Determination of dioxopromethazine hydrochloride by capillary electrophoresis with electrochemiluminescence detection

Abstract

The paper presents a rapid method for the determination of dioxopromethazine hydrochloride (DPZ), an antihistamine drug, by the capillary electrophoresis with electrochemiluminescene detection (CE-ECL) using tris(2,2′-bipyridyl)ruthenium(II) (Ru(bpy)3 2+) reagent. This CE-ECL detection method has high sensitivity, good selectivity and reproducibility for DPZ analysis. Under the optimized conditions: separation capillary, 38 cm length (25 μm i.d.); sample injection, 10 s at 8 kV; separation voltage, 12.5 kV; running buffer, 20 mmol L-1 sodium phosphate of pH 6.0; detection potential, 1.15 V; 50 mmol L-1 of phosphate buffer (pH 7.14) containing 5 mmol L-1 of Ru(bpy) 3 2+ in ECL detection cell, the detection limit of DPZ was 0.05 μmol L-1 (S/N = 3). The linear range extended from 5 to 100 μmol L-1. The linear curve obtained was Y = 181.62 + 9.28X with a correlation coefficient of 0.9970. The relative standard deviations of the ECL intensity and the migration time for six continuous injections of 5 μmol L-1 DPZ were 3.7% and 0.92%, respectively. The CE-ECL method was applied to analyze DPZ in real samples including tablets, rat serum and human urine, and satisfactory results were obtained without interference from samples matrix. The CE-ECL technique was proved to be a potential method for the detection of DPZ in clinic analysis. © 2005 Elsevier B.V. All rights reserved.