporter mice to detect Cre-recombinase activity. RESULTS. X-gal staining showed that strong Cre-recombinase activities were present in retinal inner nuclear layers and cerebellar Purkinje cells. Double staining with an anti-GFP antibody and an anti-PKCα antibody (specific for retinal rod bipolar cells) revealed that Cre-recombinase activity localized exclusively to the rod bipolar cells in the retina. CONCLUSIONS. A mouse BAC-Pcp2-IRES-Cre transgenic line that expresses Cre-recombinase in retinal rod bipolar neurons has been established. Because mutations in some ubiquitously expressed genes may result in retinal degenerative diseases, the mouse strain BAC-Pcp2-IRES-Cre will be a useful new tool for investigating the effects of retinal rod bipolar cell-specific gene inactivation. Copyright © Association for Research in Vision and Ophthalmology.
Persistent Identifierhttp://hdl.handle.net/10722/67627
ISSN
0146-0404
2021 Impact Factor: 4.925
2020 SCImago Journal Rankings: 1.935
ISI Accession Number ID
WOS:000232112900009
References
References in Scopus

 

DC FieldValueLanguage
dc.contributor.authorZhang, XMen_HK
dc.contributor.authorChen, BYen_HK
dc.contributor.authorNg, AHLen_HK
dc.contributor.authorTanner, JAen_HK
dc.contributor.authorTay, Den_HK
dc.contributor.authorSo, KFen_HK
dc.contributor.authorRachel, RAen_HK
dc.contributor.authorCopeland, NGen_HK
dc.contributor.authorJenkins, NAen_HK
dc.contributor.authorHuang, JDen_HK
dc.date.accessioned2010-09-06T05:56:49Z-
dc.date.available2010-09-06T05:56:49Z-
dc.date.issued2005en_HK
dc.identifier.citationInvestigative Ophthalmology And Visual Science, 2005, v. 46 n. 10, p. 3515-3520en_HK
dc.identifier.issn0146-0404en_HK
dc.identifier.urihttp://hdl.handle.net/10722/67627-
dc.description.abstractPURPOSE. To establish a transgenic mouse line that expresses Cre-recombinase in retinal rod bipolar cells for the generation of rod bipolar cell-specific knockout mutants. METHODS. The IRES-Cre-cDNA. fragment was inserted into a 173-kb bacterial artificial chromosome (BAC) carrying the intact Pcp2 gene, by using red-mediated recombineering. Transgenic mice were generated with the modified BAC and identified. The Cre-transgenic mice were crossed with ROSA26 and Z/EG reporter mice to detect Cre-recombinase activity. RESULTS. X-gal staining showed that strong Cre-recombinase activities were present in retinal inner nuclear layers and cerebellar Purkinje cells. Double staining with an anti-GFP antibody and an anti-PKCα antibody (specific for retinal rod bipolar cells) revealed that Cre-recombinase activity localized exclusively to the rod bipolar cells in the retina. CONCLUSIONS. A mouse BAC-Pcp2-IRES-Cre transgenic line that expresses Cre-recombinase in retinal rod bipolar neurons has been established. Because mutations in some ubiquitously expressed genes may result in retinal degenerative diseases, the mouse strain BAC-Pcp2-IRES-Cre will be a useful new tool for investigating the effects of retinal rod bipolar cell-specific gene inactivation. Copyright © Association for Research in Vision and Ophthalmology.en_HK
dc.languageengen_HK
dc.publisherAssociation for Research in Vision and Ophthalmology. The Journal's web site is located at http://www.iovs.orgen_HK
dc.relation.ispartofInvestigative Ophthalmology and Visual Scienceen_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshChromosomes, Artificial, Bacterialen_HK
dc.subject.meshFemaleen_HK
dc.subject.meshFluorescent Antibody Technique, Indirecten_HK
dc.subject.meshGalactosides - metabolismen_HK
dc.subject.meshGreen Fluorescent Proteins - genetics - metabolismen_HK
dc.subject.meshGuanine Nucleotide Exchange Factorsen_HK
dc.subject.meshIndoles - metabolismen_HK
dc.subject.meshIntegrases - genetics - metabolismen_HK
dc.subject.meshInterneurons - enzymologyen_HK
dc.subject.meshMaleen_HK
dc.subject.meshMiceen_HK
dc.subject.meshMice, Inbred C3Hen_HK
dc.subject.meshMice, Inbred C57BLen_HK
dc.subject.meshMice, Knockouten_HK
dc.subject.meshMice, Transgenicen_HK
dc.subject.meshNeuropeptides - geneticsen_HK
dc.subject.meshPregnancyen_HK
dc.subject.meshPurkinje Cells - enzymologyen_HK
dc.subject.meshRetinal Rod Photoreceptor Cells - cytology - embryologyen_HK
dc.subject.meshbeta-Galactosidase - metabolismen_HK
dc.titleTransgenic mice expressing Cre-recombinase specifically in retinal rod bipolar neuronsen_HK
dc.typeArticleen_HK
dc.identifier.emailTanner, JA:jatanner@hku.hken_HK
dc.identifier.emailTay, D:dkctay@hkucc.hku.hken_HK
dc.identifier.emailSo, KF:hrmaskf@hkucc.hku.hken_HK
dc.identifier.emailHuang, JD:jdhuang@hkucc.hku.hken_HK
dc.identifier.authorityTanner, JA=rp00495en_HK
dc.identifier.authorityTay, D=rp00336en_HK
dc.identifier.authoritySo, KF=rp00329en_HK
dc.identifier.authorityHuang, JD=rp00451en_HK
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1167/iovs.04-1201en_HK
dc.identifier.pmid16186328en_HK
dc.identifier.scopuseid_2-s2.0-32944457757en_HK
dc.identifier.hkuros107078en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-32944457757&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume46en_HK
dc.identifier.issue10en_HK
dc.identifier.spage3515en_HK
dc.identifier.epage3520en_HK
dc.identifier.isiWOS:000232112900009-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridZhang, XM=7410273116en_HK
dc.identifier.scopusauthoridChen, BY=14051424300en_HK
dc.identifier.scopusauthoridNg, AHL=12445077000en_HK
dc.identifier.scopusauthoridTanner, JA=35513993000en_HK
dc.identifier.scopusauthoridTay, D=7006796825en_HK
dc.identifier.scopusauthoridSo, KF=34668391300en_HK
dc.identifier.scopusauthoridRachel, RA=7003812116en_HK
dc.identifier.scopusauthoridCopeland, NG=35374759300en_HK
dc.identifier.scopusauthoridJenkins, NA=35379887700en_HK
dc.identifier.scopusauthoridHuang, JD=8108660600en_HK
dc.identifier.issnl0146-0404-

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