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Article: Determination of vitamin B12 in pharmaceutical preparations by a highly sensitive fluorimetric method

TitleDetermination of vitamin B12 in pharmaceutical preparations by a highly sensitive fluorimetric method
Authors
Li, HBChen, F
Issue Date2000
PublisherSpringer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00216/index.htm
Citation
Fresenius' Journal Of Analytical Chemistry, 2000, v. 368 n. 8, p. 836-838 How to Cite?
DOI: http://dx.doi.org/10.1007/s002160000595
AbstractA fluorimetric method for the determination of vitamin B12 has been developed. The fluorescence emission was measured at λex/λem275/305 nm in phosphate buffer solution (pH 7.0), and the experimental variables and possible interference were studied. The linear calibration range was 1.000 ng/mL to 100.0 ng/mL with a correlation coefficient of 0.9994 and a detection limit of 0.1 ng/mL. The method is rapid, simple and highly sensitive. It was used to determine vitamin B12 in pharmaceutical preparations. The recovery was 96%-98% and the relative standard deviation was in the range of 1.8%-2.7%. The results agreed with those obtained by spectrophotometry. © Springer-Verlag 2000.
Persistent Identifierhttp://hdl.handle.net/10722/68718
ISSN
0937-0633
ISI Accession Number ID
WOS:000165896100016
References
References in Scopus

 

DC FieldValueLanguage
dc.contributor.authorLi, HBen_HK
dc.contributor.authorChen, Fen_HK
dc.date.accessioned2010-09-06T06:07:02Z-
dc.date.available2010-09-06T06:07:02Z-
dc.date.issued2000en_HK
dc.identifier.citationFresenius' Journal Of Analytical Chemistry, 2000, v. 368 n. 8, p. 836-838en_HK
dc.identifier.issn0937-0633en_HK
dc.identifier.urihttp://hdl.handle.net/10722/68718-
dc.description.abstractA fluorimetric method for the determination of vitamin B12 has been developed. The fluorescence emission was measured at λex/λem275/305 nm in phosphate buffer solution (pH 7.0), and the experimental variables and possible interference were studied. The linear calibration range was 1.000 ng/mL to 100.0 ng/mL with a correlation coefficient of 0.9994 and a detection limit of 0.1 ng/mL. The method is rapid, simple and highly sensitive. It was used to determine vitamin B12 in pharmaceutical preparations. The recovery was 96%-98% and the relative standard deviation was in the range of 1.8%-2.7%. The results agreed with those obtained by spectrophotometry. © Springer-Verlag 2000.en_HK
dc.languageengen_HK
dc.publisherSpringer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00216/index.htmen_HK
dc.relation.ispartofFresenius' Journal of Analytical Chemistryen_HK
dc.titleDetermination of vitamin B12 in pharmaceutical preparations by a highly sensitive fluorimetric methoden_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0937-0633&volume=368&issue=8&spage=836&epage=838&date=2000&atitle=Determination+of+vitamin+B12+in+pharmaceutical+preparations+by+a+highly+sensitive+fluorimetric+methoden_HK
dc.identifier.emailChen, F: sfchen@hku.hken_HK
dc.identifier.authorityChen, F=rp00672en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1007/s002160000595-
dc.identifier.pmid11227572-
dc.identifier.scopuseid_2-s2.0-0034572919en_HK
dc.identifier.hkuros61498en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0034572919&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume368en_HK
dc.identifier.issue8en_HK
dc.identifier.spage836en_HK
dc.identifier.epage838en_HK
dc.identifier.isiWOS:000165896100016-
dc.publisher.placeGermanyen_HK
dc.identifier.scopusauthoridLi, HB=25649944400en_HK
dc.identifier.scopusauthoridChen, F=7404907980en_HK
dc.identifier.issnl0937-0633-

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