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Article: Reduction of GTP activation of adenylate cyclase system by its coupling to hormone receptor
Title | Reduction of GTP activation of adenylate cyclase system by its coupling to hormone receptor |
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Authors | |
Issue Date | 1979 |
Publisher | American Society for Biochemistry and Molecular Biology, Inc. The Journal's web site is located at http://www.jbc.org/ |
Citation | Journal Of Biological Chemistry, 1979, v. 254 n. 11, p. 4684-4688 How to Cite? |
Abstract | We have examined the characteristics of the adenylate cyclase system from control and butyrate-treated cells. Butyrate treatment results in both an increased number of catecholamine receptors and an induction of a response to the hormone, as reported previously (Tallman, J. F. Smith, C. C., and Henneberry, R. C. (1977) Proc. Natl. Acad. Sci. U. S. A. 74, 873-877); in addition, we found that the same treatment reduces the degree of activation of adenylate cyclase by GTP. We have demonstrated in two cell types that this decrease in GTP activation is inversely related to the degree of induction of the hormone response. Furthermore, in plasma membranes isolated from butyrate-treated cells, the hormone receptor is sensitive to GTP; i.e. GTP reduces the affinity of isoproterenol for the receptor. We propose that these changes reflect an interaction between the β-adrenergic receptor and the nucleotide regulatory component and that this interaction represents, at least in part, the process of coupling. Several possible mechanisms which can account for the change in GTP activation are discussed in terms of our current understanding of the regulation of the adenylate cyclase system. |
Persistent Identifier | http://hdl.handle.net/10722/167448 |
ISSN | 2020 Impact Factor: 5.157 2023 SCImago Journal Rankings: 1.766 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Lin, MC | en_US |
dc.contributor.author | Lin, C | en_US |
dc.contributor.author | Whitlock Jr, JP | en_US |
dc.date.accessioned | 2012-10-08T03:07:08Z | - |
dc.date.available | 2012-10-08T03:07:08Z | - |
dc.date.issued | 1979 | en_US |
dc.identifier.citation | Journal Of Biological Chemistry, 1979, v. 254 n. 11, p. 4684-4688 | en_US |
dc.identifier.issn | 0021-9258 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/167448 | - |
dc.description.abstract | We have examined the characteristics of the adenylate cyclase system from control and butyrate-treated cells. Butyrate treatment results in both an increased number of catecholamine receptors and an induction of a response to the hormone, as reported previously (Tallman, J. F. Smith, C. C., and Henneberry, R. C. (1977) Proc. Natl. Acad. Sci. U. S. A. 74, 873-877); in addition, we found that the same treatment reduces the degree of activation of adenylate cyclase by GTP. We have demonstrated in two cell types that this decrease in GTP activation is inversely related to the degree of induction of the hormone response. Furthermore, in plasma membranes isolated from butyrate-treated cells, the hormone receptor is sensitive to GTP; i.e. GTP reduces the affinity of isoproterenol for the receptor. We propose that these changes reflect an interaction between the β-adrenergic receptor and the nucleotide regulatory component and that this interaction represents, at least in part, the process of coupling. Several possible mechanisms which can account for the change in GTP activation are discussed in terms of our current understanding of the regulation of the adenylate cyclase system. | en_US |
dc.language | eng | en_US |
dc.publisher | American Society for Biochemistry and Molecular Biology, Inc. The Journal's web site is located at http://www.jbc.org/ | en_US |
dc.relation.ispartof | Journal of Biological Chemistry | en_US |
dc.subject.mesh | 1-Methyl-3-Isobutylxanthine - Pharmacology | en_US |
dc.subject.mesh | Adenylate Cyclase - Metabolism | en_US |
dc.subject.mesh | Butyrates - Pharmacology | en_US |
dc.subject.mesh | Cell Membrane - Drug Effects - Metabolism | en_US |
dc.subject.mesh | Fluorides - Pharmacology | en_US |
dc.subject.mesh | Guanosine Triphosphate - Pharmacology | en_US |
dc.subject.mesh | Guanylyl Imidodiphosphate - Pharmacology | en_US |
dc.subject.mesh | Hela Cells - Drug Effects - Metabolism | en_US |
dc.subject.mesh | Humans | en_US |
dc.subject.mesh | Isoproterenol - Pharmacology | en_US |
dc.subject.mesh | Kinetics | en_US |
dc.subject.mesh | Receptors, Cyclic Amp - Drug Effects - Metabolism | en_US |
dc.title | Reduction of GTP activation of adenylate cyclase system by its coupling to hormone receptor | en_US |
dc.type | Article | en_US |
dc.identifier.email | Lin, MC:mcllin@hkucc.hku.hk | en_US |
dc.identifier.authority | Lin, MC=rp00746 | en_US |
dc.description.nature | link_to_OA_fulltext | en_US |
dc.identifier.pmid | 86543 | - |
dc.identifier.scopus | eid_2-s2.0-0018759456 | en_US |
dc.identifier.volume | 254 | en_US |
dc.identifier.issue | 11 | en_US |
dc.identifier.spage | 4684 | en_US |
dc.identifier.epage | 4688 | en_US |
dc.identifier.isi | WOS:A1979GZ30300064 | - |
dc.publisher.place | United States | en_US |
dc.identifier.scopusauthorid | Lin, MC=7404816359 | en_US |
dc.identifier.scopusauthorid | Lin, C=24540146100 | en_US |
dc.identifier.scopusauthorid | Whitlock Jr, JP=7102828708 | en_US |
dc.identifier.issnl | 0021-9258 | - |