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Article: Effects of silver diamine fluoride on dentine carious lesions induced by Streptococcus mutans and Actinomyces naeslundii biofilms
Title | Effects of silver diamine fluoride on dentine carious lesions induced by Streptococcus mutans and Actinomyces naeslundii biofilms | ||||
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Authors | |||||
Issue Date | 2012 | ||||
Publisher | Wiley-Blackwell Publishing Ltd.. The Journal's web site is located at http://www.wiley.com/bw/journal.asp?ref=0960-7439 | ||||
Citation | International Journal Of Paediatric Dentistry, 2012, v. 22 n. 1, p. 2-10 How to Cite? | ||||
Abstract | Background. Silver diamine fluoride (SDF) has been shown to be a successful treatment for arresting caries. However, the mechanism of SDF is to be elucidated. Aim. To characterize the effects of SDF on dentine carious induced by Streptococcus mutans and Actinomyces naeslundii. Design. Thirty-two artificially demineralized human dentine blocks were inoculated: 16 with S. mutans and 16 with A. naeslundii. Either SDF or water was applied to eight blocks in each group. Biofilm morphology, microbial kinetics and viability were evaluated by scanning electron microscopy, colony forming units, and confocal microscopy. The crosssection of the dentine carious lesions were assessed by microhardness testing, scanning electron microscopy with energy-dispersive x-ray spectroscopy and Fourier transform infrared spectroscopy. Results. Biofilm counts were reduced in SDF group than control (P<0.01). Surfaces of carious lesions were harder after SDF application than after water application (P<0.05), in S. mutans group, Ca and P weight percentage after SDF application than after water application (P<0.05). Lesions showed a significantly reduced level of matrix to phosphate after SDF treatment (P<0.05). Conclusion. Present study showed that SDF posses an anti-microbial activity against cariogenic biofilm of S. mutans or A. naeslundii formed on dentine surfaces. SDF slowed down demineralization of dentine. This dual activity could be the reason behind clinical success of SDF. © 2011 The Authors. International Journal of Paediatric Dentistry © 2011 BSPD, IAPD and Blackwell Publishing Ltd. | ||||
Persistent Identifier | http://hdl.handle.net/10722/137184 | ||||
ISSN | 2023 Impact Factor: 2.3 2023 SCImago Journal Rankings: 0.885 | ||||
ISI Accession Number ID |
Funding Information: The authors thank Ms Joyce Yau for assistance in the microbiological work, Mr Simon Lee for support in the SEM study, Dr Borong Shi for assistance in FTIR analysis, and Dr Trevor Lane for editorial assistance. This study was supported by Grant No. 200802159003 of the Seed Fund for Basic Research, The University of Hong Kong. | ||||
References | |||||
Grants |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Chu, CH | en_HK |
dc.contributor.author | Mei, L | en_HK |
dc.contributor.author | Seneviratne, CJ | en_HK |
dc.contributor.author | Lo, ECM | en_HK |
dc.date.accessioned | 2011-08-26T14:18:07Z | - |
dc.date.available | 2011-08-26T14:18:07Z | - |
dc.date.issued | 2012 | en_HK |
dc.identifier.citation | International Journal Of Paediatric Dentistry, 2012, v. 22 n. 1, p. 2-10 | en_HK |
dc.identifier.issn | 0960-7439 | en_HK |
dc.identifier.uri | http://hdl.handle.net/10722/137184 | - |
