Degradation of purified terephthalic acid and expression of mnp gene for GEM Fhhh

Abstract

The levels of the specific degradation rate (SDR) for the genetically engineered microorganism (GEM) Fhhh from inter-kingdom protoplast fusion and the parental strain \%Phanerochaete chrysosporium\% (PC) in degradation of purified terephthalic acid (PTA) were affected by (NH-4)-2C-4H-4O-6,H-2O-2,Mn+{2+},and pH value.Except pH value,the other 3 factors affecting the SDR levels had a same order for both strains.The pH value in the order was at the first site for Fhhh,and that for PC at the third site.At the optimal levels,the SDR value of Fhhh was 11^11% higher than that of PC;the value of manganese peroxidase specific activity (MnP|SA) of Fhhh was 15^22% higher than that of PC.There was a positive relationship between SDR and MnP|SA significantly.The optimal levels of 4 factors for SDR also were the optimal levels for mnp gene expression.The technique of the mnp gene expression control would be useful to treat PTA wastewater.