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Book Chapter: Preparative isolation and purification of astaxanthin from the green microalga Chlorococcum sp. by high-speed counter-current chromatography
Title | Preparative isolation and purification of astaxanthin from the green microalga Chlorococcum sp. by high-speed counter-current chromatography |
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Authors | |
Keywords | Peak Resolution Gradient Mode Crude Sample Preparative Isolation Astaxanthin Ester |
Issue Date | 2001 |
Publisher | Springer. |
Citation | Preparative isolation and purification of astaxanthin from the green microalga Chlorococcum sp by high-speed counter-current chromatography. In Chen, F & Jiang, Y (Eds.). Algae and their Biotechnological Potential, p. 127-134. The Netherlands: Springer, 2001 How to Cite? |
Abstract | High-speed counter-current chromatography was applied to the isolation and purification of astaxanthin from microalgae. The crude astaxanthin was obtained by extraction with organic solvents after the astaxanthin esters were saponified. Preparative high-speed counter-current chromatography with a two-phase solvent system composed of n-hexane-ethyl acetate-ethanol-water (5:5:6.5:3, v/v) was successfully performed yielding astaxanthin at 97 % purity from 250 mg of the crude extract in a one-step separation. |
Persistent Identifier | http://hdl.handle.net/10722/123207 |
ISBN |
DC Field | Value | Language |
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dc.contributor.author | Li, H | en_HK |
dc.contributor.author | Chen, SF | en_HK |
dc.date.accessioned | 2010-09-26T11:55:28Z | - |
dc.date.available | 2010-09-26T11:55:28Z | - |
dc.date.issued | 2001 | en_HK |
dc.identifier.citation | Preparative isolation and purification of astaxanthin from the green microalga Chlorococcum sp by high-speed counter-current chromatography. In Chen, F & Jiang, Y (Eds.). Algae and their Biotechnological Potential, p. 127-134. The Netherlands: Springer, 2001 | en_HK |
dc.identifier.isbn | 978-1-4020-0162-8 | - |
dc.identifier.uri | http://hdl.handle.net/10722/123207 | - |
dc.description.abstract | High-speed counter-current chromatography was applied to the isolation and purification of astaxanthin from microalgae. The crude astaxanthin was obtained by extraction with organic solvents after the astaxanthin esters were saponified. Preparative high-speed counter-current chromatography with a two-phase solvent system composed of n-hexane-ethyl acetate-ethanol-water (5:5:6.5:3, v/v) was successfully performed yielding astaxanthin at 97 % purity from 250 mg of the crude extract in a one-step separation. | - |
dc.language | eng | en_HK |
dc.publisher | Springer. | en_HK |
dc.relation.ispartof | Algae and their Biotechnological Potential | en_HK |
dc.rights | Algae and Their Biotechnological Potential. Copyright © Kluwer Academic Publishers. | en_HK |
dc.subject | Peak Resolution | - |
dc.subject | Gradient Mode | - |
dc.subject | Crude Sample | - |
dc.subject | Preparative Isolation | - |
dc.subject | Astaxanthin Ester | - |
dc.title | Preparative isolation and purification of astaxanthin from the green microalga Chlorococcum sp. by high-speed counter-current chromatography | en_HK |
dc.type | Book_Chapter | en_HK |
dc.identifier.email | Li, H: hbli2000@yahoo.com | en_HK |
dc.identifier.email | Chen, SF: sfchen@hkusua.hku.hk | en_HK |
dc.identifier.authority | Chen, SF=rp00672 | en_HK |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1007/978-94-015-9835-4_9 | - |
dc.identifier.hkuros | 72826 | en_HK |
dc.identifier.epage | 134 | en_HK |