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Article: Preparative isolation and purification of astaxanthin from the microalga Chlorococcum sp. by high-speed counter-current chromatography

TitlePreparative isolation and purification of astaxanthin from the microalga Chlorococcum sp. by high-speed counter-current chromatography
Authors
Li, HBChen, F
KeywordsAntioxidants
Astaxanthin
Carotenoids
Chlorococcum sp.
Counter-current chromatography
Issue Date2001
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/chroma
Citation
Journal Of Chromatography A, 2001, v. 925 n. 1-2, p. 133-137 How to Cite?
DOI: http://dx.doi.org/10.1016/S0021-9673(01)01022-6
AbstractHigh-speed counter-current chromatography was applied to the isolation and purification of astaxanthin from microalgae. The crude astaxanthin was obtained by extraction with organic solvents after the astaxanthin esters were saponified. Preparative high-speed counter-current chromatography with a two-phase solvent system composed of n-hexane-ethyl acetate-ethanol-water (5:5:6.5:3, v/v) was successfully performed yielding astaxanthin at 97% purity from 250 mg of the crude extract in a one-step separation. Copyright © 2001 Elsevier Science B.V.
Persistent Identifierhttp://hdl.handle.net/10722/68367
ISSN
0021-9673
2021 Impact Factor: 4.601
2020 SCImago Journal Rankings: 1.011
ISI Accession Number ID
WOS:000170432100013
References
References in Scopus

 

DC FieldValueLanguage
dc.contributor.authorLi, HBen_HK
dc.contributor.authorChen, Fen_HK
dc.date.accessioned2010-09-06T06:03:59Z-
dc.date.available2010-09-06T06:03:59Z-
dc.date.issued2001en_HK
dc.identifier.citationJournal Of Chromatography A, 2001, v. 925 n. 1-2, p. 133-137en_HK
dc.identifier.issn0021-9673en_HK
dc.identifier.urihttp://hdl.handle.net/10722/68367-
dc.description.abstractHigh-speed counter-current chromatography was applied to the isolation and purification of astaxanthin from microalgae. The crude astaxanthin was obtained by extraction with organic solvents after the astaxanthin esters were saponified. Preparative high-speed counter-current chromatography with a two-phase solvent system composed of n-hexane-ethyl acetate-ethanol-water (5:5:6.5:3, v/v) was successfully performed yielding astaxanthin at 97% purity from 250 mg of the crude extract in a one-step separation. Copyright © 2001 Elsevier Science B.V.en_HK
dc.languageengen_HK
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/chromaen_HK
dc.relation.ispartofJournal of Chromatography Aen_HK
dc.rightsJournal of Chromatography A. Copyright © Elsevier BV.en_HK
dc.subjectAntioxidantsen_HK
dc.subjectAstaxanthinen_HK
dc.subjectCarotenoidsen_HK
dc.subjectChlorococcum sp.en_HK
dc.subjectCounter-current chromatographyen_HK
dc.titlePreparative isolation and purification of astaxanthin from the microalga Chlorococcum sp. by high-speed counter-current chromatographyen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0021-9673&volume=925&spage=133&epage=137&date=2001&atitle=Preparative+isolation+and+purification+of+astaxanthin+from+the+microalga+Chlorococcum+sp.+by+high-speed+counter-current+chromatographyen_HK
dc.identifier.emailChen, F: sfchen@hku.hken_HK
dc.identifier.authorityChen, F=rp00672en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/S0021-9673(01)01022-6en_HK
dc.identifier.pmid11519799-
dc.identifier.scopuseid_2-s2.0-0035800701en_HK
dc.identifier.hkuros72676en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0035800701&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume925en_HK
dc.identifier.issue1-2en_HK
dc.identifier.spage133en_HK
dc.identifier.epage137en_HK
dc.identifier.isiWOS:000170432100013-
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridLi, HB=25649944400en_HK
dc.identifier.scopusauthoridChen, F=7404907980en_HK
dc.identifier.issnl0021-9673-

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