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Article: Preparative high-speed counter-current chromatography for purification of shikonin from the Chinese medicinal plant Lithospermum erythrorhizon

TitlePreparative high-speed counter-current chromatography for purification of shikonin from the Chinese medicinal plant Lithospermum erythrorhizon
Authors
Lu, HTJiang, YChen, F
KeywordsCounter-current chromatography
Lithospermum erythrorhizon
Pharmaceutical analysis
Plant materials
Preparative chromatography
Shikonin
Issue Date2004
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/chroma
Citation
Journal Of Chromatography A, 2004, v. 1023 n. 1, p. 159-163 How to Cite?
DOI: http://dx.doi.org/10.1016/j.chroma.2003.09.022
AbstractThe bioactive compound shikonin was successfully isolated and purified from the crude extract of the traditional Chinese medicinal plant Lithospermum erythrorhizon Sieb. et Zucc. by preparative high-speed counter-current chromatography (HSCCC). The preparative HSCCC was performed using a two-phase solvent system composed of n-hexane-ethylacetate-ethanol-water (16:14:14:5 (v/v)). A total amount of 19.6mg of shikonin at 98.9% purity was obtained from 52mg of the crude extract (containing 38.9% shikonin) with 96.9% recovery. The preparative isolation and purification of shikonin by HSCCC was completed in 200min in a one-step separation. © 2003 Elsevier B.V. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/68435
ISSN
0021-9673
2021 Impact Factor: 4.601
2020 SCImago Journal Rankings: 1.011
ISI Accession Number ID
WOS:000187719900017
References
References in Scopus

 

DC FieldValueLanguage
dc.contributor.authorLu, HTen_HK
dc.contributor.authorJiang, Yen_HK
dc.contributor.authorChen, Fen_HK
dc.date.accessioned2010-09-06T06:04:35Z-
dc.date.available2010-09-06T06:04:35Z-
dc.date.issued2004en_HK
dc.identifier.citationJournal Of Chromatography A, 2004, v. 1023 n. 1, p. 159-163en_HK
dc.identifier.issn0021-9673en_HK
dc.identifier.urihttp://hdl.handle.net/10722/68435-
dc.description.abstractThe bioactive compound shikonin was successfully isolated and purified from the crude extract of the traditional Chinese medicinal plant Lithospermum erythrorhizon Sieb. et Zucc. by preparative high-speed counter-current chromatography (HSCCC). The preparative HSCCC was performed using a two-phase solvent system composed of n-hexane-ethylacetate-ethanol-water (16:14:14:5 (v/v)). A total amount of 19.6mg of shikonin at 98.9% purity was obtained from 52mg of the crude extract (containing 38.9% shikonin) with 96.9% recovery. The preparative isolation and purification of shikonin by HSCCC was completed in 200min in a one-step separation. © 2003 Elsevier B.V. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/chromaen_HK
dc.relation.ispartofJournal of Chromatography Aen_HK
dc.rightsJournal of Chromatography A. Copyright © Elsevier BV.en_HK
dc.subjectCounter-current chromatographyen_HK
dc.subjectLithospermum erythrorhizonen_HK
dc.subjectPharmaceutical analysisen_HK
dc.subjectPlant materialsen_HK
dc.subjectPreparative chromatographyen_HK
dc.subjectShikoninen_HK
dc.titlePreparative high-speed counter-current chromatography for purification of shikonin from the Chinese medicinal plant Lithospermum erythrorhizonen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0021-9673&volume=1023&issue=1&spage=159&epage=163&date=2004&atitle=Preparative+high-speed+counter-current+chromatography+for+purification+of+shikonin+from+the+Chinese+medicinal+plant+Lithospermum+erythrorhizonen_HK
dc.identifier.emailChen, F: sfchen@hku.hken_HK
dc.identifier.authorityChen, F=rp00672en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.chroma.2003.09.022en_HK
dc.identifier.pmid14760860-
dc.identifier.scopuseid_2-s2.0-0344256459en_HK
dc.identifier.hkuros90923en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0344256459&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume1023en_HK
dc.identifier.issue1en_HK
dc.identifier.spage159en_HK
dc.identifier.epage163en_HK
dc.identifier.isiWOS:000187719900017-
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridLu, HT=7404843255en_HK
dc.identifier.scopusauthoridJiang, Y=24605346600en_HK
dc.identifier.scopusauthoridChen, F=7404907980en_HK
dc.identifier.issnl0021-9673-

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