dc.description.abstract | Background. Silver diamine fluoride (SDF) has been shown to be a successful treatment for arresting caries. However, the mechanism of SDF is to be elucidated. Aim. To characterize the effects of SDF on dentine carious induced by Streptococcus mutans and Actinomyces naeslundii. Design. Thirty-two artificially demineralized human dentine blocks were inoculated: 16 with S. mutans and 16 with A. naeslundii. Either SDF or water was applied to eight blocks in each group. Biofilm morphology, microbial kinetics and viability were evaluated by scanning electron microscopy, colony forming units, and confocal microscopy. The crosssection of the dentine carious lesions were assessed by microhardness testing, scanning electron microscopy with energy-dispersive x-ray spectroscopy and Fourier transform infrared spectroscopy. Results. Biofilm counts were reduced in SDF group than control (P<0.01). Surfaces of carious lesions were harder after SDF application than after water application (P<0.05), in S. mutans group, Ca and P weight percentage after SDF application than after water application (P<0.05). Lesions showed a significantly reduced level of matrix to phosphate after SDF treatment (P<0.05). Conclusion. Present study showed that SDF posses an anti-microbial activity against cariogenic biofilm of S. mutans or A. naeslundii formed on dentine surfaces. SDF slowed down demineralization of dentine. This dual activity could be the reason behind clinical success of SDF. © 2011 The Authors. International Journal of Paediatric Dentistry © 2011 BSPD, IAPD and Blackwell Publishing Ltd. | en_HK |
dc.language | eng | en_US |
dc.publisher | Wiley-Blackwell Publishing Ltd.. The Journal's web site is located at http://www.wiley.com/bw/journal.asp?ref=0960-7439 | en_HK |
dc.relation.ispartof | International Journal of Paediatric Dentistry | en_HK |
dc.rights | This is the peer reviewed version of the following article: International Journal Of Paediatric Dentistry, 2012, v. 22 n. 1, p. 2-10, which has been published in final form at https://doi.org/10.1111/j.1365-263X.2011.01149.x. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. | en_US |
dc.subject.mesh | Actinomyces - drug effects | - |
dc.subject.mesh | Cariostatic Agents - pharmacology | - |
dc.subject.mesh | Dental Caries - microbiology - pathology - prevention and control | - |
dc.subject.mesh | Dentin - drug effects - microbiology - pathology - ultrastructure | - |
dc.subject.mesh | Quaternary Ammonium Compounds - pharmacology | - |
dc.title | Effects of silver diamine fluoride on dentine carious lesions induced by Streptococcus mutans and Actinomyces naeslundii biofilms | en_HK |
dc.type | Article | en_HK |
dc.identifier.email | Chu, CH:chchu@hku.hk | en_HK |
dc.identifier.email | Seneviratne, CJ:jaya@hku.hk | en_HK |
dc.identifier.email | Lo, ECM:hrdplcm@hkucc.hku.hk | en_HK |
dc.identifier.authority | Chu, CH=rp00022 | en_HK |
dc.identifier.authority | Seneviratne, CJ=rp01372 | en_HK |
dc.identifier.authority | Lo, ECM=rp00015 | en_HK |
dc.description.nature | postprint | - |
dc.identifier.doi | 10.1111/j.1365-263X.2011.01149.x | en_HK |
dc.identifier.pmid | 21702854 | - |
dc.identifier.scopus | eid_2-s2.0-83555166203 | en_HK |
dc.identifier.hkuros | 191870 | en_US |
dc.identifier.hkuros | 200147 | - |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-83555166203&selection=ref&src=s&origin=recordpage | en_HK |
dc.identifier.volume | 22 | en_HK |
dc.identifier.issue | 1 | en_HK |
dc.identifier.spage | 2 | en_HK |
dc.identifier.epage | 10 | en_HK |
dc.identifier.eissn | 1365-263X | - |
dc.identifier.isi | WOS:000297954300002 | - |
dc.publisher.place | United Kingdom | en_HK |
dc.relation.project | Effects of silver diamine fluoride on enamel and dentin with caries-like lesions against biofilm challenge | - |
dc.identifier.scopusauthorid | Chu, CH=7404345729 | en_HK |
dc.identifier.scopusauthorid | Mei, L=54780178100 | en_HK |
dc.identifier.scopusauthorid | Seneviratne, CJ=6701897753 | en_HK |
dc.identifier.scopusauthorid | Lo, ECM=7101705982 | en_HK |
dc.identifier.issnl | 0960-7439 | - |