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Article: 12-Lipoxygenase inhibition induced apoptosis in human gastric cancer cells

Title12-Lipoxygenase inhibition induced apoptosis in human gastric cancer cells
Authors
Wong, BCYWang, WPCho, CHFan, XMLin, MCMKung, HFLam, SK
Issue Date2001
PublisherOxford University Press. The Journal's web site is located at http://carcin.oxfordjournals.org/
Citation
Carcinogenesis, 2001, v. 22 n. 9, p. 1349-1354 How to Cite?
DOI: http://dx.doi.org/10.1093/carcin/22.9.1349
AbstractArachidonic acid release from membrane phospholipids is essential for tumour cell proliferation. Lipoxygenases constitute a pathway for arachidonate metabolism. The present study investigated the expression of 12-lipoxygenase and its effect on cell proliferation as well as survival in two human gastric cancer cell lines (AGS and MKN-28). RT-PCR and western blots, respectively, showed 12-LOX mRNA and protein expression in both AGS and MKN-28 cell lines. Treatment with a 12-LOX inhibitor, baicalein, significantly inhibited cancer cell proliferation, but a metabolite of 12-LOX activity, 12 hydroxyeicosatetraenoic acid (12-HETE) reversed baicalein-induced growth inhibition. Furthermore, the blockade of the 12-LOX pathway through a 12-LOX inhibitor and antisense induced apoptosis of gastric cancer cell lines. The biochemical characteristics of apoptosis were p53-independent combined with a decrease in bcl-2 expression. Caspase-7 was proteolytically activated and responsible for the apoptosis execution.
Persistent Identifierhttp://hdl.handle.net/10722/76285
ISSN
0143-3334
2021 Impact Factor: 4.741
2020 SCImago Journal Rankings: 1.688
ISI Accession Number ID
WOS:000171021100003
References
References in Scopus

 

DC FieldValueLanguage
dc.contributor.authorWong, BCYen_HK
dc.contributor.authorWang, WPen_HK
dc.contributor.authorCho, CHen_HK
dc.contributor.authorFan, XMen_HK
dc.contributor.authorLin, MCMen_HK
dc.contributor.authorKung, HFen_HK
dc.contributor.authorLam, SKen_HK
dc.date.accessioned2010-09-06T07:19:36Z-
dc.date.available2010-09-06T07:19:36Z-
dc.date.issued2001en_HK
dc.identifier.citationCarcinogenesis, 2001, v. 22 n. 9, p. 1349-1354en_HK
dc.identifier.issn0143-3334en_HK
dc.identifier.urihttp://hdl.handle.net/10722/76285-
dc.description.abstractArachidonic acid release from membrane phospholipids is essential for tumour cell proliferation. Lipoxygenases constitute a pathway for arachidonate metabolism. The present study investigated the expression of 12-lipoxygenase and its effect on cell proliferation as well as survival in two human gastric cancer cell lines (AGS and MKN-28). RT-PCR and western blots, respectively, showed 12-LOX mRNA and protein expression in both AGS and MKN-28 cell lines. Treatment with a 12-LOX inhibitor, baicalein, significantly inhibited cancer cell proliferation, but a metabolite of 12-LOX activity, 12 hydroxyeicosatetraenoic acid (12-HETE) reversed baicalein-induced growth inhibition. Furthermore, the blockade of the 12-LOX pathway through a 12-LOX inhibitor and antisense induced apoptosis of gastric cancer cell lines. The biochemical characteristics of apoptosis were p53-independent combined with a decrease in bcl-2 expression. Caspase-7 was proteolytically activated and responsible for the apoptosis execution.en_HK
dc.languageengen_HK
dc.publisherOxford University Press. The Journal's web site is located at http://carcin.oxfordjournals.org/en_HK
dc.relation.ispartofCarcinogenesisen_HK
dc.rightsCarcinogenesis. Copyright © Oxford University Press.en_HK
dc.subject.mesh12-Hydroxy-5,8,10,14-eicosatetraenoic Acid - pharmacologyen_HK
dc.subject.meshApoptosis - drug effects - physiologyen_HK
dc.subject.meshArachidonate 12-Lipoxygenase - biosynthesis - geneticsen_HK
dc.subject.meshBlood Platelets - enzymologyen_HK
dc.subject.meshBlotting, Westernen_HK
dc.subject.meshCaspase 3en_HK
dc.subject.meshCaspase 7en_HK
dc.subject.meshCaspases - metabolismen_HK
dc.subject.meshCell Division - drug effects - physiologyen_HK
dc.subject.meshDrug Interactionsen_HK
dc.subject.meshFlavanonesen_HK
dc.subject.meshFlavonoids - pharmacologyen_HK
dc.subject.meshGene Expression Regulation, Neoplastic - drug effectsen_HK
dc.subject.meshGrowth Inhibitors - pharmacologyen_HK
dc.subject.meshHumansen_HK
dc.subject.meshLipoxygenase Inhibitors - pharmacologyen_HK
dc.subject.meshRNA, Messenger - biosynthesis - geneticsen_HK
dc.subject.meshStomach Neoplasms - enzymology - pathologyen_HK
dc.subject.meshTumor Cells, Cultureden_HK
dc.title12-Lipoxygenase inhibition induced apoptosis in human gastric cancer cellsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0143-3334&volume=22&issue=9&spage=1349&epage=54&date=2001&atitle=12-Lipoxygenase+inhibition+induced+apoptosis+in+human+gastric+cancer+cellsen_HK
dc.identifier.emailWong, BCY:bcywong@hku.hken_HK
dc.identifier.emailLin, MCM:mcllin@hkucc.hku.hken_HK
dc.identifier.authorityWong, BCY=rp00429en_HK
dc.identifier.authorityLin, MCM=rp00746en_HK
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1093/carcin/22.9.1349-
dc.identifier.pmid11532854-
dc.identifier.scopuseid_2-s2.0-0034811654en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0034811654&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume22en_HK
dc.identifier.issue9en_HK
dc.identifier.spage1349en_HK
dc.identifier.epage1354en_HK
dc.identifier.isiWOS:000171021100003-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridWong, BCY=7402023340en_HK
dc.identifier.scopusauthoridWang, WP=7501765704en_HK
dc.identifier.scopusauthoridCho, CH=14067000400en_HK
dc.identifier.scopusauthoridFan, XM=35187111100en_HK
dc.identifier.scopusauthoridLin, MCM=7404816359en_HK
dc.identifier.scopusauthoridKung, HF=7402514190en_HK
dc.identifier.scopusauthoridLam, SK=7402279473en_HK
dc.identifier.issnl0143-3334-

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